Recombinant Human UBA5 protein is a Human Full Length protein, in the 1 to 404 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M A E S V E R L Q Q R V Q E L E R E L A Q E R S L Q V P R S G D G G G G R V R I E K M S S E V V D S N P Y S R L M A L K R M G I V S D Y E K I R T F A V A I V G V G G V G S V T A E M L T R C G I G K L L L F D Y D K V E L A N M N R L F F Q P H Q A G L S K V Q A A E H T L R N I N P D V L F E V H N Y N I T T V E N F Q H F M D R I S N G G L E E G K P V D L V L S C V D N F E A R M T I N T A C N E L G Q T W M E S G V S E N A V S G H I Q L I I P G E S A C F A C A P P L V V A A N I D E K T L K R E G V C A A S L P T T M G V V A G I L V Q N V L K F L L N F G T V S F Y L G Y N A M Q D F F P T M S M K P N P Q C D D R N C R K Q Q E E Y K K K V A A L P K Q E V I Q E E E E I I H E D N E W G I E L V S E V S E E E L K N F S G P V P D L P E G I T V A Y T I P K K Q E D S V T E L T V E D S G E S L E D L M A K M K N M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
E1-like enzyme which specifically catalyzes the first step in ufmylation (PubMed:15071506, PubMed:18442052, PubMed:20368332, PubMed:25219498, PubMed:26929408, PubMed:27545674, PubMed:27545681, PubMed:27653677, PubMed:30412706, PubMed:30626644, PubMed:34588452). Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP (PubMed:20368332, PubMed:26929408, PubMed:27653677, PubMed:30412706). Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer (PubMed:27653677). Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding (PubMed:29295865). Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism (PubMed:27653677, PubMed:34588452). Ufmylation plays a key role in various processes, such as ribosome recycling, response to DNA damage, interferon response or reticulophagy (also called ER-phagy) (PubMed:30412706, PubMed:32160526, PubMed:35394863). Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes (By similarity).
Alternative names
UBE1DC1, UBA5, Ubiquitin-like modifier-activating enzyme 5, Ubiquitin-activating enzyme 5, ThiFP1, UFM1-activating enzyme, Ubiquitin-activating enzyme E1 domain-containing protein 1
Recommended products
Recombinant Human UBA5 protein is a Human Full Length protein, in the 1 to 404 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9GZZ9-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.29% Sodium chloride, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M A E S V E R L Q Q R V Q E L E R E L A Q E R S L Q V P R S G D G G G G R V R I E K M S S E V V D S N P Y S R L M A L K R M G I V S D Y E K I R T F A V A I V G V G G V G S V T A E M L T R C G I G K L L L F D Y D K V E L A N M N R L F F Q P H Q A G L S K V Q A A E H T L R N I N P D V L F E V H N Y N I T T V E N F Q H F M D R I S N G G L E E G K P V D L V L S C V D N F E A R M T I N T A C N E L G Q T W M E S G V S E N A V S G H I Q L I I P G E S A C F A C A P P L V V A A N I D E K T L K R E G V C A A S L P T T M G V V A G I L V Q N V L K F L L N F G T V S F Y L G Y N A M Q D F F P T M S M K P N P Q C D D R N C R K Q Q E E Y K K K V A A L P K Q E V I Q E E E E I I H E D N E W G I E L V S E V S E E E L K N F S G P V P D L P E G I T V A Y T I P K K Q E D S V T E L T V E D S G E S L E D L M A K M K N M
- Accession
- Q9GZZ9
- Protein length
- Full Length
- Predicted molecular weight
- 47.4 kDa
- Amino acids
- 1 to 404
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab124609 is purified by using conventional chromatography techniques.
General info
- Function
E1-like enzyme which specifically catalyzes the first step in ufmylation (PubMed:15071506, PubMed:18442052, PubMed:20368332, PubMed:25219498, PubMed:26929408, PubMed:27545674, PubMed:27545681, PubMed:27653677, PubMed:30412706, PubMed:30626644, PubMed:34588452). Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP (PubMed:20368332, PubMed:26929408, PubMed:27653677, PubMed:30412706). Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer (PubMed:27653677). Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding (PubMed:29295865). Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism (PubMed:27653677, PubMed:34588452). Ufmylation plays a key role in various processes, such as ribosome recycling, response to DNA damage, interferon response or reticulophagy (also called ER-phagy) (PubMed:30412706, PubMed:32160526, PubMed:35394863). Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes (By similarity).
- Sequence similarities
Belongs to the ubiquitin-activating E1 family. UBA5 subfamily.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UBA5 also known as Ubiquitin-like modifier-activating enzyme 5 is an E1 enzyme that plays a mechanical role in activating ubiquitin-fold modifier 1 (UFM1) for protein modification. It has a molecular mass of about 44 kDa and performs its function in the nucleus and cytoplasm within human cells. The expression of UBA5 is not limited to specific tissues; instead it shows widespread expression across many tissues supporting an important role in various cellular processes.
- Biological function summary
UBA5 is implicated in the UFM1 conjugation system. This process modifies specific proteins and affects protein stability interaction and degradation. UBA5 operates in complex with UFC1 an E2 enzyme enabling the transfer of UFM1 to target proteins. Its activity is essential for maintaining cellular homeostasis particularly in the endoplasmic reticulum stress response through protein modification and regulation tasks.
- Pathways
The UBA5-UFM1 axis integrates into the cellular stress response pathway and the endoplasmic reticulum-associated degradation (ERAD) pathway. Through these pathways UBA5 collaborates with proteins like UFL1 further connecting these pathways by tagging proteins with UFM1 to mark them for degradation or functional modification. The coordination between UBA5 and UFL1 supports efficient protein quality control and maintains cellular equilibrium under stress conditions.
- Associated diseases and disorders
The dysfunction or altered expression of UBA5 associates with neurodevelopmental disorders and some cancers. Changes in UBA5 activity can lead to imbalances in protein homeostasis contributing to pathogenic mechanisms in neurodevelopmental disorders where other proteins like EIF4A1 also play a role. In cancer alterations in UBA5 expression may influence tumor progression as seen in cases where UBA5 levels affect pathways that involve p53 an important tumor suppressor protein.
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SDS-PAGE - Recombinant Human UBA5 protein (ab124609)
15% SDS-PAGE showing ab124609 (3 µg).
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