JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB132467

Recombinant Human U1A protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human U1A protein is a Human Full Length protein, in the 1 to 282 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

U1 small nuclear ribonucleoprotein A, U1 snRNP A, U1-A, U1A, SNRPA

1 Images
SDS-PAGE - Recombinant Human U1A protein (AB132467)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human U1A protein (AB132467)

SDS-PAGE analysis of ab132467 on a 12.5% gel stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

P09012

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(Recombinant protein)</p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MAVPETRPNHTIYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGTFVERDRKREKRKPKSQETPATKKAVQGGGATPVVGAVQGPVPGMPPMTQAPRIMHHMPGQPPYMPPPGMIPPPGLAPGQIPPGAMPPQQLMPGQMPPAQPLSENPPNHILFLTNLPEETNELMLSMLFNQFPGFKEVRLVPGRHDIAFVEFDNEVQAGAARDALQGFKITQNNAMKISFAKK","proteinLength":"Full Length","predictedMolecularWeight":"56.76 kDa","actualMolecularWeight":null,"aminoAcidEnd":282,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P09012","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

U1A also known as snRNP U1A is a protein involved in the splicing of pre-messenger RNA. U1A is approximately 31 kDa in mass and functions as an important component of the U1 small nuclear ribonucleoprotein (snRNP) complex. Expressed in the cell nucleus U1A binds to the stem-loop structures of U1 RNA stabilizing the RNA-protein complex. This binding activity is critical as it allows U1 snRNP to recognize and bind splice sites on precursor mRNA facilitating the splicing process.
Biological function summary

U1A participates in the formation and function of the spliceosome a large RNA-protein complex essential for pre-mRNA processing. U1A as part of the U1 snRNP helps in recognizing and positioning the 5' splice site of introns in pre-mRNA. Through this role U1A ensures proper removal of non-coding sequences allowing for the maturation of mRNA. The spliceosome which includes other components like U2 U4 U5 and U6 snRNPs coordinates finely to execute the precise cutting and ligation of RNA sequences during splicing.

Pathways

U1A plays a central role in the mRNA splicing pathway which is a critical step in gene expression regulation. The splicing pathway interfaces with other biological pathways such as those involved in mRNA translation and degradation. U1A interacts with several proteins within these pathways notably SR proteins and hnRNPs which influence splice site selection and enhance the efficiency of splicing. These interactions highlight the interconnectedness of RNA processing events with overall gene expression controls.

U1A has been implicated in autoimmune diseases such as systemic lupus erythematosus (SLE). In SLE the body generates autoantibodies against several snRNP components including U1A which disrupts normal splicesome function. This leads to defective RNA processing and contributes to disease pathology. Additionally mutations or dysregulation of splicing proteins like SF3B1 and SRSF2 also associated with U1A through splicing abnormalities have been linked to various cancers where they lead to aberrant splicing patterns that drive tumorigenesis.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs.

Sequence similarities

Belongs to the RRM U1 A/B'' family.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs.
See full target information SNRPA
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB116200

Recombinant Human SNRPA1 protein

SDS-PAGE - Recombinant Human SNRPA1 protein (AB116200)

  • 0
  • 0
Primary Antibodies

AB202140

Anti-U1A antibody

Immunocytochemistry/ Immunofluorescence - Anti-U1A antibody (AB202140)

  • 0
  • 0
Proteins & Peptides

AB276570

Recombinant Human CYTL1 protein (Fc Chimera)

SDS-PAGE - Recombinant Human CYTL1 protein (Fc Chimera) (AB276570)

  • 0
  • 0
Proteins & Peptides

AB181895

Recombinant Human SDF2 protein (denatured)

SDS-PAGE - Recombinant Human SDF2 protein (denatured) (AB181895)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com