Recombinant Human UBA2 protein (His tag) is a Human Full Length protein, in the 1 to 640 aa range, expressed in Baculovirus infected insect, with >85% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A D L M A L S R G L P R E L A E A V A G G R V L V V G A G G I G C E L L K N L V L T G F S H I D L I D L D T I D V S N L N R Q F L F Q K K H V G R S K A Q V A K E S V L Q F Y P K A N I V A Y H D S I M N P D Y N V E F F R Q F I L V M N A L D N R A A R N H V N R M C L A A D V P L I E S G T A G Y L G Q V T T I K K G V T E C Y E C H P K P T Q R T F P G C T I R N T P S E P I H C I V W A K Y L F N Q L F G E E D A D Q E V S P D R A D P E A A W E P T E A E A R A R A S N E D G D I K R I S T K E W A K S T G Y D P V K L F T K L F K D D I R Y L L T M D K L W R K R K P P V P L D W A E V Q S Q G E E T N A S D Q Q N E P Q L G L K D Q Q V L D V K S Y A R L F S K S I E T L R V H L A E K G D G A E L I W D K D D P S A M D F V T S A A N L R M H I F S M N M K S R F D I K S M A G N I I P A I A T T N A V I A G L I V L E G L K I L S G K I D Q C R T I F L N K Q P N P R K K L L V P C A L D P P N P N C Y V C A S K P E V T V R L N V H K V T V L T L Q D K I V K E K F A M V A P D V Q I E D G K G T I L I S S E E G E T E A N N H K K L S E F G I R N G S R L Q A D D F L Q D Y T L L I N I L H S E D L G K D V E F E V V G D A P E K V G P K Q A E D A A K S I T N G S D D G A Q P S T S T A Q E Q D D V L I V D S D E E D S S N N A D V S E E E R S R K R K L D E K E N L S A K R S R I E Q K E E L D D V I A L D H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes MW: 70-100 kDa (SDS-PAGE under reducing conditions) |
Target data
Function
The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Alternative names
SAE2, UBLE1B, HRIHFB2115, UBA2, SUMO-activating enzyme subunit 2, Anthracycline-associated resistance ARX, Ubiquitin-like 1-activating enzyme E1B, Ubiquitin-like modifier-activating enzyme 2
Recommended products
Recombinant Human UBA2 protein (His tag) is a Human Full Length protein, in the 1 to 640 aa range, expressed in Baculovirus infected insect, with >85% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9UBT2-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A D L M A L S R G L P R E L A E A V A G G R V L V V G A G G I G C E L L K N L V L T G F S H I D L I D L D T I D V S N L N R Q F L F Q K K H V G R S K A Q V A K E S V L Q F Y P K A N I V A Y H D S I M N P D Y N V E F F R Q F I L V M N A L D N R A A R N H V N R M C L A A D V P L I E S G T A G Y L G Q V T T I K K G V T E C Y E C H P K P T Q R T F P G C T I R N T P S E P I H C I V W A K Y L F N Q L F G E E D A D Q E V S P D R A D P E A A W E P T E A E A R A R A S N E D G D I K R I S T K E W A K S T G Y D P V K L F T K L F K D D I R Y L L T M D K L W R K R K P P V P L D W A E V Q S Q G E E T N A S D Q Q N E P Q L G L K D Q Q V L D V K S Y A R L F S K S I E T L R V H L A E K G D G A E L I W D K D D P S A M D F V T S A A N L R M H I F S M N M K S R F D I K S M A G N I I P A I A T T N A V I A G L I V L E G L K I L S G K I D Q C R T I F L N K Q P N P R K K L L V P C A L D P P N P N C Y V C A S K P E V T V R L N V H K V T V L T L Q D K I V K E K F A M V A P D V Q I E D G K G T I L I S S E E G E T E A N N H K K L S E F G I R N G S R L Q A D D F L Q D Y T L L I N I L H S E D L G K D V E F E V V G D A P E K V G P K Q A E D A A K S I T N G S D D G A Q P S T S T A Q E Q D D V L I V D S D E E D S S N N A D V S E E E R S R K R K L D E K E N L S A K R S R I E Q K E E L D D V I A L D H H H H H H
- Accession
- Q9UBT2
- Protein length
- Full Length
- Predicted molecular weight
- 72.3 kDa
- Amino acids
- 1 to 640
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
- Sequence similarities
Belongs to the ubiquitin-activating E1 family.
- Post-translational modifications
Sumoylated with SUMO1 and SUMO2/3 and by UBC9. Sumoylation at Lys-236 inhibits enzymatic activity. Sumoylation at the C-terminal lysine cluster plays an essential role in nuclear trafficking.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UBA2 also known as SUMO-activating enzyme subunit 2 is a protein involved in the initial step of the SUMOylation process. It has a molecular mass of approximately 72 kDa. UBA2 functions as part of the enzyme complex that activates small ubiquitin-like modifier (SUMO) proteins. Its expression shows wide variability among tissues with higher levels found in the heart and skeletal muscle compared to other tissues.
- Biological function summary
This enzyme holds major importance in the regulation of protein activity and stability. UBA2 forms a heterodimeric complex with SAE1 (SUMO-activating enzyme subunit 1) where it plays a catalytic role in the adenylation of SUMO facilitating its attachment to target proteins. This SUMOylation regulates several cellular processes including nuclear transport transcriptional regulation and response to stress adding a versatile layer to protein function.
- Pathways
UBA2 is directly involved in the SUMOylation pathway an important post-translational modification pathway shouldering various cellular functions. This protein partners with SAE1 forming the SUMO-activating enzyme E1 complex initiating the SUMOylation cascade. The pathway shares interactions with ubiquitin-like modification pathways demonstrating functional crosstalk with proteins such as UBC9 which loads SUMO onto substrates and PIAS molecules acting as E3 ligases.
- Associated diseases and disorders
The activity of UBA2 affects the regulation of processes linked to cancer and neurodegenerative diseases. Altered SUMOylation where UBA2 plays a catalytic role can lead to dysregulation in protein function and signaling pathways contributing to tumor proliferative behaviors in cancers. In connection with Huntington’s disease modified SUMOylation may alter the aggregation of huntingtin protein influencing disease progression. Understanding the role of UBA2 and interactions with proteins such as RANGAP1 offers insights into potential therapeutic avenues.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human UBA2 protein (His tag) (ab219280)
15% SDS-PAGE - Recombinant Human UBA2 protein (His tag) (ab219280) at 3 μg.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com