JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB117010

Recombinant Human U1-C protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human U1-C protein is a Human Full Length protein, in the 1 to 159 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

U1 small nuclear ribonucleoprotein C, U1 snRNP C, U1-C, U1C, SNRPC

1 Images
SDS-PAGE - Recombinant Human U1-C protein (AB117010)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human U1-C protein (AB117010)

12.5% SDS-PAGE showing ab117010 at approximately 43.56 kDa.
Stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

P09234

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Recombinant protein.</p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MPKFYCDYCDTYLTHDSPSVRKTHCSGRKHKENVKDYYQKWMEEQAQSLIDKTTAAFQQGKIPPTPFSAPPPAGAMIPPPPSLPGPPRPGMMPAPHMGGPPMMPMMGPPPPGMMPVGPAPGMRPPMGGHMPMMPGPPMMRPPARPMMVPTRPGMTRPDR","proteinLength":"Full Length","predictedMolecularWeight":"43.56 kDa","actualMolecularWeight":null,"aminoAcidEnd":159,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P09234","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

U1-C also known as U1 small nuclear ribonucleoprotein C is a component of the U1 snRNP complex. It has a molecular mass of approximately 17 kDa and is expressed in the nucleus of eukaryotic cells. This protein plays an essential role in the spliceosome machinery which is responsible for pre-mRNA splicing. U1-C is part of a highly conserved group of proteins that engage in RNA processing and modification.
Biological function summary

U1-C interacts with U1 snRNA and other proteins to form the U1 snRNP an essential component of the spliceosome complex. The spliceosome removes introns from pre-mRNA facilitating the production of mature mRNA. U1-C stabilizes the interaction between the 5' splice site of pre-mRNA and the snRNP playing a significant part in the accuracy of splicing. Its precise function in RNA stability and processing makes it an important aspect of gene expression regulation.

Pathways

U1-C participates in the splicing pathway ensuring the correct removal of introns during RNA maturation. This protein associates closely with U1 snRNA and other spliceosomal proteins such as U1-70K. It plays a role in the regulation of alternative splicing pathways which can affect gene expression patterns. The U1-C protein indirectly influences processes like cell cycle progression and differentiation through its involvement in RNA splicing.

Malfunction or mutation of U1-C can lead to splicing abnormalities which are linked to diseases such as systemic lupus erythematosus and certain cancers. In lupus autoantibodies target the U1 snRNP complex including U1-C leading to immune system dysfunction. This association highlights the importance of U1-C in maintaining cellular and immune system homeostasis. Additionally aberrant splicing facilitated by altered U1-C function may impact oncogene or tumor suppressor pathways contributing to cancer development.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRPC/U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing. The interaction with the 5' splice-site seems to precede base-pairing between the pre-mRNA and the U1 snRNA. Stimulates commitment or early (E) complex formation by stabilizing the base pairing of the 5' end of the U1 snRNA and the 5' splice-site region.

Sequence similarities

Belongs to the U1 small nuclear ribonucleoprotein C family.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRPC/U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing. The interaction with the 5' splice-site seems to precede base-pairing between the pre-mRNA and the U1 snRNA. Stimulates commitment or early (E) complex formation by stabilizing the base pairing of the 5' end of the U1 snRNA and the 5' splice-site region.
See full target information SNRPC
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB192028

Anti-U1-C antibody [EPR16034]

RabMAb|Recombinant|Trial Size

Western blot - Anti-U1-C antibody [EPR16034] (AB192028)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com