Recombinant Human UBE2Q2 protein is a Human Full Length protein, in the 1 to 375 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M S V S G L K A E L K F L A S I F D K N H E R F R I V S W K L D E L H C Q F L V P Q Q G S P H S L P P P L T L H C N I T E S Y P S S S P I W F V D S E D P N L T S V L E R L E D T K N N N L L R Q Q L K W L I C E L C S L Y N L P K H L D V E M L D Q P L P T G Q N G T T E E V T S E E E E E E E E M A E D I E D L D H Y E M K E E E P I S G K K S E D E G I E K E N L A I L E K I R K T Q R Q D H L N G A V S G S V Q A S D R L M K E L R D I Y R S Q S Y K T G I Y S V E L I N D S L Y D W H V K L Q K V D P D S P L H S D L Q I L K E K E G I E Y I L L N F S F K D N F P F D P P F V R V V L P V L S G G Y V L G G G A L C M E L L T K Q G W S S A Y S I E S V I M Q I N A T L V K G K A R V Q F G A N K N Q Y N L A R A Q Q S Y N S I V Q I H E K N G W Y T P P K E D G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination.
Alternative names
Ubiquitin-conjugating enzyme E2 Q2, E2 ubiquitin-conjugating enzyme Q2, Ubiquitin carrier protein Q2, Ubiquitin-protein ligase Q2, UBE2Q2
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Recombinant Human UBE2Q2 protein is a Human Full Length protein, in the 1 to 375 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q8WVN8-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M S V S G L K A E L K F L A S I F D K N H E R F R I V S W K L D E L H C Q F L V P Q Q G S P H S L P P P L T L H C N I T E S Y P S S S P I W F V D S E D P N L T S V L E R L E D T K N N N L L R Q Q L K W L I C E L C S L Y N L P K H L D V E M L D Q P L P T G Q N G T T E E V T S E E E E E E E E M A E D I E D L D H Y E M K E E E P I S G K K S E D E G I E K E N L A I L E K I R K T Q R Q D H L N G A V S G S V Q A S D R L M K E L R D I Y R S Q S Y K T G I Y S V E L I N D S L Y D W H V K L Q K V D P D S P L H S D L Q I L K E K E G I E Y I L L N F S F K D N F P F D P P F V R V V L P V L S G G Y V L G G G A L C M E L L T K Q G W S S A Y S I E S V I M Q I N A T L V K G K A R V Q F G A N K N Q Y N L A R A Q Q S Y N S I V Q I H E K N G W Y T P P K E D G
- Accession
- Q8WVN8
- Protein length
- Full Length
- Predicted molecular weight
- 45.2 kDa
- Amino acids
- 1 to 375
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab201879 was purified using conventional chromatography techniques.
General info
- Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination.
- Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
- Post-translational modifications
Auto-ubiquitinated in vitro.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UBE2Q2 also known as Ubiquitin-Conjugating Enzyme E2 Q2 is a member of the E2 ubiquitin-conjugating enzyme family with a molecular mass of approximately 48 kDa. It plays a mechanical role in the ubiquitination process by transferring activated ubiquitin from E1-carrying enzymes to target substrates tagging them for proteasomal degradation. This protein is widely expressed in various tissues including those in the brain liver and lungs.
- Biological function summary
UBE2Q2 contributes to protein homeostasis by participating in the ubiquitin-proteasome pathway which maintains protein turnover within cells. It associates with E3 ligase complexes to select substrate specificity making it essential for regulating proteins related to cell cycle and growth. Research indicates its involvement in managing regulatory proteins that control cell division and apoptosis indicating its influence on cellular maintenance and stability.
- Pathways
Cellular growth and apoptosis processes involve UBE2Q2 in ubiquitin-mediated protein degradation pathways. It often collaborates with other ubiquitin-conjugating enzymes and E3 ligases such as CBL and NEDD4 focusing on protein quality control. The Wnt signaling pathway which is important for cell proliferation and differentiation also receives input from UBE2Q2 through its regulatory actions on beta-catenin turnover.
- Associated diseases and disorders
UBE2Q2 has connections to cancer development particularly colorectal and breast cancers. Aberrant expression or dysfunction of UBE2Q2 can lead to disrupted proteostasis and altered cellular signaling contributing to tumorigenesis. The oncogene CBL which interacts with UBE2Q2 is also implicated in these cancer types further highlighting UBE2Q2's role in cancer pathology.
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1 product image
SDS-PAGE - Recombinant Human UBE2Q2 protein (ab201879)
15% SDS-PAGE analysis of ab201879 (3μg).
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