Recombinant human UBE2V1 protein is a Human Full Length protein, in the 2 to 170 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
P G E V Q A S Y L K S Q S K L S D E G R L E P R K F H C K G V K V P R N F R L L E E L E E G Q K G V G D G T V S W G L E D D E D M T L T R W T G M I I G P P R T I Y E N R I Y S L K I E C G P K Y P E A P P F V R F V T K I N M N G V N S S N G V V D P R A I S V L A K W Q N S Y S I K V V L Q E L R R L M M S K E N M K L P Q P P E G Q C Y S N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UBE2N, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production (PubMed:31006531). UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway.
Alternative names
CROC1, UBE2V, UEV1, P/OKcl.19, UBE2V1, Ubiquitin-conjugating enzyme E2 variant 1, UEV-1, CROC-1, TRAF6-regulated IKK activator 1 beta Uev1A
Recommended products
Recombinant human UBE2V1 protein is a Human Full Length protein, in the 2 to 170 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- 50 μl reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, UBE1 (0.5 μg), UEV1A or other E2, Biotin-Ubiquitin (0.5 μg) and Smurf2 (2 μg) in 50 mM Tris, pH 7.4) for 30 minutes at 37°C. Add to Streptavidin-coated plates for 30 minutes at 37°C. Incubate with anti-Smurf2, then HRP-labeled secondary antibody and chemiluminescent detection
- Accession
- Q13404-4
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.72% Sodium chloride, 0.71% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- P G E V Q A S Y L K S Q S K L S D E G R L E P R K F H C K G V K V P R N F R L L E E L E E G Q K G V G D G T V S W G L E D D E D M T L T R W T G M I I G P P R T I Y E N R I Y S L K I E C G P K Y P E A P P F V R F V T K I N M N G V N S S N G V V D P R A I S V L A K W Q N S Y S I K V V L Q E L R R L M M S K E N M K L P Q P P E G Q C Y S N
- Accession
- Q13404
- Protein length
- Full Length
- Predicted molecular weight
- 20 kDa
- Amino acids
- 2 to 170
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Lot specific.
General info
- Function
Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UBE2N, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production (PubMed:31006531). UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway.
- Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UBE2V1 also known as ubiquitin-conjugating enzyme E2 variant 1 acts as an E2-like enzyme facilitating the transfer of ubiquitin molecules to substrate proteins. It is a non-canonical ubiquitin-conjugating enzyme because it lacks the active cysteine residue needed to conjugate ubiquitin directly. The molecular mass of UBE2V1 is approximately 20 kDa. UBE2V1 is highly expressed in various human tissues including the spleen liver and placenta suggesting its broad role in cellular functions.
- Biological function summary
UBE2V1 collaborates with UBE2N (also known as Ubc13) to form a heterodimer c. This dimer is involved in the process of K63-linked polyubiquitination which does not target proteins for proteasomal degradation but rather plays an important role in DNA damage tolerance and immune response regulation. UBE2V1 when associated with UBE2N aids in the polyubiquitination of protein substrates allowing for their involvement in regulatory mechanisms that are essential for cellular homeostasis.
- Pathways
UBE2V1 serves an important function in the NF-κB signaling pathway and DNA repair processes. In the NF-κB pathway UBE2V1 through its interaction with UBE2N contributes to the activation of TAK1 kinase which ultimately activates the transcription factor NF-κB leading to inflammatory and immune responses. In DNA repair it participates in error-free damage bypass mechanism alongside UBE2N. These pathways highlight the integration of UBE2V1 in cellular processes that manage stress and damage responses.
- Associated diseases and disorders
UBE2V1 associates with cancer and immune disorders. Its role in the NF-κB pathway links it to oncogenic processes as deregulated NF-κB signaling often corresponds with tumorigenesis. In addition abnormalities in UBE2V1’s function may lead to aberrant immune responses as its partnership with UBE2N influences inflammatory reactions. Such dysregulation can contribute to both the initiation and progression of diseases like rheumatoid arthritis and various cancers marking UBE2V1 as a potential target for therapeutic intervention.
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2 product images
SDS-PAGE - Recombinant human UBE2V1 protein (ab198472)
4-20% SDS PAGE analysis of 1 μg ab198472 stained with Coomassie
Functional Studies - Recombinant human UBE2V1 protein (ab198472)
Functional studies of ab198472
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