Recombinant human Uba6 protein (Active) is a Human Full Length protein, in the 1 to 1052 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >75% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M E G S E P V A A H Q G E E A S C S S W G T G S T N K N L P I M S T A S V E I D D A L Y S R Q R Y V L G D T A M Q K M A K S H V F L S G M G G L G L E I A K N L V L A G I K A V T I H D T E K C Q A W D L G T N F F L S E D D V V N K R N R A E A V L K H I A E L N P Y V H V T S S S V P F N E T T D L S F L D K Y Q C V V L T E M K L P L Q K K I N D F C R S Q C P P I K F I S A D V H G I W S R L F C D F G D E F E V L D T T G E E P K E I F I S N I T Q A N P G I V T C L E N H P H K L E T G Q F L T F R E I N G M T G L N G S I Q Q I T V I S P F S F S I G D T T E L E P Y L H G G I A V Q V K T P K T V F F E S L E R Q L K H P K C L I V D F S N P E A P L E I H T A M L A L D Q F Q E K Y S R K P N V G C Q Q D S E E L L K L A T S I S E T L E E K P D V N A D I V H W L S W T A Q G F L S P L A A A V G G V A S Q E V L K A V T G K F S P L C Q W L Y L E A A D I V E S L G K P E C E E F L P R G D R Y D A L R A C I G D T L C Q K L Q N L N I F L V G C G A I G C E M L K N F A L L G V G T S K E K G M I T V T D P D L I E K S N L N R Q F L F R P H H I Q K P K S Y T A A D A T L K I N S Q I K I D A H L N K V C P T T E T I Y N D E F Y T K Q D V I I T A L D N V E A R R Y V D S R C L A N L R P L L D S G T M G T K G H T E V I V P H L T E S Y N S H R D P P E E E I P F C T L K S F P A A I E H T I Q W A R D K F E S S F S H K P S L F N K F W Q T Y S S A E E V L Q K I Q S G H S L E G C F Q V I K L L S R R P R N W S Q C V E L A R L K F E K Y F N H K A L Q L L H C F P L D I R L K D G S L F W Q S P K R P P S P I K F D L N E P L H L S F L Q N A A K L Y A T V Y C I P F A E E D L S A D A L L N I L S E V K I Q E F K P S N K V V Q T D E T A R K P D H V P I S S E D E R N A I F Q L E K A I L S N E A T K S D L Q M A V L S F E K D D D H N G H I D F I T A A S N L R A K M Y S I E P A D R F K T K R I A G K I I P A I A T T T A T V S G L V A L E M I K V T G G Y P F E A Y K N C F L N L A I P I V V F T E T T E V R K T K I R N G I S F T I W D R W T V H G K E D F T L L D F I N A V K E K Y G I E P T M V V Q G V K M L Y V P V M P G H A K R L K L T M H K L V K P T T E K K Y V D L T V S F A P D I D G D E D L P G P P V R Y Y F S H D T D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.
Alternative names
MOP4, UBE1L2, UBA6, Ubiquitin-like modifier-activating enzyme 6, Ubiquitin-activating enzyme 6, Monocyte protein 4, Ubiquitin-activating enzyme E1-like protein 2, MOP-4, E1-L2
Recommended products
Recombinant human Uba6 protein (Active) is a Human Full Length protein, in the 1 to 1052 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >75% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab269092 was 19 nmol/min/mg in a ubiquitinating assay using wild-type ubiquitin protein as substrate.
