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Recombinant human Ube1L protein (Active) is a Human Full Length protein, in the 1 to 1012 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.

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Images

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Amino acid sequence

M D A L D A S K L L D E E L Y S R Q L Y V L G S P A M Q R I Q G A R V L V S G L Q G L G A E V A K N L V L M G V G S L T L H D P H P T C W S D L A A Q F L L S E Q D L E R S R A E A S Q E L L A Q L N R A V Q V V V H T G D I T E D L L L D F Q V V V L T A A K L E E Q L K V G T L C H K H G V C F L A A D T R G L V G Q L F C D F G E D F T V Q D P T E A E P L T A A I Q H I S Q G S P G I L T L R K G A N T H Y F R D G D L V T F S G I E G M V E L N D C D P R S I H V R E D G S L E I G D T T T F S R Y L R G G A I T E V K R P K T V R H K S L D T A L L Q P H V V A Q S S Q E V H H A H C L H Q A F C A L H K F Q H L H G R P P Q P W D P V D A E T V V G L A R D L E P L K R T E E E P L E E P L D E A L V R T V A L S S A G V L S P M V A M L G A V A A Q E V L K A I S R K F M P L D Q W L Y F D A L D C L P E D G E L L P S P E D C A L R G S R Y D G Q I A V F G A G F Q E K L R R Q H Y L L V G A G A I G C E L L K V F A L V G L G A G N S G G L T V V D M D H I E R S N L S R Q F L F R S Q D V G R P K A E V A A A A A R G L N P D L Q V I P L T Y P L D P T T E H I Y G D N F F S R V D G V A A A L D S F Q A R R Y V A A R C T H Y L K P L L E A G T S G T W G S A T V F M P H V T E A Y R A P A S A A A S E D A P Y P V C T V R Y F P S T A E H T L Q W A R H E F E E L F R L S A E T I N H H Q Q A H T S L A D M D E P Q T L T L L K P V L G V L R V R P Q N W Q D C V A W A L G H W K L C F H Y G I K Q L L R H F P P N K V L E D G T P F W S G P K Q C P Q P L E F D T N Q D T H L L Y V L A A A N L Y A Q M H G L P G S Q D W T A L R E L L K L L P Q P D P Q Q M A P I F A S N L E L A S A S A E F G P E Q Q K E L N K A L E V W S V G P P L K P L M F E K D D D S N F H V D F V V A A A S L R C Q N Y G I P P V N R A Q S K R I V G Q I I P A I A T T T A A V A G L L G L E L Y K V V S G P R P R S A F R H S Y L H L A E N Y L I R Y M P F A P A I Q T F H H L K W T S W D R L K V P A G Q P E R T L E S L L A H L Q E Q H G L R V R I L L H G S A L L Y A A G W S P E K Q A Q H L P L R V T E L V Q Q L T G Q A P A P G Q R V L V L E L S C E G D D E D T A F P P L H Y E L

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Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.

Alternative names

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Recombinant human Ube1L protein (Active) is a Human Full Length protein, in the 1 to 1012 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.

Alternative names

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Applications

SDS-PAGE, FuncS

Biological activity

The specific activity of ab269093 was 50 nmol/min/mg in a sumoylation assay using human ISG15 protein as substrate.

Accession
P41226-1
Animal free

No

Species

Human

Concentration
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Storage buffer

pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Sequence info

Amino acid sequence

M D A L D A S K L L D E E L Y S R Q L Y V L G S P A M Q R I Q G A R V L V S G L Q G L G A E V A K N L V L M G V G S L T L H D P H P T C W S D L A A Q F L L S E Q D L E R S R A E A S Q E L L A Q L N R A V Q V V V H T G D I T E D L L L D F Q V V V L T A A K L E E Q L K V G T L C H K H G V C F L A A D T R G L V G Q L F C D F G E D F T V Q D P T E A E P L T A A I Q H I S Q G S P G I L T L R K G A N T H Y F R D G D L V T F S G I E G M V E L N D C D P R S I H V R E D G S L E I G D T T T F S R Y L R G G A I T E V K R P K T V R H K S L D T A L L Q P H V V A Q S S Q E V H H A H C L H Q A F C A L H K F Q H L H G R P P Q P W D P V D A E T V V G L A R D L E P L K R T E E E P L E E P L D E A L V R T V A L S S A G V L S P M V A M L G A V A A Q E V L K A I S R K F M P L D Q W L Y F D A L D C L P E D G E L L P S P E D C A L R G S R Y D G Q I A V F G A G F Q E K L R R Q H Y L L V G A G A I G C E L L K V F A L V G L G A G N S G G L T V V D M D H I E R S N L S R Q F L F R S Q D V G R P K A E V A A A A A R G L N P D L Q V I P L T Y P L D P T T E H I Y G D N F F S R V D G V A A A L D S F Q A R R Y V A A R C T H Y L K P L L E A G T S G T W G S A T V F M P H V T E A Y R A P A S A A A S E D A P Y P V C T V R Y F P S T A E H T L Q W A R H E F E E L F R L S A E T I N H H Q Q A H T S L A D M D E P Q T L T L L K P V L G V L R V R P Q N W Q D C V A W A L G H W K L C F H Y G I K Q L L R H F P P N K V L E D G T P F W S G P K Q C P Q P L E F D T N Q D T H L L Y V L A A A N L Y A Q M H G L P G S Q D W T A L R E L L K L L P Q P D P Q Q M A P I F A S N L E L A S A S A E F G P E Q Q K E L N K A L E V W S V G P P L K P L M F E K D D D S N F H V D F V V A A A S L R C Q N Y G I P P V N R A Q S K R I V G Q I I P A I A T T T A A V A G L L G L E L Y K V V S G P R P R S A F R H S Y L H L A E N Y L I R Y M P F A P A I Q T F H H L K W T S W D R L K V P A G Q P E R T L E S L L A H L Q E Q H G L R V R I L L H G S A L L Y A A G W S P E K Q A Q H L P L R V T E L V Q Q L T G Q A P A P G Q R V L V L E L S C E G D D E D T A F P P L H Y E L

Accession

P41226

Protein length

Full Length

Amino acids

1 to 1012

Nature

Recombinant

Tags

GST tag N-Terminus

Specifications

Form

Liquid

Additional notes

Affinity purified.

General info

Function

Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.

Sequence similarities

Belongs to the ubiquitin-activating E1 family.

Post-translational modifications

ISGylated.

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

Biological function summary

This protein participates actively in the regulation of protein homeostasis. Ube1L helps to manage protein turnover and maintains protein quality control by marking damaged or unneeded proteins for degradation via the proteasome. Although Ube1L functions primarily as a standalone enzyme it cooperates closely with ubiquitin-conjugating and -ligating enzymes (E2s and E3s) to achieve precise ubiquitination of target proteins. This cooperation is critical for cellular processes like cell cycle regulation and response to stress.

Activity summary

Ube1L also known as ubiquitin-activating enzyme E1-like plays an important role in the ubiquitin-proteasome system by activating ubiquitin molecules. With a molecular mass of approximately 117 kDa Ube1L functions as the initial enzyme in the ubiquitination cascade catalyzing the formation of a thioester bond with ubiquitin. This process is important for the tagging of proteins for degradation. Ube1L exhibits widespread expression in various tissues indicating its importance in diverse cellular activities.

Pathways

Ube1L is a significant element in the ubiquitin-proteasome pathway which is essential for protein degradation and turnover. It also contributes to the regulation of interferon signaling by modifying proteins such as ISG15 a ubiquitin-like protein involved in antiviral response. Related proteins such as UBE2L6 and UBE2E1 serve as E2 conjugating enzymes that interact with Ube1L further influencing these pathways by facilitating the transfer of activated ubiquitin.

Associated diseases and disorders

Ube1L has been implicated in certain types of cancers and viral infections. Alterations in the expression or function of Ube1L can lead to disrupted protein degradation contributing to cancer progression through the accumulation of oncogenic proteins. Ube1L is also connected to proteins like ISG15 and USP18 which are involved in host defense mechanisms against viral infections. Dysregulation in these contexts can result in inadequate antiviral responses or improper regulation of cell proliferation.

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2 product images

  • Functional Studies - Recombinant human Ube1L protein (Active) (ab269093), expandable thumbnail

    Functional Studies - Recombinant human Ube1L protein (Active) (ab269093)

    The specific activity of ab269093 was 50 nmol/min/mg in a sumoylation assay using human ISG15 protein as substrate.

  • SDS-PAGE - Recombinant human Ube1L protein (Active) (ab269093), expandable thumbnail

    SDS-PAGE - Recombinant human Ube1L protein (Active) (ab269093)

    SDS-PAGE analysis of ab269093.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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