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AB153310

Recombinant Human Ubiquilin/UBQLN1 protein (GST tag N-Terminus)

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Recombinant Human Ubiquilin/UBQLN1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 589 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

DA41, PLIC1, UBQLN1, Ubiquilin-1, Protein linking IAP with cytoskeleton 1, PLIC-1, hPLIC-1

1 Images
SDS-PAGE - Recombinant Human Ubiquilin/UBQLN1 protein (GST tag N-Terminus) (AB153310)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Ubiquilin/UBQLN1 protein (GST tag N-Terminus) (AB153310)

ab153310 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q9UMX0

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB3341

Anti-Ubiquilin/UBQLN1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (AB3341)

  • 4
  • 1
Proteins & Peptides

AB207109

Recombinant Human UBQLN2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human UBQLN2 protein (His tag N-Terminus) (AB207109)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB170901

Anti-SYVN1/HRD1 antibody [EP7459]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SYVN1/HRD1 antibody [EP7459] (AB170901)

  • 3
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as Ubiquilin.
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Sequence info

[{"sequence":"MAESGESGGPPGSQDSAAGAEGAGTPAAAASAEPKIMKVTVKTPKEKEEFAVPENSSVQQFKEEISKRFKSHTDQLVLIFAGKILKDQDTLSQHGIHDGLTVHLVIKTQNRPQDHSAQQTNTAGSNVTTSSTPNSNSTSGSATSNPFGLGGLGGLAGLSSLGLNTTNFSELQSQMQRQLLSNPEMMVQIMENPFVQSMLSNHDLMRQLIMANPQMQQLIQRNPEISHMLNNPDIMRQTLELARNPAMMQEMMRNQDRALSNLESIPGGYNALRRMYTDIQEPMLSAAQEQFGGNPFASLVSNTSSGEGSQPSRTENRDPLPNPWAPQTSQSSSASSGTASTVGGTTGSTASGTSGQSTTAPNLVPGVGASMFNTPGMQSLLQQITENPQLMQNMLSAPYMRSMMQSLSQNPDLAAQMMLNNPLFAGNPQLQEQMRQQLPTFLQQMQNPDTLSAMSNPRAMQALLQIQQGLQTLATEAPGLIPGFTPGLGALGSTGGSSGTNGSNATPSENTSPTAGTTEPGHQQFIQQMLQALAGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":589,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9UMX0","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ubiquilin also known as UBQLN1 is a protein that plays a significant role in protein quality control processes. It has a molecular mass of approximately 62 kDa. Ubiquilin is widely expressed in various human tissues notably in the brain heart and skeletal muscle. It facilitates the delivery of misfolded proteins to the proteasome for degradation assisting in maintaining protein homeostasis. This function is essential in preventing the accumulation of dysfunctional proteins which could otherwise be toxic to cells.
Biological function summary

Ubiquilin/UBQLN1 serves as a shuttle factor a component in the intricate network regulating protein degradation. It associates with ubiquitinated proteins and guides them to proteasomes or autophagosomes for disposal. Moreover Ubiquilin forms a complex with heat shock proteins and proteasome components enhancing its role in managing cellular protein quality control. Its activity actively influences proteostasis ensuring the careful balance of protein synthesis and breakdown.

Pathways

Ubiquilin/UBQLN1 is integral to the ubiquitin-proteasome system and autophagy pathways. In these pathways Ubiquilin partners with proteins such as ubiquitin ligases and various adaptor proteins to mediate the clearance of misfolded or excess proteins therefore preventing cellular damage. Its interaction with the chaperone HSP70 is critical for delivering proteins tagged for degradation. Through these pathways Ubiquilin influences cellular stress responses and protein turnover maintaining cellular function and health.

