Recombinant Human Ube2H protein is a Human Full Length protein, in the 1 to 183 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M S S P S P G K R R M D T D V V K L I E S K H E V T I L G G L N E F V V K F Y G P Q G T P Y E G G V W K V R V D L P D K Y P F K S P S I G F M N K I F H P N I D E A S G T V C L D V I N Q T W T A L Y D L T N I F E S F L P Q L L A Y P N P I D P L N G D A A A M Y L H R P E E Y K Q K I K E Y I Q K Y A T E E A L K E Q E E G T G D S S S E S S M S D F S E D E A Q D M E L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:17588522, PubMed:20061386, PubMed:8132613). E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex (PubMed:29911972). In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination (PubMed:20061386). Capable, in vitro, to ubiquitinate histone H2A (PubMed:8132613).
Alternative names
Ubiquitin-conjugating enzyme E2 H, (E3-independent) E2 ubiquitin-conjugating enzyme H, E2 ubiquitin-conjugating enzyme H, UbcH2, Ubiquitin carrier protein H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-protein ligase H, UBE2H
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Recombinant Human Ube2H protein is a Human Full Length protein, in the 1 to 183 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P62256-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.29% Sodium chloride, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M S S P S P G K R R M D T D V V K L I E S K H E V T I L G G L N E F V V K F Y G P Q G T P Y E G G V W K V R V D L P D K Y P F K S P S I G F M N K I F H P N I D E A S G T V C L D V I N Q T W T A L Y D L T N I F E S F L P Q L L A Y P N P I D P L N G D A A A M Y L H R P E E Y K Q K I K E Y I Q K Y A T E E A L K E Q E E G T G D S S S E S S M S D F S E D E A Q D M E L
- Accession
- P62256
- Protein length
- Full Length
- Predicted molecular weight
- 23.1 kDa
- Amino acids
- 1 to 183
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab124593 is purified using conventional chromatography techniques.
General info
- Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:17588522, PubMed:20061386, PubMed:8132613). E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex (PubMed:29911972). In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination (PubMed:20061386). Capable, in vitro, to ubiquitinate histone H2A (PubMed:8132613).
- Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
- Post-translational modifications
Autoubiquitinated in vitro in the presence of NEDD4L.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ubiquitin-conjugating enzyme E2 H (Ube2H) also known as UBC8 is an enzyme that functions mechanically to facilitate the transfer of ubiquitin to substrate proteins a process essential for regulating protein degradation. Ube2H weighs approximately 20 kDa and shows expression in various tissues especially in the liver and skeletal muscle. It belongs to the E2 ubiquitin-conjugating enzyme family which plays an important role in the ubiquitin-proteasome pathway.
- Biological function summary
Ubiquitin-conjugating enzyme E2 H plays a central role in protein homeostasis by tagging proteins for proteasomal degradation. It does not function alone but as part of larger complexes that include E1 activating enzymes and E3 ubiquitin ligases. These complexes work in synchrony to ensure precise ubiquitination of target proteins which helps regulate multiple cellular processes such as cell cycle progression DNA repair and immune response.
- Pathways
Ubiquitin-conjugating enzyme E2 H integrates with several critical cellular pathways including the ubiquitin-proteasome pathway and the DNA damage response pathway. In the ubiquitin-proteasome pathway Ube2H works alongside other E2 enzymes like Ube2C to assist in the ubiquitination process. It has a notable connection with proteins like p53 and BRCA1 which are vital in maintaining genomic stability and tumor suppression.
- Associated diseases and disorders
Ubiquitin-conjugating enzyme E2 H has recognized implications in cancer and neurodegenerative diseases. Aberrations in its function can lead to improper protein degradation contributing to the accumulation of defective proteins that can cause cellular dysfunction. In cancer Ube2H is often linked with anomalies in the ubiquitin-proteasome system affecting the degradation of oncogenic proteins. Additionally its interaction with p53 a protein known for its tumor suppressor functions highlights its potential involvement in cancer progression when dysregulated.
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1 product image
SDS-PAGE - Recombinant Human Ube2H protein (ab124593)
15% SDS-PAGE showing ab124593 (3μg).
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