Recombinant Human Ubiquinol-Cytochrome C Reductase Core Protein I (denatured) is a Human Full Length protein, in the 35 to 480 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S E F T A T F A Q A L Q F V P E T Q V S L L D N G L R V A S E Q S S Q P T C T V G V W I D V G S R F E T E K N N G A G Y F L E H L A F K G T K N R P G S A L E K E V E S M G A H L N A Y S T R E H T A Y Y I K A L S K D L P K A V E L L G D I V Q N C S L E D S Q I E K E R D V I L R E M Q E N D A S M R D V V F N Y L H A T A F Q G T P L A Q A V E G P S E N V R K L S R A D L T E Y L S T H Y K A P R M V L A A A G G V E H Q Q L L D L A Q K H L G G I P W T Y A E D A V P T L T P C R F T G S E I R H R D D A L P F A H V A I A V E G P G W A S P D N V A L Q V A N A I I G H Y D C T Y G G G V H L S S P L A S G A V A N K L C Q S F Q T F S I C Y A E T G L L G A H F V C D R M K I D D M M F V L Q G Q W M R L C T S A T E S E V A R G K N I L R N A L V S H L D G T T P V C E D I G R S L L T Y G R R I P L A E W E S R I A E V D A S V V R E I C S K Y I Y D Q C P A V A G Y G P I E Q L P D Y N R I R S G M F W L R F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c (By similarity). The 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 are homologous to the 2 mitochondrial-processing peptidase (MPP) subunits beta-MPP and alpha-MPP respectively, and they seem to have preserved their MPP processing properties (By similarity). May be involved in the in situ processing of UQCRFS1 into the mature Rieske protein and its mitochondrial targeting sequence (MTS)/subunit 9 when incorporated into complex III (Probable).
Alternative names
Complex III subunit 1, Core protein I, Ubiquinol-cytochrome-c reductase complex core protein 1, UQCRC1
Recommended products
Recombinant Human Ubiquinol-Cytochrome C Reductase Core Protein I (denatured) is a Human Full Length protein, in the 35 to 480 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P31930-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris-HCl buffer
Sequence info
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S E F T A T F A Q A L Q F V P E T Q V S L L D N G L R V A S E Q S S Q P T C T V G V W I D V G S R F E T E K N N G A G Y F L E H L A F K G T K N R P G S A L E K E V E S M G A H L N A Y S T R E H T A Y Y I K A L S K D L P K A V E L L G D I V Q N C S L E D S Q I E K E R D V I L R E M Q E N D A S M R D V V F N Y L H A T A F Q G T P L A Q A V E G P S E N V R K L S R A D L T E Y L S T H Y K A P R M V L A A A G G V E H Q Q L L D L A Q K H L G G I P W T Y A E D A V P T L T P C R F T G S E I R H R D D A L P F A H V A I A V E G P G W A S P D N V A L Q V A N A I I G H Y D C T Y G G G V H L S S P L A S G A V A N K L C Q S F Q T F S I C Y A E T G L L G A H F V C D R M K I D D M M F V L Q G Q W M R L C T S A T E S E V A R G K N I L R N A L V S H L D G T T P V C E D I G R S L L T Y G R R I P L A E W E S R I A E V D A S V V R E I C S K Y I Y D Q C P A V A G Y G P I E Q L P D Y N R I R S G M F W L R F
- Accession
- Protein length
Full Length
- Predicted molecular weight
51.8 kDa
- Amino acids
35 to 480
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
ab177632 was purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
General info
- Function
Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c (By similarity). The 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 are homologous to the 2 mitochondrial-processing peptidase (MPP) subunits beta-MPP and alpha-MPP respectively, and they seem to have preserved their MPP processing properties (By similarity). May be involved in the in situ processing of UQCRFS1 into the mature Rieske protein and its mitochondrial targeting sequence (MTS)/subunit 9 when incorporated into complex III (Probable).
- Sequence similarities
Belongs to the peptidase M16 family. UQCRC1/QCR1 subfamily.
- Subcellular localisation
Mitochondrion inner membrane
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ubiquinol-Cytochrome C Reductase Core Protein I also known as Core Protein I of the cytochrome c reductase complex functions as part of the mitochondrial electron transport chain. It has a molecular weight of around 52 kDa. The protein plays an integral role within the inner mitochondrial membrane primarily performing mechanical roles in electron transfer. Ubiquinol-Cytochrome C Reductase Core Protein I also goes by its abbreviated symbol UQCRC1 and is expressed broadly across tissues with high energy demands.
- Biological function summary
UQCRC1 acts as a core component of Complex III also referred to as Cytochrome bc1 complex. This complex sits centrally within the mitochondrial respiratory chain catalyzing the transfer of electrons from ubiquinol to cytochrome c. By facilitating this electron transfer UQCRC1 aids in generating a proton gradient across the inner mitochondrial membrane essential for ATP synthesis during oxidative phosphorylation.
- Pathways
UQCRC1 finds itself prominently embedded in the oxidative phosphorylation and electron transport chain pathways. It directly interacts with other proteins such as cytochrome c and cytochrome reductase emphasizing its cooperative function in maintaining cellular energy balance. The seamless integration of UQCRC1 into these pathways highlights its importance in sustaining efficient mitochondrial operation a critical element for cellular respiration.
- Associated diseases and disorders
UQCRC1 shows connections to conditions involving mitochondrial dysfunction such as Leigh syndrome and mitochondrial myopathy. These disorders sometimes exhibit impaired electron transport impacting ATP production. Proteins like cytochrome c and other cytochrome reductase components often participate in these disorder pathways indicating potential targets for therapeutic interventions that aim to restore or augment mitochondrial function.
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SDS-PAGE - Recombinant Human Ubiquinol-Cytochrome C Reductase Core Protein I (denatured) (ab177632)
15% SDS-PAGE analysis of ab177632 (3 μg).
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