Recombinant human TYRO3 protein (Active) is a Human Fragment protein, in the 455 to 890 aa range, expressed in Baculovirus infected Sf9, with >=55% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >=55% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
R K E T R F G Q A F D S V M A R G E P A V H F R A A R S F N R E R P E R I E A T L D S L G I S D E L K E K L E D V L I P E Q Q F T L G R M L G K G E F G S V R E A Q L K Q E D G S F V K V A V K M L K A D I I A S S D I E E F L R E A A C M K E F D H P H V A K L V G V S L R S R A K G R L P I P M V I L P F M K H G D L H A F L L A S R I G E N P F N L P L Q T L I R F M V D I A C G M E Y L S S R N F I H R D L A A R N C M L A E D M T V C V A D F G L S R K I Y S G D Y Y R Q G C A S K L P V K W L A L E S L A D N L Y T V Q S D V W A F G V T M W E I M T R G Q T P Y A G I E N A E I Y N Y L I G G N R L K Q P P E C M E D V Y D L M Y Q C W S A D P K Q R P S F T C L R M E L E N I L G Q L S V L S A S Q D P L Y I N I E R A E E P T A G G S L E L P G R D Q P Y S G A G D G S G M G A V G G T P S D C R Y I L T P G G L A E Q P G Q A E H Q P E S P L N E T Q R L L L L Q Q G L L P H S S C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope. (Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.
Alternative names
BYK, DTK, RSE, SKY, TIF, TYRO3, Tyrosine-protein kinase receptor TYRO3, Tyrosine-protein kinase BYK, Tyrosine-protein kinase DTK, Tyrosine-protein kinase RSE, Tyrosine-protein kinase SKY, Tyrosine-protein kinase TIF
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Recombinant human TYRO3 protein (Active) is a Human Fragment protein, in the 455 to 890 aa range, expressed in Baculovirus infected Sf9, with >=55% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=55% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: 140 nmol/min/mg.
Assay Conditions: Assay was done in Kinase buffer containing 2 mM DTT using Poly-(Glu4:Tyr) substrate (0.2 mg/ml) and 20 μM ATP. Reaction was performed at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent.
- Accession
- Q06418-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% Glutathione, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- R K E T R F G Q A F D S V M A R G E P A V H F R A A R S F N R E R P E R I E A T L D S L G I S D E L K E K L E D V L I P E Q Q F T L G R M L G K G E F G S V R E A Q L K Q E D G S F V K V A V K M L K A D I I A S S D I E E F L R E A A C M K E F D H P H V A K L V G V S L R S R A K G R L P I P M V I L P F M K H G D L H A F L L A S R I G E N P F N L P L Q T L I R F M V D I A C G M E Y L S S R N F I H R D L A A R N C M L A E D M T V C V A D F G L S R K I Y S G D Y Y R Q G C A S K L P V K W L A L E S L A D N L Y T V Q S D V W A F G V T M W E I M T R G Q T P Y A G I E N A E I Y N Y L I G G N R L K Q P P E C M E D V Y D L M Y Q C W S A D P K Q R P S F T C L R M E L E N I L G Q L S V L S A S Q D P L Y I N I E R A E E P T A G G S L E L P G R D Q P Y S G A G D G S G M G A V G G T P S D C R Y I L T P G G L A E Q P G Q A E H Q P E S P L N E T Q R L L L L Q Q G L L P H S S C
- Accession
- Q06418
- Protein length
- Fragment
- Predicted molecular weight
- 77 kDa
- Amino acids
- 455 to 890
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
- Post-translational modifications
Autophosphorylated.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TYRO3 also known as Tyrosine-protein kinase receptor TYRO3 is a membrane protein involved in various cellular processes. The protein has a molecular mass of approximately 104 kDa. TYRO3 expresses in a range of tissues with high levels noted in the central nervous system reproductive system and immune cells. The protein is part of the TAM family of receptor tyrosine kinases which includes AXL and MERTK and it participates in binding to specific ligands for signal transmission.
- Biological function summary
TYRO3 influences multiple cellular activities such as cell survival proliferation and immune regulation. It acts within a signaling complex that includes ligands like Gas6 and Protein S. TYRO3 plays roles in phagocytosis of apoptotic cells and regulation of inflammatory responses. The protein's signaling pathways support tissue homeostasis and modulate immune responses making it an important player in maintaining cellular balance.
- Pathways
TYRO3 is engaged in the PI3K/AKT and MAPK pathways which facilitate cell cycle progression and survival. In these pathways it interacts with other proteins such as AXL MERTK and downstream effectors like AKT and ERK. These interactions ensure proper cellular functions with TYRO3-mediated pathways contributing to normal cellular homeostasis and protective responses against stress.
- Associated diseases and disorders
Disruptions in TYRO3 function associate with cancer and autoimmune diseases. Its overexpression or mutations can lead to abnormal cell growth linking TYRO3 to certain cancers. The protein also intersects with immune-related disorders where an imbalance in TYRO3 signaling contributes to diseases like systemic lupus erythematosus. Proteins like AXL and MERTK closely related to TYRO3 often share these pathological associations emphasizing the interconnected nature of the TAM receptor family in disease pathogenesis.
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2 product images
Functional Studies - Recombinant human TYRO3 protein (Active) (ab271778)
Specific activity of ab271778 was 140 nmol/min/mg in a kinase assay using Poly-(Glu4:Tyr) as substrate.
SDS-PAGE - Recombinant human TYRO3 protein (Active) (ab271778)
SDS-PAGE analysis of ab271778.
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