Recombinant human UBE2O protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M T V E Q L L T G S P T S P T V E P E K P T R E K K F L D D I K K L Q E N L K K T L D N V A I V E E E K M E A V P D V E R K E D K P E G Q S P V K A E W P S E T P V L C Q Q C G G K P G V T F T S A K G E V F S V L E F A P S N H S F K K I E F Q P P E A K K F F S T V R K E M A L L A T S L P E G I M V K T F E D R M D L F S A L I K G P T R T P Y E D G L Y L F D I Q L P N I Y P A V P P H F C Y L S Q C S G R L N P N L Y D N G K V C V S L L G T C I G K G T E R W T S K S S L L Q V L I S I Q G L I L V N E P Y Y N E A G F D S D R G L Q E G Y E N S R C Y N E M A L I R V V Q S M T Q L V R R P P E V F E Q E I R Q H F S T G G W R L V N R I E S W L E T H A L L E K A Q A L P N G V P K A S S S P E P P A V A E L S D S G Q Q E P E D G G P A P G E A S Q G S D S E G G A Q S L A S A S R D H T D Q T S E T A P D A S V P P S V K P K K R R K S Y R S F L P E K S G Y P D I G F P L F P L S K G F I K S I R G V L T Q F R A A L L E A G M P E C T E D K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853).
Alternative names
KIAA1734, UBE2O, (E3-independent) E2 ubiquitin-conjugating enzyme, E2/E3 hybrid ubiquitin-protein ligase UBE2O, Ubiquitin carrier protein O, Ubiquitin-conjugating enzyme E2 O, Ubiquitin-conjugating enzyme E2 of 230 kDa, Ubiquitin-protein ligase O, Ubiquitin-conjugating enzyme E2-230K
Recommended products
Recombinant human UBE2O protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of UBE20 was determined to be 25 nmol/min/mg in ubiquitination assay using wild-type ubiquitin protein substrate. We have not tested the E3 ligase activity.
- Accession
- Q9C0C9-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M T V E Q L L T G S P T S P T V E P E K P T R E K K F L D D I K K L Q E N L K K T L D N V A I V E E E K M E A V P D V E R K E D K P E G Q S P V K A E W P S E T P V L C Q Q C G G K P G V T F T S A K G E V F S V L E F A P S N H S F K K I E F Q P P E A K K F F S T V R K E M A L L A T S L P E G I M V K T F E D R M D L F S A L I K G P T R T P Y E D G L Y L F D I Q L P N I Y P A V P P H F C Y L S Q C S G R L N P N L Y D N G K V C V S L L G T C I G K G T E R W T S K S S L L Q V L I S I Q G L I L V N E P Y Y N E A G F D S D R G L Q E G Y E N S R C Y N E M A L I R V V Q S M T Q L V R R P P E V F E Q E I R Q H F S T G G W R L V N R I E S W L E T H A L L E K A Q A L P N G V P K A S S S P E P P A V A E L S D S G Q Q E P E D G G P A P G E A S Q G S D S E G G A Q S L A S A S R D H T D Q T S E T A P D A S V P P S V K P K K R R K S Y R S F L P E K S G Y P D I G F P L F P L S K G F I K S I R G V L T Q F R A A L L E A G M P E C T E D K
- Accession
- Q9C0C9
- Protein length
- Full Length
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853).
- Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
- Post-translational modifications
Phosphorylated. Phosphorylation affects subcellular location.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UBE2O also known as E2-230K or E2 ubiquitin-conjugating enzyme O is a protein that functions as an E2 ubiquitin-conjugating enzyme. It has a molecular mass of approximately 141 kDa. UBE2O plays a role in transferring ubiquitin to substrate proteins marking them for degradation via the proteasome. This process is important for protein quality control and cellular homeostasis. UBE2O is expressed in various tissues including the brain liver and skeletal muscle indicating its broad physiological significance.
- Biological function summary
UBE2O facilitates the regulation of protein turnover by mediating ubiquitination without the need for an E3 ligase. It acts both as an E2 and has E3 activity making it a versatile factor in the ubiquitin-proteasome system. UBE2O also participates in muscle differentiation by targeting specific muscle regulatory factors for ubiquitination. Understanding its function provides insights into the control of muscle mass and function highlighting its contribution to cellular growth and adaptation.
- Pathways
UBE2O interacts with essential biological mechanisms notably the ubiquitin-proteasome pathway involved in protein degradation. It also participates in cell cycle regulation by targeting certain cyclin proteins for degradation ensuring proper cell cycle progression. UBE2O works in conjunction with other proteins like ANAPC10 and CDC20 which are important in cell cycle control and proteostasis.
- Associated diseases and disorders
UBE2O has associations with conditions such as myopathies and certain cancers. In muscle disorders UBE2O dysregulation can lead to abnormal protein accumulation affecting muscle function. In oncology UBE2O interacts with proteins like P53 which is important in cell cycle regulation and apoptosis. Its role in these disorders underlines its importance as a potential therapeutic target for intervention in these diseases.
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2 product images
Functional Studies - Recombinant human UBE2O protein (Active) (ab269108)
Protein activity of ab269108 was determined to be 25 nmol/min/mg in an ubiquitination assay using wild-type ubiquitin protein substrate.
SDS-PAGE - Recombinant human UBE2O protein (Active) (ab269108)
SDS-PAGE analysis of ab269108.
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