Recombinant Human U2AF35/U2AF1 protein (denatured) is a Human Full Length protein, in the 1 to 240 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A E Y L A S I F G T E K D K V N C S F Y F K I G A C R H G D R C S R L H N K P T F S Q T I L I Q N I Y R N P Q N S A Q T A D G S H C A V S D V E M Q E H Y D E F F E E V F T E M E E K Y G E V E E M N V C D N L G D H L V G N V Y V K F R R E E D A E K A V I D L N N R W F N G Q P I H A E L S P V T D F R E A C C R Q Y E M G E C T R G G F C N F M H L K P I S R E L R R E L Y G R R R K K H R S R S R S R E R R S R S R D R G R G G G G G G G G G G G G R E R D R R R S R D R E R S G R F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
Alternative names
U2AF35, U2AFBP, FP793, U2AF1, Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliary factor small subunit
Recommended products
Recombinant Human U2AF35/U2AF1 protein (denatured) is a Human Full Length protein, in the 1 to 240 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q01081-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A E Y L A S I F G T E K D K V N C S F Y F K I G A C R H G D R C S R L H N K P T F S Q T I L I Q N I Y R N P Q N S A Q T A D G S H C A V S D V E M Q E H Y D E F F E E V F T E M E E K Y G E V E E M N V C D N L G D H L V G N V Y V K F R R E E D A E K A V I D L N N R W F N G Q P I H A E L S P V T D F R E A C C R Q Y E M G E C T R G G F C N F M H L K P I S R E L R R E L Y G R R R K K H R S R S R S R E R R S R S R D R G R G G G G G G G G G G G G R E R D R R R S R D R E R S G R F
- Accession
- Q01081
- Protein length
- Full Length
- Predicted molecular weight
- 30.3 kDa
- Amino acids
- 1 to 240
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
- Sequence similarities
Belongs to the splicing factor SR family.
- Subcellular localisation
- Nucleus, Nucleus speckle
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as U2AF35
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
U2AF35 also known as U2AF1 is a protein weighing approximately 35 kDa. It interacts importantly in the pre-mRNA splicing process where it binds to the 3' splice site of introns. The protein is a subunit of the U2 auxiliary factor and its expression occurs across various tissues with particular concentrations in the developmental stages of tissues and in dynamic cellular environments. U2AF35 functions by recognizing the AG dinucleotide at the 3' end of introns a task critical for the accurate splicing of precursor mRNA.
- Biological function summary
This protein plays an important role in the removal of introns from pre-mRNA impacting the processing of mRNA which ultimately affects gene expression. U2AF35 is a component of the spliceosome complex particularly influencing the recruitment of U2 small nuclear ribonucleoprotein (snRNP) to the branch point. The activity of U2AF35 ensures proper mRNA maturation which is essential in controlling the flow of genetic information from DNA to protein synthesis. It also contributes to the alternative splicing allowing cells to produce diverse protein isoforms from a single gene.
- Pathways
U2AF35 is critically involved in the mRNA splicing pathway and the regulation of alternative splicing events. U2AF35 partners with another subunit U2AF65 in the spliceosome to facilitate these processes. It actively connects with several other proteins within the spliceosome machinery including SF3b and SF3a complexes. The interaction between U2AF35 and these elements ensures the correct assembly of the spliceosome directly influencing the pathway governing pre-mRNA processing.
- Associated diseases and disorders
Mutations in the U2AF35 gene can result in specific types of cancer such as myelodysplastic syndromes (MDS) where abnormal splicing patterns occur. Such mutations often affect the interaction of U2AF35 with other splicing factors like SF3B1. Additionally alterations in U2AF35 have associations with lung adenocarcinoma where changes in the mRNA splicing landscape empower cancer progression and malignancy. Understanding its role in splicing provides insight into how disruptions can lead to disease development.
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1 product image
SDS-PAGE - Recombinant Human U2AF35/U2AF1 protein (denatured) (ab156324)
15% SDS-PAGE analysis of ab156324 (3μg).
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