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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Recombinant human Ube1L protein (Active) is a Human Full Length protein, in the 1 to 1012 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
>95% SDS-PAGE
Baculovirus infected Sf9 cells
GST tag N-Terminus
SDS-PAGE, FuncS
Yes
M D A L D A S K L L D E E L Y S R Q L Y V L G S P A M Q R I Q G A R V L V S G L Q G L G A E V A K N L V L M G V G S L T L H D P H P T C W S D L A A Q F L L S E Q D L E R S R A E A S Q E L L A Q L N R A V Q V V V H T G D I T E D L L L D F Q V V V L T A A K L E E Q L K V G T L C H K H G V C F L A A D T R G L V G Q L F C D F G E D F T V Q D P T E A E P L T A A I Q H I S Q G S P G I L T L R K G A N T H Y F R D G D L V T F S G I E G M V E L N D C D P R S I H V R E D G S L E I G D T T T F S R Y L R G G A I T E V K R P K T V R H K S L D T A L L Q P H V V A Q S S Q E V H H A H C L H Q A F C A L H K F Q H L H G R P P Q P W D P V D A E T V V G L A R D L E P L K R T E E E P L E E P L D E A L V R T V A L S S A G V L S P M V A M L G A V A A Q E V L K A I S R K F M P L D Q W L Y F D A L D C L P E D G E L L P S P E D C A L R G S R Y D G Q I A V F G A G F Q E K L R R Q H Y L L V G A G A I G C E L L K V F A L V G L G A G N S G G L T V V D M D H I E R S N L S R Q F L F R S Q D V G R P K A E V A A A A A R G L N P D L Q V I P L T Y P L D P T T E H I Y G D N F F S R V D G V A A A L D S F Q A R R Y V A A R C T H Y L K P L L E A G T S G T W G S A T V F M P H V T E A Y R A P A S A A A S E D A P Y P V C T V R Y F P S T A E H T L Q W A R H E F E E L F R L S A E T I N H H Q Q A H T S L A D M D E P Q T L T L L K P V L G V L R V R P Q N W Q D C V A W A L G H W K L C F H Y G I K Q L L R H F P P N K V L E D G T P F W S G P K Q C P Q P L E F D T N Q D T H L L Y V L A A A N L Y A Q M H G L P G S Q D W T A L R E L L K L L P Q P D P Q Q M A P I F A S N L E L A S A S A E F G P E Q Q K E L N K A L E V W S V G P P L K P L M F E K D D D S N F H V D F V V A A A S L R C Q N Y G I P P V N R A Q S K R I V G Q I I P A I A T T T A A V A G L L G L E L Y K V V S G P R P R S A F R H S Y L H L A E N Y L I R Y M P F A P A I Q T F H H L K W T S W D R L K V P A G Q P E R T L E S L L A H L Q E Q H G L R V R I L L H G S A L L Y A A G W S P E K Q A Q H L P L R V T E L V Q Q L T G Q A P A P G Q R V L V L E L S C E G D D E D T A F P P L H Y E L
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.
Ubiquitin-like modifier-activating enzyme 7, Ubiquitin-activating enzyme 7, D8, Ubiquitin-activating enzyme E1 homolog, UBA7, UBE1L, UBE2
Recombinant human Ube1L protein (Active) is a Human Full Length protein, in the 1 to 1012 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
Ubiquitin-like modifier-activating enzyme 7, Ubiquitin-activating enzyme 7, D8, Ubiquitin-activating enzyme E1 homolog, UBA7, UBE1L, UBE2
>95% SDS-PAGE
Baculovirus infected Sf9 cells
GST tag N-Terminus
SDS-PAGE, FuncS
Yes
The specific activity of ab269093 was 50 nmol/min/mg in a sumoylation assay using human ISG15 protein as substrate.
