Recombinant human UBE2I / UBC9 protein (Active)
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Recombinant human UBE2I / UBC9 protein (Active) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.
View Alternative Names
UBC9, UBCE9, UBE2I, SUMO-conjugating enzyme UBC9, RING-type E3 SUMO transferase UBC9, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18
- FuncS
Supplier Data
Functional Studies - Recombinant human UBE2I / UBC9 protein (Active) (AB269103)
The specific activity of ab269103 was 200 nmol/min/mg in a UBE2I assay using human SUMO2 and RanGAP1 as substrate.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human UBE2I / UBC9 protein (Active) (AB269103)
SDS-PAGE analysis of ab269103.
Complementary Products
Reactivity data
Sequence info
Properties and storage information
Form
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The SUMO conjugating enzyme UBE2I is part of the SUMOylation complex which includes the SUMO E1 enzyme and SUMO E3 ligase. It facilitates the attachment of SUMO proteins to target proteins which can influence protein stability localization and interaction with other proteins. This modification usually regulates important cellular functions such as transcription DNA repair and cell cycle progression.
Pathways
Proteins modified by UBE2I participate in significant biological pathways such as the DNA damage response and cell cycle regulation. In the DNA damage response UBE2I works with proteins such as p53 and BRCA1 to maintain genomic stability. In cell cycle pathways interactions with proteins like cyclin D1 and CDK inhibitors help control cellular proliferation by SUMOylating key regulatory proteins.
General info
Function
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity (PubMed : 26620705). Sumoylates SHMT1 at 'Lys-38' or 'Lys-39' leading to RAN-dependent nuclear import of SHMT1 (PubMed : 17446168). Also sumoylates TYMS and DHFR (PubMed : 18067453).
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Post-translational modifications
Phosphorylation at Ser-71 significantly enhances SUMOylation activity.
Subcellular localisation
Nucleus
Target data
Product promise
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