Recombinant Human Tyrosyl tRNA synthetase/TyrRS protein is a Human Full Length protein, in the 1 to 528 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G D A P S P E E K L H L I T R N L Q E V L G E E K L K E I L K E R E L K I Y W G T A T T G K P H V A Y F V P M S K I A D F L K A G C E V T I L F A D L H A Y L D N M K A P W E L L E L R V S Y Y E N V I K A M L E S I G V P L E K L K F I K G T D Y Q L S K E Y T L D V Y R L S S V V T Q H D S K K A G A E V V K Q V E H P L L S G L L Y P G L Q A L D E E Y L K V D A Q F G G I D Q R K I F T F A E K Y L P A L G Y S K R V H L M N P M V P G L T G S K M S S S E E E S K I D L L D R K E D V K K K L K K A F C E P G N V E N N G V L S F I K H V L F P L K S E F V I L R D E K W G G N K T Y T A Y V D L E K D F A A E V V H P G D L K N S V E V A L N K L L D P I R E K F N T P A L K K L A S A A Y P D P S K Q K P M A K G P A K N S E P E E V I P S R L D I R V G K I I T V E K H P D A D S L Y V E K I D V G E A E P R T V V S G L V Q F V P K E E L Q D R L V V V L C N L K P Q K M R G V E S Q G M L L C A S I E G I N R Q V E P L D P P A G S A P G E H V F V K G Y E K G Q P D E E L K P K K K V F E K L Q A D F K I S E E C I A Q W K Q T N F M T K L G S I S C K S L K G G N I S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Tyrosine--tRNA ligase that catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) (Probable) (PubMed:25533949). Also acts as a positive regulator of poly-ADP-ribosylation in the nucleus, independently of its tyrosine--tRNA ligase activity (PubMed:25533949). Activity is switched upon resveratrol-binding: resveratrol strongly inhibits the tyrosine--tRNA ligase activity and promotes relocalization to the nucleus, where YARS1 specifically stimulates the poly-ADP-ribosyltransferase activity of PARP1 (PubMed:25533949).
Alternative names
YARS, YARS1, Tyrosyl-tRNA synthetase, TyrRS
Recommended products
Recombinant Human Tyrosyl tRNA synthetase/TyrRS protein is a Human Full Length protein, in the 1 to 528 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P54577-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G D A P S P E E K L H L I T R N L Q E V L G E E K L K E I L K E R E L K I Y W G T A T T G K P H V A Y F V P M S K I A D F L K A G C E V T I L F A D L H A Y L D N M K A P W E L L E L R V S Y Y E N V I K A M L E S I G V P L E K L K F I K G T D Y Q L S K E Y T L D V Y R L S S V V T Q H D S K K A G A E V V K Q V E H P L L S G L L Y P G L Q A L D E E Y L K V D A Q F G G I D Q R K I F T F A E K Y L P A L G Y S K R V H L M N P M V P G L T G S K M S S S E E E S K I D L L D R K E D V K K K L K K A F C E P G N V E N N G V L S F I K H V L F P L K S E F V I L R D E K W G G N K T Y T A Y V D L E K D F A A E V V H P G D L K N S V E V A L N K L L D P I R E K F N T P A L K K L A S A A Y P D P S K Q K P M A K G P A K N S E P E E V I P S R L D I R V G K I I T V E K H P D A D S L Y V E K I D V G E A E P R T V V S G L V Q F V P K E E L Q D R L V V V L C N L K P Q K M R G V E S Q G M L L C A S I E G I N R Q V E P L D P P A G S A P G E H V F V K G Y E K G Q P D E E L K P K K K V F E K L Q A D F K I S E E C I A Q W K Q T N F M T K L G S I S C K S L K G G N I S
- Accession
- P54577
- Protein length
- Full Length
- Predicted molecular weight
- 61.3 kDa
- Amino acids
- 1 to 528
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab107141 is purified using conventional chromatography techniques.
General info
- Function
Tyrosine--tRNA ligase that catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) (Probable) (PubMed:25533949). Also acts as a positive regulator of poly-ADP-ribosylation in the nucleus, independently of its tyrosine--tRNA ligase activity (PubMed:25533949). Activity is switched upon resveratrol-binding: resveratrol strongly inhibits the tyrosine--tRNA ligase activity and promotes relocalization to the nucleus, where YARS1 specifically stimulates the poly-ADP-ribosyltransferase activity of PARP1 (PubMed:25533949).
- Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as Tyrosyl tRNA synthetase
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tyrosyl tRNA synthetase also known as TyrRS is an enzyme responsible for charging tRNA molecules with the amino acid tyrosine. This process known as aminoacylation is essential for accurate translation of the genetic code during protein synthesis. TyrRS has a molecular weight of approximately 58 kDa. It is expressed in various tissues playing a significant role in both cytoplasmic and mitochondrial protein synthesis in eukaryotes.
- Biological function summary
TyrRS plays an important role in ensuring the fidelity of protein translation within cells. It facilitates the correct pairing of tRNA^Tyr with its corresponding tyrosine amino acid contributing to the accuracy of protein production. This enzyme is part of the aminoacyl-tRNA synthetase complex a multi-enzyme complex that catalyzes the attachment of specific amino acids to their appropriate tRNA molecules an important step in the translation process.
- Pathways
TyrRS is involved in the protein synthesis pathway where it functions alongside other aminoacyl-tRNA synthetases to help maintain the genetic code's accuracy. Additionally TyrRS is implicated in the aminoacyl-tRNA biosynthesis pathway connecting its activity with enzymes responsible for similar processes. It works closely with related enzymes such as phenylalanyl-tRNA synthetase (PheRS) and tryptophanyl-tRNA synthetase (TrpRS) through these pathways ensuring coordinated protein synthesis.
- Associated diseases and disorders
TyrRS has been connected to certain neurodegenerative diseases and cancer. Mutations in the TYRRS gene have been associated with Charcot-Marie-Tooth disease a hereditary neuropathy. The enzyme's altered function in this context may lead to compromised protein synthesis. Additionally aberrant TyrRS expression has been observed in some types of cancer where it interacts with proteins like angiogenin to potentially promote tumor growth. These connections highlight the enzyme's potential role in disease progression and pathogenesis.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human Tyrosyl tRNA synthetase/TyrRS protein (ab107141)
15% SDS-PAGE showing ab107141 (3 μg) at approximately 61.3 kDa.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com