JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB174061

Recombinant Human UBE2U protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human UBE2U protein is a Human Full Length protein, in the 1 to 226 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for WB.

View Alternative Names

Ubiquitin-conjugating enzyme E2 U, E2 ubiquitin-conjugating enzyme U, Ubiquitin carrier protein U, Ubiquitin-protein ligase U, UBE2U

1 Images
SDS-PAGE - Recombinant Human UBE2U protein (AB174061)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human UBE2U protein (AB174061)

SDS-PAGE showing ab174061

Key facts

Purity

>80% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

WB

applications

Biologically active

No

Accession

Q5VVX9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

style
left-column

Complementary Products

Proteins & Peptides

AB158734

Recombinant Human Interferon alpha10 protein

SDS-PAGE - Recombinant Human Interferon alpha10 protein (AB158734)

  • 0
  • 0
Primary Antibodies

AB175058

Anti-TNNT3 antibody - C-terminal

Western blot - Anti-TNNT3 antibody - C-terminal (AB175058)

  • 4
  • 1
Primary Antibodies

AB129880

Anti-Thymidine Kinase HHV1 antibody

Western blot - Anti-Thymidine Kinase HHV1 antibody (AB129880)

  • 0
  • 0
Primary Antibodies

AB303619

Anti-RNF168 antibody [EPR24239-2] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-RNF168 antibody [EPR24239-2] - BSA and Azide free (Detector) (AB303619)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MHGRAYLLLHRDFCDLKENNYKGITAKPVSEDMMEWEVEIEGLQNSVWQGLVFQLTIHFTSEYNYAPPVVKFITIPFHPNVDPHTGQPCIDFLDNPEKWNTNYTLSSILLALQVMLSNPVLENPVNLEAARILVKDESLYRTILRLFNRPLQMKDDSQELPKDPRKCIRPIKTTSFSDYYQTWSRIATSKATEYYRTPLLKVPNFIGQYYKWKKMDLQHQKEWNLK","proteinLength":"Full Length","predictedMolecularWeight":"52 kDa","actualMolecularWeight":null,"aminoAcidEnd":226,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q5VVX9","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBE2U also known as Ubiquitin-Conjugating Enzyme E2 U is an enzyme involved in the ubiquitination process which tags proteins for degradation. It has a molecular weight of approximately 44 kDa. This enzyme is expressed in a variety of human tissues but its expression levels can vary depending on the cell type and condition. UBE2U works within the central mechanism of protein homeostasis where it assists in attaching ubiquitin to substrate proteins.
Biological function summary

The process of ubiquitination that UBE2U contributes to is integral for protein turnover and regulation. UBE2U acts as a part of the ubiquitin-proteasome system (UPS) a complex that ensures proteins are adequately degraded and recycled. This process maintains cellular health by regulating proteins that control cycle progression DNA repair and signal transduction. Its role in the UPS signifies that it helps sustain several vital cellular processes and adaptability to environmental changes.

Pathways

UBE2U is intricately involved in the cellular proteostasis network. Particularly it participates in the protein ubiquitination pathway and the cell cycle control pathway. In these pathways UBE2U collaborates with other ubiquitin-conjugating enzymes and ubiquitin ligases to maintain precisely the balance of cell proliferation and apoptosis. Proteins such as E1 and E3 enzymes work alongside UBE2U to fine-tune this ubiquitination cascade.

UBE2U shows associations with certain cancer types due to its regulatory functions on cell proliferation. Abnormalities in its function can lead to disrupted cell cycle regulation and potentially foster oncogenesis. Additionally the misregulation of UBE2U has links to neurodegenerative diseases because of its role in protein quality control. Interactions with proteins like Parkin highlight its relevance in disorders where protein accumulation is a hallmark such as Parkinson's disease.
style
left-column

Specifications

Form

Liquid

Additional notes

Affinity purified.

style
left-column

General info

Function

Catalyzes the covalent attachment of ubiquitin to other proteins.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Post-translational modifications

Autoubiquitinated in vitro in the presence of UBR5.

style
left-column

Product protocols

style
left-column

Target data

Catalyzes the covalent attachment of ubiquitin to other proteins.
See full target information UBE2U
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com