Recombinant human Ube1L protein (Active) is a Human Full Length protein, in the 1 to 1012 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M D A L D A S K L L D E E L Y S R Q L Y V L G S P A M Q R I Q G A R V L V S G L Q G L G A E V A K N L V L M G V G S L T L H D P H P T C W S D L A A Q F L L S E Q D L E R S R A E A S Q E L L A Q L N R A V Q V V V H T G D I T E D L L L D F Q V V V L T A A K L E E Q L K V G T L C H K H G V C F L A A D T R G L V G Q L F C D F G E D F T V Q D P T E A E P L T A A I Q H I S Q G S P G I L T L R K G A N T H Y F R D G D L V T F S G I E G M V E L N D C D P R S I H V R E D G S L E I G D T T T F S R Y L R G G A I T E V K R P K T V R H K S L D T A L L Q P H V V A Q S S Q E V H H A H C L H Q A F C A L H K F Q H L H G R P P Q P W D P V D A E T V V G L A R D L E P L K R T E E E P L E E P L D E A L V R T V A L S S A G V L S P M V A M L G A V A A Q E V L K A I S R K F M P L D Q W L Y F D A L D C L P E D G E L L P S P E D C A L R G S R Y D G Q I A V F G A G F Q E K L R R Q H Y L L V G A G A I G C E L L K V F A L V G L G A G N S G G L T V V D M D H I E R S N L S R Q F L F R S Q D V G R P K A E V A A A A A R G L N P D L Q V I P L T Y P L D P T T E H I Y G D N F F S R V D G V A A A L D S F Q A R R Y V A A R C T H Y L K P L L E A G T S G T W G S A T V F M P H V T E A Y R A P A S A A A S E D A P Y P V C T V R Y F P S T A E H T L Q W A R H E F E E L F R L S A E T I N H H Q Q A H T S L A D M D E P Q T L T L L K P V L G V L R V R P Q N W Q D C V A W A L G H W K L C F H Y G I K Q L L R H F P P N K V L E D G T P F W S G P K Q C P Q P L E F D T N Q D T H L L Y V L A A A N L Y A Q M H G L P G S Q D W T A L R E L L K L L P Q P D P Q Q M A P I F A S N L E L A S A S A E F G P E Q Q K E L N K A L E V W S V G P P L K P L M F E K D D D S N F H V D F V V A A A S L R C Q N Y G I P P V N R A Q S K R I V G Q I I P A I A T T T A A V A G L L G L E L Y K V V S G P R P R S A F R H S Y L H L A E N Y L I R Y M P F A P A I Q T F H H L K W T S W D R L K V P A G Q P E R T L E S L L A H L Q E Q H G L R V R I L L H G S A L L Y A A G W S P E K Q A Q H L P L R V T E L V Q Q L T G Q A P A P G Q R V L V L E L S C E G D D E D T A F P P L H Y E L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
E1-activating enzyme that catalyzes the covalent conjugation of the ubiquitin-like protein product of ISG15 to additional interferon stimulated proteins (ISGs) as well as other cellular proteins such as P53 in a process termed protein ISGylation (PubMed:27545325). Plays an essential role in antiviral immunity together with ISG15 by restricting the replication of many viruses including rabies virus, influenza virus, sindbis virus, rotavirus or human cytomegalovirus (PubMed:16254333, PubMed:19073728, PubMed:29056542, PubMed:29743376, PubMed:37722521). For example, ISG15 modification of influenza A protein NS1 disrupts the association of the NS1 with importin-alpha leading to NS1 nuclear import inhibition (PubMed:20133869). ISGylation of human cytomegalovirs protein UL26 regulates its stability and inhibits its activities to suppress NF-kappa-B signaling (PubMed:27564865).
Alternative names
UBE1L, UBE2, UBA7, Ubiquitin-like modifier-activating enzyme 7, Ubiquitin-activating enzyme 7, D8, Ubiquitin-activating enzyme E1 homolog
Recommended products
Recombinant human Ube1L protein (Active) is a Human Full Length protein, in the 1 to 1012 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab269093 was 50 nmol/min/mg in a sumoylation assay using human ISG15 protein as substrate.
