Recombinant human UBE2O protein (Active)
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Recombinant human UBE2O protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.
View Alternative Names
KIAA1734, UBE2O, (E3-independent) E2 ubiquitin-conjugating enzyme, E2/E3 hybrid ubiquitin-protein ligase UBE2O, Ubiquitin carrier protein O, Ubiquitin-conjugating enzyme E2 O, Ubiquitin-conjugating enzyme E2 of 230 kDa, Ubiquitin-protein ligase O, Ubiquitin-conjugating enzyme E2-230K
- FuncS
Supplier Data
Functional Studies - Recombinant human UBE2O protein (Active) (AB269108)
Protein activity of ab269108 was determined to be 25 nmol/min/mg in an ubiquitination assay using wild-type ubiquitin protein substrate.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human UBE2O protein (Active) (AB269108)
SDS-PAGE analysis of ab269108.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
UBE2O facilitates the regulation of protein turnover by mediating ubiquitination without the need for an E3 ligase. It acts both as an E2 and has E3 activity making it a versatile factor in the ubiquitin-proteasome system. UBE2O also participates in muscle differentiation by targeting specific muscle regulatory factors for ubiquitination. Understanding its function provides insights into the control of muscle mass and function highlighting its contribution to cellular growth and adaptation.
Pathways
UBE2O interacts with essential biological mechanisms notably the ubiquitin-proteasome pathway involved in protein degradation. It also participates in cell cycle regulation by targeting certain cyclin proteins for degradation ensuring proper cell cycle progression. UBE2O works in conjunction with other proteins like ANAPC10 and CDC20 which are important in cell cycle control and proteostasis.
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed : 23455153, PubMed : 24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed : 23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed : 23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed : 24703950). Acts as a regulator of retrograde transport by assisting the TRIM27 : MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed : 23452853).
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Post-translational modifications
Phosphorylated. Phosphorylation affects subcellular location.. Ubiquitinated: autoubiquitinates, possibly affecting its subcellular location.
Subcellular localisation
Nucleus
Target data
Complementary Products
Product promise
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