- Accession
- A0AVT1-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M E G S E P V A A H Q G E E A S C S S W G T G S T N K N L P I M S T A S V E I D D A L Y S R Q R Y V L G D T A M Q K M A K S H V F L S G M G G L G L E I A K N L V L A G I K A V T I H D T E K C Q A W D L G T N F F L S E D D V V N K R N R A E A V L K H I A E L N P Y V H V T S S S V P F N E T T D L S F L D K Y Q C V V L T E M K L P L Q K K I N D F C R S Q C P P I K F I S A D V H G I W S R L F C D F G D E F E V L D T T G E E P K E I F I S N I T Q A N P G I V T C L E N H P H K L E T G Q F L T F R E I N G M T G L N G S I Q Q I T V I S P F S F S I G D T T E L E P Y L H G G I A V Q V K T P K T V F F E S L E R Q L K H P K C L I V D F S N P E A P L E I H T A M L A L D Q F Q E K Y S R K P N V G C Q Q D S E E L L K L A T S I S E T L E E K P D V N A D I V H W L S W T A Q G F L S P L A A A V G G V A S Q E V L K A V T G K F S P L C Q W L Y L E A A D I V E S L G K P E C E E F L P R G D R Y D A L R A C I G D T L C Q K L Q N L N I F L V G C G A I G C E M L K N F A L L G V G T S K E K G M I T V T D P D L I E K S N L N R Q F L F R P H H I Q K P K S Y T A A D A T L K I N S Q I K I D A H L N K V C P T T E T I Y N D E F Y T K Q D V I I T A L D N V E A R R Y V D S R C L A N L R P L L D S G T M G T K G H T E V I V P H L T E S Y N S H R D P P E E E I P F C T L K S F P A A I E H T I Q W A R D K F E S S F S H K P S L F N K F W Q T Y S S A E E V L Q K I Q S G H S L E G C F Q V I K L L S R R P R N W S Q C V E L A R L K F E K Y F N H K A L Q L L H C F P L D I R L K D G S L F W Q S P K R P P S P I K F D L N E P L H L S F L Q N A A K L Y A T V Y C I P F A E E D L S A D A L L N I L S E V K I Q E F K P S N K V V Q T D E T A R K P D H V P I S S E D E R N A I F Q L E K A I L S N E A T K S D L Q M A V L S F E K D D D H N G H I D F I T A A S N L R A K M Y S I E P A D R F K T K R I A G K I I P A I A T T T A T V S G L V A L E M I K V T G G Y P F E A Y K N C F L N L A I P I V V F T E T T E V R K T K I R N G I S F T I W D R W T V H G K E D F T L L D F I N A V K E K Y G I E P T M V V Q G V K M L Y V P V M P G H A K R L K L T M H K L V K P T T E K K Y V D L T V S F A P D I D G D E D L P G P P V R Y Y F S H D T D
- Accession
- A0AVT1
- Protein length
- Full Length
- Amino acids
- 1 to 1052
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.
- Sequence similarities
Belongs to the ubiquitin-activating E1 family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Uba6 also known as ubiquitin-like modifier activating enzyme 6 is an E1-like ubiquitin-activating enzyme. It has an approximate mass of around 120 kDa. This enzyme facilitates the activation of ubiquitin and similar proteins by catalyzing the formation of a ubiquitin-adenylate intermediate. Uba6 is expressed in a wide range of human tissues with higher levels observed in the brain heart and skeletal muscle. Its expression suggests a need for its activity in diverse biological processes across different tissue types.
- Biological function summary
Uba6 modifies proteins by attaching ubiquitin moieties which tags them for various fates including degradation or location changes. Uba6 functions as a part of the ubiquitin-proteasome system (UPS) a critical protein degradation and quality control pathway. In this system Uba6 collaborates with E2 conjugating enzymes such as Ube2z which transfer ubiquitin from Uba6 to substrate proteins. This involvement emphasizes its role in protein homeostasis and cellular stress responses by controlling protein stability.
- Pathways
Uba6 takes part in the maintenance of protein homeostasis and also influences the NEDD8 modification pathway. The UPS pathway integrates Uba6 with processes involving protein turnover and stress responses maintaining cellular oxidative stress levels. In the NEDD8 pathway Uba6 interacts with proteins like UBE2D which influence protein degradation scenarios that differ from those managed by the classic ubiquitin system. These pathways highlight the diverse regulatory roles Uba6 holds in managing intracellular protein dynamics.
- Associated diseases and disorders
Uba6 plays a role in neurodegenerative diseases and some cancers. In Alzheimer's disease Uba6 affects the accumulation of misfolded proteins connecting its action to the degradation pathways that include related ubiquitination enzymes. It also shows relevance to tumor progression in cancers where aberrations in ubiquitin-mediated pathways play a part in unregulated cell growth. The functions of Uba6 in these conditions link it with disorders of protein homeostasis where proteins like UBE2Z are involved.
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2 product images
Functional Studies - Recombinant human Uba6 protein (Active) (ab269092)
The specific activity of ab269092 was 19 nmol/min/mg in a ubiquitinating assay using wild-type ubiquitin protein as substrate.
SDS-PAGE - Recombinant human Uba6 protein (Active) (ab269092)
SDS-PAGE analysis of ab269092.
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