Recombinant Human Ubiquilin/UBQLN1 protein is a Human Full Length protein, in the 1 to 589 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M A E S G E S G G P P G S Q D S A A G A E G A G T P A A A A S A E P K I M K V T V K T P K E K E E F A V P E N S S V Q Q F K E E I S K R F K S H T D Q L V L I F A G K I L K D Q D T L S Q H G I H D G L T V H L V I K T Q N R P Q D H S A Q Q T N T A G S N V T T S S T P N S N S T S G S A T S N P F G L G G L G G L A G L S S L G L N T T N F S E L Q S Q M Q R Q L L S N P E M M V Q I M E N P F V Q S M L S N H D L M R Q L I M A N P Q M Q Q L I Q R N P E I S H M L N N P D I M R Q T L E L A R N P A M M Q E M M R N Q D R A L S N L E S I P G G Y N A L R R M Y T D I Q E P M L S A A Q E Q F G G N P F A S L V S N T S S G E G S Q P S R T E N R D P L P N P W A P Q T S Q S S S A S S G T A S T V G G T T G S T A S G T S G Q S T T A P N L V P G V G A S M F N T P G M Q S L L Q Q I T E N P Q L M Q N M L S A P Y M R S M M Q S L S Q N P D L A A Q M M L N N P L F A G N P Q L Q E Q M R Q Q L P T F L Q Q M Q N P D T L S A M S N P R A M Q A L L Q I Q Q G L Q T L A T E A P G L I P G F T P G L G A L G S T G G S S G T N G S N A T P S E N T S P T A G T T E P G H Q Q F I Q Q M L Q A L A G V N P Q L Q N P E V R F Q Q Q L E Q L S A M G F L N R E A N L Q A L I A T G G D I N A A I E R L L G S Q P S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and endoplasmic reticulum-associated protein degradation (ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome (PubMed:15147878). Plays a role in the ERAD pathway via its interaction with ER-localized proteins UBXN4, VCP and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome (PubMed:18307982, PubMed:19822669). Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion (PubMed:19148225, PubMed:20529957, PubMed:23459205). Negatively regulates the TICAM1/TRIF-dependent toll-like receptor signaling pathway by decreasing the abundance of TICAM1 via the autophagic pathway (PubMed:21695056). Promotes the ubiquitination and lysosomal degradation of ORAI1, consequently down-regulating the ORAI1-mediated Ca2+ mobilization (PubMed:23307288). Suppresses the maturation and proteasomal degradation of amyloid beta A4 protein (A4) by stimulating the lysine 63 (K63)-linked polyubiquitination. Delays the maturation of A4 by sequestering it in the Golgi apparatus and preventing its transport to the cell surface for subsequent processing (By similarity). Ubiquitinates BCL2L10 and thereby stabilizes protein abundance (PubMed:22233804). Isoform 1. Plays a role in unfolded protein response (UPR) by attenuating the induction of UPR-inducible genes, DDTI3/CHOP, HSPA5 and PDIA2 during ER stress (PubMed:18953672). Plays a key role in the regulation of the levels of PSEN1 by targeting its accumulation to aggresomes which may then be removed from cells by autophagocytosis (PubMed:21143716). Isoform 2. Plays a role in unfolded protein response (UPR) by attenuating the induction of UPR-inducible genes, DDTI3/CHOP, HSPA5 and PDIA2 during ER stress. Isoform 3. Plays a role in unfolded protein response (UPR) by attenuating the induction of UPR-inducible genes, DDTI3/CHOP, HSPA5 and PDIA2 during ER stress (PubMed:18953672). Plays a key role in the regulation of the levels of PSEN1 by targeting its accumulation to aggresomes which may then be removed from cells by autophagocytosis (PubMed:21143716).
