Recombinant Human Ube2L3/UBCH7 protein
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Recombinant Human Ube2L3/UBCH7 protein is a Human Full Length protein, in the 1 to 154 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.
View Alternative Names
UBCE7, UBCH7, UBE2L3, Ubiquitin-conjugating enzyme E2 L3, E2 ubiquitin-conjugating enzyme L3, L-UBC, UbcH7, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human Ube2L3/UBCH7 protein (AB48775)
14% SDS-PAGE gel loaded with ab48775
recombinant human Ube2L3/UBCH7 protein
predicted molecular weight 17.9KDa
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Ube2L3 plays essential roles in protein turnover and cell cycle regulation by functioning within multi-protein complexes. It helps facilitate the attachment of ubiquitin to specific target proteins marking them for degradation. These activities are important for maintaining protein homeostasis and regulation of cell division apoptosis and signal transduction. Ube2L3's role in these biological processes makes it an important component of cellular function and physiology.
Pathways
Ube2L3 is involved in the ubiquitin-proteasome pathway and is also associated with the NF-kB signaling pathway. Its function in these pathways highlights its role in regulating immune responses and inflammatory processes. Interaction with other proteins like E3 ligases including Parkin and Mdm2 shows its involvement in protein quality control and cellular stress responses. Ube2L3's modulation of these pathways is significant for proper cellular responses to environmental cues and stressors.
Specifications
Form
Liquid
Additional notes
Ube2L3/UBCH7 was overexpressed in E.coli and purified by using conventional chromatography techniques.
General info
Function
Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine; in contrast, it has activity with the RBR family E3 enzymes, such as PRKN, RNF31 and ARIH1, that function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates ubiquitination by the CUL9-RBX1 complex (PubMed : 38605244). In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Post-translational modifications
Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation. Autoubiquitinated in vitro (PubMed:22496338).
Subcellular localisation
Nucleus
Target data
Complementary Products
Product promise
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