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AB289745

Recombinant Human UBF1 protein

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Recombinant Human UBF1 protein is a Human Full Length protein, in the 1 to 764 aa range, expressed in Cell free, with >90%, suitable for SDS-PAGE.

View Alternative Names

UBF, UBF1, UBTF, Nucleolar transcription factor 1, Autoantigen NOR-90, Upstream-binding factor 1, UBF-1

1 Images
SDS-PAGE - Recombinant Human UBF1 protein (AB289745)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human UBF1 protein (AB289745)

SDS-PAGE analysis of ab289745

Key facts

Purity

>90% SDS-PAGE

Expression system

Cell free

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P17480

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 - 8 Constituents: 6% Trehalose, 0.12% Tris, 0.037% Ethylenediaminetetraacetic acid, disodium, dihydrate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MNGEADCPTDLEMAAPKGQDRWSQEDMLTLLECMKNNLPSNDSSKFKTTESHMDWEKVAFKDFSGDMCKLKWVEISNEVRKFRTLTELILDAQEHVKNPYKGKKLKKHPDFPKKPTPYFRFFMEKRAKYAKLHPEMSNLDLTKILSKKYKELPEKKKMKYIQDFQREKQEFERNLARFREDHPLIQNAKKSDIPEKPKTPQQLWYTHEKKVYLKVRPDATTKEVKDSLGKQWSLSDKKRLKWIHKALEQRKEYEEIMRDYIQKHPELNISEEGITKSTLTKAERQLKDKFDGRPTKPPPNSYSLYCAELMANMKDVPSTERMVLCSQQWKLLSQKEKDAYHKKCDQKKKDYEELLRFLESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPVSAMFIFSEEKRRQLQEERPELSESELTRLLARMWNDLSEKKKAKYKAREAALKAQSERKPGGERERGKLPESPKRAEEIWQQSVIGDYLARFKNDRVKALKAMEMTWNNMEKKEKLMWIKKAAEDQKRYERELSEMRAPPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKEMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYISNKRKSMTKLRGPNPKSSRTTLQSKSESEEDDEEDEDDEDEDEEEEDDENGDSSEDGGDSSESSSEESEDGDENEEDDEDEDDDEDDDEDEDNESEGSSSSSSSSGDSSDSDSN","proteinLength":"Full Length","predictedMolecularWeight":"107.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":764,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Cell free","accessionNumber":"P17480","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBF1 also known as Upstream Binding Factor 1 is a transcription factor with a mass of approximately 97 kDa. It plays a mechanical role in facilitating the transcription of ribosomal RNA (rRNA) by binding to the core promoter element of rRNA genes. This action stimulates the recruitment of RNA polymerase I to the rDNA promoter site. UBF1 expresses in the nucleolus of eukaryotic cells where it functions as part of a nucleolar complex. Its presence is important for the structural organization and function of the nucleolus.
Biological function summary

UBF1 is essential for the regulation of ribosomal biogenesis. It binds to the rDNA enhancer and the rDNA promoter as part of the transcription initiation complex helping to modulate the synthesis of rRNA. UBF1 works synergistically with other nucleolar proteins such as SL1 and TIF-IA. This complex behavior influences cellular growth and proliferation. The interaction of UBF1 acting either alone or in combination with factors like SL1 controls the transcription rate of rRNA impacting the overall protein synthesis capacity of the cell.

Pathways

UBF1 plays a significant role in the cellular growth and ribosome biogenesis pathways due to its fundamental participation in rRNA transcription. It interacts closely with components of the RNA polymerase I transcription machinery. UBF1 also links to the mTOR signaling pathway which is pivotal for cell growth regulation. Interactions with other proteins such as RRN3 help mediate the link between transcriptional regulation by UBF1 and these broader signaling pathways.

UBF1 links to various pathological conditions especially cancer and growth disorders. Aberrations in UBF1 expression or function can lead to unchecked cellular proliferation and tumorigenesis as seen in certain types of cancer. Its connection to the rRNA synthesis pathway implicates it in growth disorders associated with defective ribosome production. Altered interactions between UBF1 and other proteins like p53 play a role in these disease states either by disrupting normal cell cycle control or by enabling the continued presence of harmful mutations.
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Specifications

Form

Lyophilized

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General info

Function

Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I (Pol I) through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element and can activate Pol I promoter escape.

Post-translational modifications

Phosphorylated and activated by PIK3CA.

Subcellular localisation

Nucleus

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I (Pol I) through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element and can activate Pol I promoter escape.
See full target information UBTF
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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