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AB123194

Recombinant Human UBA3 protein

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Recombinant Human UBA3 protein is a Human Full Length protein, in the 1 to 463 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

UBE1C, UBA3, NEDD8-activating enzyme E1 catalytic subunit, NEDD8-activating enzyme E1C, Ubiquitin-activating enzyme E1C, Ubiquitin-like modifier-activating enzyme 3, Ubiquitin-activating enzyme 3

1 Images
SDS-PAGE - Recombinant Human UBA3 protein (AB123194)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human UBA3 protein (AB123194)

15% SDS-PAGE showing ab123194 (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q8TBC4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as UBE1C
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMADGEEPERKRRRIEELLAEKMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS","proteinLength":"Full Length","predictedMolecularWeight":"54.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":463,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8TBC4","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target UBA3 also known as ubiquitin-like modifier-activating enzyme 3 functions mechanically as an essential enzyme in the NEDD8-conjugation pathway. It weighs approximately 50 kDa. UBA3 expresses itself in various tissues but shows widespread expression across different cell types. It operates as a catalytic subunit of the E1 enzyme complex that initiates the activation and transfer of the NEDD8 protein. This process is important for the subsequent conjugation to target proteins.
Biological function summary

The process involving UBA3 relates to protein modification primarily through neddylation. UBA3 couples with the protein NAE1 to form the NEDD8 activating enzyme complex. This enzyme complex activates NEDD8 a ubiquitin-like protein that modifies cullin-based E3 ligases influencing their function. By modifying these E3 ligases UBA3 controls various cellular processes including cell cycle progression and signal transduction ensuring proper cell function and adaptation to environmental cues.

Pathways

UBA3 is integral to the NEDD8 conjugation pathway influencing cell cycle and transcriptional regulation. It interacts with proteins such as cullin 1 and cullin 3 within this pathway. By activating NEDD8 UBA3 facilitates cullin-RING ubiquitin ligase complexes which play significant roles in the ubiquitin-proteasome pathway. This interaction controls protein degradation a vital process for maintaining protein homeostasis and preventing cellular dysfunction.

UBA3 involvement in neddylation links it to cancer and neurodegenerative diseases. Aberrant UBA3 activity relates to cancers as dysregulation of the NEDD8 pathway can lead to uncontrolled cell proliferation. In neurodegenerative disorders such as Alzheimer's disease altered neddylation can disrupt normal protein homeostasis contributing to neuron damage. UBA3's interaction with protein partners like cullins connects it to various pathological states highlighting its importance in disease development and progression.
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Specifications

Form

Liquid

Additional notes

ab123194 is purified using conventional chromatography techniques.

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General info

Function

Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.

Sequence similarities

Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.

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Product protocols

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Target data

Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.
See full target information UBA3
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