Recombinant Human UBE2D3 protein (His-tag) is a Human Full Length protein, expressed in HEK 293, with >=95% purity, <= 1.83 EU/mg endotoxin level and suitable for HPLC, MS.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 1.83 EU/mg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- HPLC, MS
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
Amino acid sequence
M A L K R I N K E L S D L A R D P P A Q C S A G P V G D D M F H W Q A T I M G P N D S P Y Q G G V F F L T I H F P T D Y P F K P P K V A F T T R I Y H P N I N S N G S I C L D I L R S Q W S P A L T I S K V L L S I C S L L C D P N P D D P L V P E I A R I Y K T D R D K Y N R I S R E W T Q K Y A M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:15247280, PubMed:15496420, PubMed:18284575, PubMed:20061386, PubMed:21532592, PubMed:28322253). In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination (PubMed:15247280, PubMed:15496420, PubMed:18284575, PubMed:20061386, PubMed:21532592). Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation (PubMed:20347421). Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin (PubMed:10329681). Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin (PubMed:10329681). Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA (PubMed:18948756). Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase (PubMed:18948756). Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair (PubMed:16628214). Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML-NB) formation in the nucleus (PubMed:18515077). In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53 (PubMed:12646252, PubMed:15280377). In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes (PubMed:18508924). In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction (PubMed:11743028). Together with RNF135, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production (PubMed:28469175). Together with ZNF598, catalyzes ubiquitination of 40S ribosomal proteins in response to ribosome collisions (PubMed:28685749). In cooperation with the GATOR2 complex, catalyzes 'Lys-6'-linked ubiquitination of NPRL2 (PubMed:36528027).
Alternative names
UBC5C, UBCH5C, UBE2D3, Ubiquitin-conjugating enzyme E2 D3, (E3-independent) E2 ubiquitin-conjugating enzyme D3, E2 ubiquitin-conjugating enzyme D3, Ubiquitin carrier protein D3, Ubiquitin-conjugating enzyme E2(17)KB 3, Ubiquitin-conjugating enzyme E2-17 kDa 3, Ubiquitin-protein ligase D3
Recommended products
Recombinant Human UBE2D3 protein (His-tag) is a Human Full Length protein, expressed in HEK 293, with >=95% purity, <= 1.83 EU/mg endotoxin level and suitable for HPLC, MS.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 1.83 EU/mg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- HPLC, MS
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
- Accession
- P61077-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- M A L K R I N K E L S D L A R D P P A Q C S A G P V G D D M F H W Q A T I M G P N D S P Y Q G G V F F L T I H F P T D Y P F K P P K V A F T T R I Y H P N I N S N G S I C L D I L R S Q W S P A L T I S K V L L S I C S L L C D P N P D D P L V P E I A R I Y K T D R D K Y N R I S R E W T Q K Y A M
- Accession
- P61077
- Protein length
- Full Length
- Predicted molecular weight
- 18.87 kDa
- Actual molecular weight
- 18.87 kDa
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:15247280, PubMed:15496420, PubMed:18284575, PubMed:20061386, PubMed:21532592, PubMed:28322253). In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination (PubMed:15247280, PubMed:15496420, PubMed:18284575, PubMed:20061386, PubMed:21532592). Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation (PubMed:20347421). Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin (PubMed:10329681). Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin (PubMed:10329681). Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA (PubMed:18948756). Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase (PubMed:18948756). Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair (PubMed:16628214). Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML-NB) formation in the nucleus (PubMed:18515077). In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53 (PubMed:12646252, PubMed:15280377). In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes (PubMed:18508924). In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction (PubMed:11743028). Together with RNF135, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production (PubMed:28469175). Together with ZNF598, catalyzes ubiquitination of 40S ribosomal proteins in response to ribosome collisions (PubMed:28685749). In cooperation with the GATOR2 complex, catalyzes 'Lys-6'-linked ubiquitination of NPRL2 (PubMed:36528027).
- Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
- Post-translational modifications
Phosphorylated by AURKB.
- Subcellular localisation
- Endosome membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UBE2D3 also known as UbcH5c or E2-17k is a ubiquitin-conjugating enzyme. It plays a critical role in the ubiquitination process by interacting with ligase enzymes adding ubiquitin to target proteins. This protein has a molecular mass of approximately 16 kDa. UBE2D3 is widely expressed in various tissues indicating its role in several cellular functions.
- Biological function summary
UBE2D3 acts as an important component in protein degradation and regulation. It participates in the ubiquitin-proteasome system which controls protein levels in cells. UBE2D3 forms part of larger protein complexes such as the SCF (Skp Cullin F-box containing) complex contributing to the regulation of cell cycle and signal transduction. This interaction helps control cell division and response to external signals.
- Pathways
UBE2D3 plays an important role in the NF-kB signaling and DNA damage response pathways. It interacts with proteins such as IKK (IκB kinase) to regulate inflammation and immune response. Additionally UBE2D3's involvement in the DNA repair pathway supports maintaining genome stability by collaborating with other proteins like BRCA1 which is essential for repairing DNA double-strand breaks.
- Associated diseases and disorders
UBE2D3 shows a connection to cancer and neurodegenerative diseases. Its role in the NF-kB signaling pathway links it to cancer development due to its regulation of genes involved in cell growth and survival. In neurodegenerative disorders such as Alzheimer's disease abnormal ubiquitination due to UBE2D3 dysfunction may lead to protein aggregation affecting normal cellular activities. Its interaction with proteins like p53 a well-known tumor suppressor illustrates its importance in disease progression and regulation.
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2 product images
Mass Spectrometry - Recombinant Human UBE2D3 protein (His-tag) (ab317878)
Mass determination by ESI-TOF. Predicted MW is 18870 Da (+/- 10 Da by ESI-TOF). Observed MW is 18866.46 Da.
HPLC - Recombinant Human UBE2D3 protein (His-tag) (ab317878)
HPLC analysis of ab317878
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