Abnormalities in Ubiquilin/UBQLN1 have been associated with neurodegenerative diseases and cancer. Altered UBQLN1 function or expression contributes to Alzheimer's disease linked to protein aggregation and neuronal damage. Additionally mutations or dysregulation of UBQLN1 are observed in certain cancers where disrupted protein homeostasis promotes tumorigenesis. Here it's involvement with proteins like presenilin-1 and the amyloid precursor protein emphasizes its contributions to disease states making it a focus for therapeutic research.
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Specifications

Form

Liquid

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General info

Function

Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and endoplasmic reticulum-associated protein degradation (ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome (PubMed : 15147878). Plays a role in the ERAD pathway via its interaction with ER-localized proteins UBXN4, VCP and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome (PubMed : 18307982, PubMed : 19822669). Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion (PubMed : 19148225, PubMed : 20529957, PubMed : 23459205). Negatively regulates the TICAM1/TRIF-dependent toll-like receptor signaling pathway by decreasing the abundance of TICAM1 via the autophagic pathway (PubMed : 21695056). Promotes the ubiquitination and lysosomal degradation of ORAI1, consequently down-regulating the ORAI1-mediated Ca2+ mobilization (PubMed : 23307288). Suppresses the maturation and proteasomal degradation of amyloid beta A4 protein (A4) by stimulating the lysine 63 (K63)-linked polyubiquitination. Delays the maturation of A4 by sequestering it in the Golgi apparatus and preventing its transport to the cell surface for subsequent processing (By similarity). Ubiquitinates BCL2L10 and thereby stabilizes protein abundance (PubMed : 22233804).. Isoform 1. Plays a role in unfolded protein response (UPR) by attenuating the induction of UPR-inducible genes, DDTI3/CHOP, HSPA5 and PDIA2 during ER stress (PubMed : 18953672). Plays a key role in the regulation of the levels of PSEN1 by targeting its accumulation to aggresomes which may then be removed from cells by autophagocytosis (PubMed : 21143716).. Isoform 2. Plays a role in unfolded protein response (UPR) by attenuating the induction of UPR-inducible genes, DDTI3/CHOP, HSPA5 and PDIA2 during ER stress.. Isoform 3. Plays a role in unfolded protein response (UPR) by attenuating the induction of UPR-inducible genes, DDTI3/CHOP, HSPA5 and PDIA2 during ER stress (PubMed : 18953672). Plays a key role in the regulation of the levels of PSEN1 by targeting its accumulation to aggresomes which may then be removed from cells by autophagocytosis (PubMed : 21143716).

Post-translational modifications

Degraded during both macroautophagy and during chaperone-mediated autophagy (CMA).. Phosphorylated.. Ubiquitinated.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and endoplasmic reticulum-associated protein degradation (ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome (PubMed : 15147878). Plays a role in the ERAD pathway via its interaction with ER-localized proteins UBXN4, VCP and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome (PubMed : 18307982, PubMed : 19822669). Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion (PubMed : 19148225, PubMed : 20529957, PubMed : 23459205). Negatively regulates the TICAM1/TRIF-dependent toll-like receptor signaling pathway by decreasing the abundance of TICAM1 via the autophagic pathway (PubMed : 21695056). Promotes the ubiquitination and lysosomal degradation of ORAI1, consequently down-regulating the ORAI1-mediated Ca2+ mobilization (PubMed : 23307288). Suppresses the maturation and proteasomal degradation of amyloid beta A4 protein (A4) by stimulating the lysine 63 (K63)-linked polyubiquitination. Delays the maturation of A4 by sequestering it in the Golgi apparatus and preventing its transport to the cell surface for subsequent processing (By similarity). Ubiquitinates BCL2L10 and thereby stabilizes protein abundance (PubMed : 22233804).. Isoform 1. Plays a role in unfolded protein response (UPR) by attenuating the induction of UPR-inducible genes, DDTI3/CHOP, HSPA5 and PDIA2 during ER stress (PubMed : 18953672). Plays a key role in the regulation of the levels of PSEN1 by targeting its accumulation to aggresomes which may then be removed from cells by autophagocytosis (PubMed : 21143716).. Isoform 2. Plays a role in unfolded protein response (UPR) by attenuating the induction of UPR-inducible genes, DDTI3/CHOP, HSPA5 and PDIA2 during ER stress.. Isoform 3. Plays a role in unfolded protein response (UPR) by attenuating the induction of UPR-inducible genes, DDTI3/CHOP, HSPA5 and PDIA2 during ER stress (PubMed : 18953672). Plays a key role in the regulation of the levels of PSEN1 by targeting its accumulation to aggresomes which may then be removed from cells by autophagocytosis (PubMed : 21143716).
See full target information UBQLN1
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