- Accession
- P41226-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M D A L D A S K L L D E E L Y S R Q L Y V L G S P A M Q R I Q G A R V L V S G L Q G L G A E V A K N L V L M G V G S L T L H D P H P T C W S D L A A Q F L L S E Q D L E R S R A E A S Q E L L A Q L N R A V Q V V V H T G D I T E D L L L D F Q V V V L T A A K L E E Q L K V G T L C H K H G V C F L A A D T R G L V G Q L F C D F G E D F T V Q D P T E A E P L T A A I Q H I S Q G S P G I L T L R K G A N T H Y F R D G D L V T F S G I E G M V E L N D C D P R S I H V R E D G S L E I G D T T T F S R Y L R G G A I T E V K R P K T V R H K S L D T A L L Q P H V V A Q S S Q E V H H A H C L H Q A F C A L H K F Q H L H G R P P Q P W D P V D A E T V V G L A R D L E P L K R T E E E P L E E P L D E A L V R T V A L S S A G V L S P M V A M L G A V A A Q E V L K A I S R K F M P L D Q W L Y F D A L D C L P E D G E L L P S P E D C A L R G S R Y D G Q I A V F G A G F Q E K L R R Q H Y L L V G A G A I G C E L L K V F A L V G L G A G N S G G L T V V D M D H I E R S N L S R Q F L F R S Q D V G R P K A E V A A A A A R G L N P D L Q V I P L T Y P L D P T T E H I Y G D N F F S R V D G V A A A L D S F Q A R R Y V A A R C T H Y L K P L L E A G T S G T W G S A T V F M P H V T E A Y R A P A S A A A S E D A P Y P V C T V R Y F P S T A E H T L Q W A R H E F E E L F R L S A E T I N H H Q Q A H T S L A D M D E P Q T L T L L K P V L G V L R V R P Q N W Q D C V A W A L G H W K L C F H Y G I K Q L L R H F P P N K V L E D G T P F W S G P K Q C P Q P L E F D T N Q D T H L L Y V L A A A N L Y A Q M H G L P G S Q D W T A L R E L L K L L P Q P D P Q Q M A P I F A S N L E L A S A S A E F G P E Q Q K E L N K A L E V W S V G P P L K P L M F E K D D D S N F H V D F V V A A A S L R C Q N Y G I P P V N R A Q S K R I V G Q I I P A I A T T T A A V A G L L G L E L Y K V V S G P R P R S A F R H S Y L H L A E N Y L I R Y M P F A P A I Q T F H H L K W T S W D R L K V P A G Q P E R T L E S L L A H L Q E Q H G L R V R I L L H G S A L L Y A A G W S P E K Q A Q H L P L R V T E L V Q Q L T G Q A P A P G Q R V L V L E L S C E G D D E D T A F P P L H Y E L
- Accession
- P41226
- Protein length
- Full Length
- Amino acids
- 1 to 1012
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
E1-activating enzyme that catalyzes the covalent conjugation of the ubiquitin-like protein product of ISG15 to additional interferon stimulated proteins (ISGs) as well as other cellular proteins such as P53 in a process termed protein ISGylation (PubMed:27545325). Plays an essential role in antiviral immunity together with ISG15 by restricting the replication of many viruses including rabies virus, influenza virus, sindbis virus, rotavirus or human cytomegalovirus (PubMed:16254333, PubMed:19073728, PubMed:29056542, PubMed:29743376, PubMed:37722521). For example, ISG15 modification of influenza A protein NS1 disrupts the association of the NS1 with importin-alpha leading to NS1 nuclear import inhibition (PubMed:20133869). ISGylation of human cytomegalovirs protein UL26 regulates its stability and inhibits its activities to suppress NF-kappa-B signaling (PubMed:27564865).
- Sequence similarities
Belongs to the ubiquitin-activating E1 family.
- Post-translational modifications
ISGylated.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ube1L also known as ubiquitin-activating enzyme E1-like plays an important role in the ubiquitin-proteasome system by activating ubiquitin molecules. With a molecular mass of approximately 117 kDa Ube1L functions as the initial enzyme in the ubiquitination cascade catalyzing the formation of a thioester bond with ubiquitin. This process is important for the tagging of proteins for degradation. Ube1L exhibits widespread expression in various tissues indicating its importance in diverse cellular activities.
- Biological function summary
This protein participates actively in the regulation of protein homeostasis. Ube1L helps to manage protein turnover and maintains protein quality control by marking damaged or unneeded proteins for degradation via the proteasome. Although Ube1L functions primarily as a standalone enzyme it cooperates closely with ubiquitin-conjugating and -ligating enzymes (E2s and E3s) to achieve precise ubiquitination of target proteins. This cooperation is critical for cellular processes like cell cycle regulation and response to stress.
- Pathways
Ube1L is a significant element in the ubiquitin-proteasome pathway which is essential for protein degradation and turnover. It also contributes to the regulation of interferon signaling by modifying proteins such as ISG15 a ubiquitin-like protein involved in antiviral response. Related proteins such as UBE2L6 and UBE2E1 serve as E2 conjugating enzymes that interact with Ube1L further influencing these pathways by facilitating the transfer of activated ubiquitin.
- Associated diseases and disorders
Ube1L has been implicated in certain types of cancers and viral infections. Alterations in the expression or function of Ube1L can lead to disrupted protein degradation contributing to cancer progression through the accumulation of oncogenic proteins. Ube1L is also connected to proteins like ISG15 and USP18 which are involved in host defense mechanisms against viral infections. Dysregulation in these contexts can result in inadequate antiviral responses or improper regulation of cell proliferation.
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2 product images
Functional Studies - Recombinant human Ube1L protein (Active) (ab269093)
The specific activity of ab269093 was 50 nmol/min/mg in a sumoylation assay using human ISG15 protein as substrate.
SDS-PAGE - Recombinant human Ube1L protein (Active) (ab269093)
SDS-PAGE analysis of ab269093.
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