Alternative names
DA41, PLIC1, UBQLN1, Ubiquilin-1, Protein linking IAP with cytoskeleton 1, PLIC-1, hPLIC-1
Recommended products
Recombinant Human Ubiquilin/UBQLN1 protein is a Human Full Length protein, in the 1 to 589 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9UMX0-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A E S G E S G G P P G S Q D S A A G A E G A G T P A A A A S A E P K I M K V T V K T P K E K E E F A V P E N S S V Q Q F K E E I S K R F K S H T D Q L V L I F A G K I L K D Q D T L S Q H G I H D G L T V H L V I K T Q N R P Q D H S A Q Q T N T A G S N V T T S S T P N S N S T S G S A T S N P F G L G G L G G L A G L S S L G L N T T N F S E L Q S Q M Q R Q L L S N P E M M V Q I M E N P F V Q S M L S N H D L M R Q L I M A N P Q M Q Q L I Q R N P E I S H M L N N P D I M R Q T L E L A R N P A M M Q E M M R N Q D R A L S N L E S I P G G Y N A L R R M Y T D I Q E P M L S A A Q E Q F G G N P F A S L V S N T S S G E G S Q P S R T E N R D P L P N P W A P Q T S Q S S S A S S G T A S T V G G T T G S T A S G T S G Q S T T A P N L V P G V G A S M F N T P G M Q S L L Q Q I T E N P Q L M Q N M L S A P Y M R S M M Q S L S Q N P D L A A Q M M L N N P L F A G N P Q L Q E Q M R Q Q L P T F L Q Q M Q N P D T L S A M S N P R A M Q A L L Q I Q Q G L Q T L A T E A P G L I P G F T P G L G A L G S T G G S S G T N G S N A T P S E N T S P T A G T T E P G H Q Q F I Q Q M L Q A L A G V N P Q L Q N P E V R F Q Q Q L E Q L S A M G F L N R E A N L Q A L I A T G G D I N A A I E R L L G S Q P S
- Accession
- Q9UMX0
- Protein length
- Full Length
- Amino acids
- 1 to 589
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and endoplasmic reticulum-associated protein degradation (ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome (PubMed:15147878). Plays a role in the ERAD pathway via its interaction with ER-localized proteins UBXN4, VCP and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome (PubMed:18307982, PubMed:19822669). Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion (PubMed:19148225, PubMed:20529957, PubMed:23459205). Negatively regulates the TICAM1/TRIF-dependent toll-like receptor signaling pathway by decreasing the abundance of TICAM1 via the autophagic pathway (PubMed:21695056). Promotes the ubiquitination and lysosomal degradation of ORAI1, consequently down-regulating the ORAI1-mediated Ca2+ mobilization (PubMed:23307288). Suppresses the maturation and proteasomal degradation of amyloid beta A4 protein (A4) by stimulating the lysine 63 (K63)-linked polyubiquitination. Delays the maturation of A4 by sequestering it in the Golgi apparatus and preventing its transport to the cell surface for subsequent processing (By similarity). Ubiquitinates BCL2L10 and thereby stabilizes protein abundance (PubMed:22233804).
- Post-translational modifications
Degraded during both macroautophagy and during chaperone-mediated autophagy (CMA).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Ubiquilin.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ubiquilin also known as UBQLN1 is a protein that plays a significant role in protein quality control processes. It has a molecular mass of approximately 62 kDa. Ubiquilin is widely expressed in various human tissues notably in the brain heart and skeletal muscle. It facilitates the delivery of misfolded proteins to the proteasome for degradation assisting in maintaining protein homeostasis. This function is essential in preventing the accumulation of dysfunctional proteins which could otherwise be toxic to cells.
- Biological function summary
Ubiquilin/UBQLN1 serves as a shuttle factor a component in the intricate network regulating protein degradation. It associates with ubiquitinated proteins and guides them to proteasomes or autophagosomes for disposal. Moreover Ubiquilin forms a complex with heat shock proteins and proteasome components enhancing its role in managing cellular protein quality control. Its activity actively influences proteostasis ensuring the careful balance of protein synthesis and breakdown.
- Pathways
Ubiquilin/UBQLN1 is integral to the ubiquitin-proteasome system and autophagy pathways. In these pathways Ubiquilin partners with proteins such as ubiquitin ligases and various adaptor proteins to mediate the clearance of misfolded or excess proteins therefore preventing cellular damage. Its interaction with the chaperone HSP70 is critical for delivering proteins tagged for degradation. Through these pathways Ubiquilin influences cellular stress responses and protein turnover maintaining cellular function and health.
- Associated diseases and disorders
Abnormalities in Ubiquilin/UBQLN1 have been associated with neurodegenerative diseases and cancer. Altered UBQLN1 function or expression contributes to Alzheimer's disease linked to protein aggregation and neuronal damage. Additionally mutations or dysregulation of UBQLN1 are observed in certain cancers where disrupted protein homeostasis promotes tumorigenesis. Here it's involvement with proteins like presenilin-1 and the amyloid precursor protein emphasizes its contributions to disease states making it a focus for therapeutic research.
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1 product image
SDS-PAGE - Recombinant Human Ubiquilin/UBQLN1 protein (ab153310)
ab153310 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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