No
Human
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
M D A L D A S K L L D E E L Y S R Q L Y V L G S P A M Q R I Q G A R V L V S G L Q G L G A E V A K N L V L M G V G S L T L H D P H P T C W S D L A A Q F L L S E Q D L E R S R A E A S Q E L L A Q L N R A V Q V V V H T G D I T E D L L L D F Q V V V L T A A K L E E Q L K V G T L C H K H G V C F L A A D T R G L V G Q L F C D F G E D F T V Q D P T E A E P L T A A I Q H I S Q G S P G I L T L R K G A N T H Y F R D G D L V T F S G I E G M V E L N D C D P R S I H V R E D G S L E I G D T T T F S R Y L R G G A I T E V K R P K T V R H K S L D T A L L Q P H V V A Q S S Q E V H H A H C L H Q A F C A L H K F Q H L H G R P P Q P W D P V D A E T V V G L A R D L E P L K R T E E E P L E E P L D E A L V R T V A L S S A G V L S P M V A M L G A V A A Q E V L K A I S R K F M P L D Q W L Y F D A L D C L P E D G E L L P S P E D C A L R G S R Y D G Q I A V F G A G F Q E K L R R Q H Y L L V G A G A I G C E L L K V F A L V G L G A G N S G G L T V V D M D H I E R S N L S R Q F L F R S Q D V G R P K A E V A A A A A R G L N P D L Q V I P L T Y P L D P T T E H I Y G D N F F S R V D G V A A A L D S F Q A R R Y V A A R C T H Y L K P L L E A G T S G T W G S A T V F M P H V T E A Y R A P A S A A A S E D A P Y P V C T V R Y F P S T A E H T L Q W A R H E F E E L F R L S A E T I N H H Q Q A H T S L A D M D E P Q T L T L L K P V L G V L R V R P Q N W Q D C V A W A L G H W K L C F H Y G I K Q L L R H F P P N K V L E D G T P F W S G P K Q C P Q P L E F D T N Q D T H L L Y V L A A A N L Y A Q M H G L P G S Q D W T A L R E L L K L L P Q P D P Q Q M A P I F A S N L E L A S A S A E F G P E Q Q K E L N K A L E V W S V G P P L K P L M F E K D D D S N F H V D F V V A A A S L R C Q N Y G I P P V N R A Q S K R I V G Q I I P A I A T T T A A V A G L L G L E L Y K V V S G P R P R S A F R H S Y L H L A E N Y L I R Y M P F A P A I Q T F H H L K W T S W D R L K V P A G Q P E R T L E S L L A H L Q E Q H G L R V R I L L H G S A L L Y A A G W S P E K Q A Q H L P L R V T E L V Q Q L T G Q A P A P G Q R V L V L E L S C E G D D E D T A F P P L H Y E L
Full Length
1 to 1012
Recombinant
GST tag N-Terminus
Liquid
Affinity purified.
Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.
Belongs to the ubiquitin-activating E1 family.
ISGylated.
Dry Ice
-80°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
This protein participates actively in the regulation of protein homeostasis. Ube1L helps to manage protein turnover and maintains protein quality control by marking damaged or unneeded proteins for degradation via the proteasome. Although Ube1L functions primarily as a standalone enzyme it cooperates closely with ubiquitin-conjugating and -ligating enzymes (E2s and E3s) to achieve precise ubiquitination of target proteins. This cooperation is critical for cellular processes like cell cycle regulation and response to stress.
Ube1L also known as ubiquitin-activating enzyme E1-like plays an important role in the ubiquitin-proteasome system by activating ubiquitin molecules. With a molecular mass of approximately 117 kDa Ube1L functions as the initial enzyme in the ubiquitination cascade catalyzing the formation of a thioester bond with ubiquitin. This process is important for the tagging of proteins for degradation. Ube1L exhibits widespread expression in various tissues indicating its importance in diverse cellular activities.
Ube1L is a significant element in the ubiquitin-proteasome pathway which is essential for protein degradation and turnover. It also contributes to the regulation of interferon signaling by modifying proteins such as ISG15 a ubiquitin-like protein involved in antiviral response. Related proteins such as UBE2L6 and UBE2E1 serve as E2 conjugating enzymes that interact with Ube1L further influencing these pathways by facilitating the transfer of activated ubiquitin.
Ube1L has been implicated in certain types of cancers and viral infections. Alterations in the expression or function of Ube1L can lead to disrupted protein degradation contributing to cancer progression through the accumulation of oncogenic proteins. Ube1L is also connected to proteins like ISG15 and USP18 which are involved in host defense mechanisms against viral infections. Dysregulation in these contexts can result in inadequate antiviral responses or improper regulation of cell proliferation.
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Terms & Conditions.
The specific activity of ab269093 was 50 nmol/min/mg in a sumoylation assay using human ISG15 protein as substrate.
SDS-PAGE analysis of ab269093.
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