Recombinant Human UBE2S protein (His tag N-Terminus)
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Recombinant Human UBE2S protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.
View Alternative Names
E2EPF, OK/SW-cl.73, UBE2S, Ubiquitin-conjugating enzyme E2 S, E2 ubiquitin-conjugating enzyme S, E2-EPF, Ubiquitin carrier protein S, Ubiquitin-conjugating enzyme E2-24 kDa, Ubiquitin-conjugating enzyme E2-EPF5, Ubiquitin-protein ligase S
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human UBE2S protein (His tag N-Terminus) (AB87756)
15% SDS-PAGE analysis of 3μg ab87756.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
UBE2S acts within the anaphase-promoting complex/cyclosome (APC/C) an important multi-subunit E3 ubiquitin ligase. Through its activity UBE2S facilitates the progression of the mitotic cycle by tagging key regulatory proteins for degradation aiding in their timely removal during cell division. Without this regulation cells struggle with correct chromosomal segregation which can lead to genomic instability.
Pathways
UBE2S functions notably within the cell cycle regulation and protein degradation pathways. It collaborates closely with the anaphase-promoting complex/cyclosome (APC/C) and cooperates with related proteins such as CDC20 and CDH1. By enabling the degradation of securin and cyclins UBE2S helps manage the transition from metaphase to anaphase ensuring cells divide properly in mitotic processes.
Specifications
Form
Liquid
Additional notes
ab87756 was purified using conventional chromatography techniques.
General info
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed : 19820702, PubMed : 19822757, PubMed : 22496338, PubMed : 27259151). Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis (PubMed : 19820702, PubMed : 19822757, PubMed : 27259151, PubMed : 27910872). Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit (PubMed : 19820702, PubMed : 19822757, PubMed : 27259151). Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A (PubMed : 16819549). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination (PubMed : 20061386, PubMed : 20622874).
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Post-translational modifications
Autoubiquitinated by the APC/C complex during G1, leading to its degradation by the proteasome.
Target data
Complementary Products
Product promise
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