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AB167962

Recombinant Human UBC3B protein

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Recombinant Human UBC3B protein is a Human Full Length protein, in the 2 to 238 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB.

View Alternative Names

CDC34B, UBC3B, UBE2R2, Ubiquitin-conjugating enzyme E2 R2, E2 ubiquitin-conjugating enzyme R2, Ubiquitin carrier protein R2, Ubiquitin-conjugating enzyme E2-CDC34B, Ubiquitin-protein ligase R2

1 Images
SDS-PAGE - Recombinant Human UBC3B protein (AB167962)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human UBC3B protein (AB167962)

SDS Page analysis of ab167962

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

Q712K3

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"AQQQMTSSQKALMLELKSLQEEPVEGFRITLVDESDLYNWEVAIFGPPNTLYEGGYFKAHIKFPIDYPYSPPTFRFLTKMWHPNIYENGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFRKWRDSKGKDKEYAEIIRKQVSATKAEAEKDGVKVPTTLAEYCIKTKVPSNDNSSDLLYDDLYDDDIDDEDEEEEDADCYDDDDSGNEES","proteinLength":"Full Length","predictedMolecularWeight":"32 kDa","actualMolecularWeight":null,"aminoAcidEnd":238,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q712K3","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBC3B also known as UBE2E2 is a ubiquitin-conjugating enzyme with a molecular mass of approximately 25 kDa. This enzyme plays a pivotal role mechanically in the ubiquitination process where it facilitates the transfer of ubiquitin from a ubiquitin-activating enzyme to substrate proteins. UBC3B is expressed in various tissues including the brain and the reproductive system indicating its broad functional importance in different biological contexts.
Biological function summary

The enzyme takes part in protein degradation processes and regulates the stability of numerous proteins by tagging them for degradation by the proteasome. UBC3B functions as part of multi-protein ubiquitin pathways and interacts with E3 ubiquitin ligases to identify specific proteins for tagging. Its activity helps maintain cellular protein homeostasis and impacts processes like cell cycle regulation and DNA repair.

Pathways

UBC3B is involved in ubiquitin-dependent proteolysis and is critical in the cellular stress response pathways. It collaborates closely with proteins like the E3 ligase HECTD1 within these pathways. This relationship helps regulate protein turnover and the cell's response to stress ensuring damaged or misfolded proteins are efficiently degraded to prevent cellular dysfunction.

Mutations or dysregulation of UBC3B are associated with cancer and neurodegenerative diseases. In cancer the altered ubiquitin pathways can lead to uncontrolled cell proliferation due to disrupted protein degradation. UBC3B interacts with proteins like p53 a tumor suppressor linking it to oncogenic processes. In neurodegenerative diseases the accumulation of ubiquitinated proteins due to erroneous UBC3B activity can cause neuronal cell damage.
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Specifications

Form

Liquid

Additional notes

Assessed by densitometry.

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General info

Function

E2 ubiquitin-conjugating enzyme that accepts ubiquitin from an E1 ubiquitin-activating protein, and catalyzes its covalent attachment to other proteins by an E3 ubiquitin-protein ligase complex (PubMed : 12037680, PubMed : 20061386, PubMed : 38326650). In vitro catalyzes monoubiquitination and 'Lys-48'-linked polyubiquitination (PubMed : 12037680, PubMed : 20061386, PubMed : 38326650). Works in collaboration with various Cul1-RING and Cul2-RING E3 ligase complexes (PubMed : 38326650). May be involved in degradation of katenin (PubMed : 12037680).

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

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Product protocols

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Target data

E2 ubiquitin-conjugating enzyme that accepts ubiquitin from an E1 ubiquitin-activating protein, and catalyzes its covalent attachment to other proteins by an E3 ubiquitin-protein ligase complex (PubMed : 12037680, PubMed : 20061386, PubMed : 38326650). In vitro catalyzes monoubiquitination and 'Lys-48'-linked polyubiquitination (PubMed : 12037680, PubMed : 20061386, PubMed : 38326650). Works in collaboration with various Cul1-RING and Cul2-RING E3 ligase complexes (PubMed : 38326650). May be involved in degradation of katenin (PubMed : 12037680).
See full target information UBE2R2
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