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Recombinant human UBCH6/UBE2E1 protein (Active) is a Human Full Length protein, in the 2 to 193 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
Alternative names=Ubiquitin-conjugating enzyme E2 E1, (E3-independent) E2 ubiquitin-conjugating enzyme E1, E2 ubiquitin-conjugating enzyme E1, UbcH6, Ubiquitin carrier protein E1, Ubiquitin-protein ligase E1, UBCH6, UBE2E1
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Sequence
S D D D S R A S T S S S S S S S S N Q Q T E K E T N T P K K K E S K V S M S K N S K L L S T S A K R I Q K E L A D I T L D P P P N C S A G P K G D N I Y E W R S T I L G P P G S V Y E G G V F F L D I T F T P E Y P F K P P K V T F R T R I Y H C N I N S Q G V I C L D I L K D N W S P A L T I S K V L L S I C S L L T D C N P A D P L V G S I A T Q Y M T N R A E H D R M A R Q W T K R Y A T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant human UBCH6/UBE2E1 protein (Active) is a Human Full Length protein, in the 2 to 193 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
Alternative names=Ubiquitin-conjugating enzyme E2 E1, (E3-independent) E2 ubiquitin-conjugating enzyme E1, E2 ubiquitin-conjugating enzyme E1, UbcH6, Ubiquitin carrier protein E1, Ubiquitin-protein ligase E1, UBCH6, UBE2E1
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
The specific activity of ab269099 was 3 nmol/min/mg in a ubiquinating assay using wild-type ubiquitin as substrate.
- Amino acids
2 to 193
- Sequence
S D D D S R A S T S S S S S S S S N Q Q T E K E T N T P K K K E S K V S M S K N S K L L S T S A K R I Q K E L A D I T L D P P P N C S A G P K G D N I Y E W R S T I L G P P G S V Y E G G V F F L D I T F T P E Y P F K P P K V T F R T R I Y H C N I N S Q G V I C L D I L K D N W S P A L T I S K V L L S I C S L L T D C N P A D P L V G S I A T Q Y M T N R A E H D R M A R Q W T K R Y A T
- Accession
- P51965-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Specifications
- Form
Liquid
General info
- Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination.
- Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
- Post-translational modifications
ISGylation suppresses ubiquitin E2 enzyme activity.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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