Recombinant Human UBQLN2 protein is a Human Full Length protein, in the 1 to 624 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A E N G E S S G P P R P S R G P A A A Q G S A A A P A E P K I I K V T V K T P K E K E E F A V P E N S S V Q Q F K E A I S K R F K S Q T D Q L V L I F A G K I L K D Q D T L I Q H G I H D G L T V H L V I K S Q N R P Q G Q S T Q P S N A A G T N T T S A S T P R S N S T P I S T N S N P F G L G S L G G L A G L S S L G L S S T N F S E L Q S Q M Q Q Q L M A S P E M M I Q I M E N P F V Q S M L S N P D L M R Q L I M A N P Q M Q Q L I Q R N P E I S H L L N N P D I M R Q T L E I A R N P A M M Q E M M R N Q D L A L S N L E S I P G G Y N A L R R M Y T D I Q E P M L N A A Q E Q F G G N P F A S V G S S S S S G E G T Q P S R T E N R D P L P N P W A P P P A T Q S S A T T S T T T S T G S G S G N S S S N A T G N T V A A A N Y V A S I F S T P G M Q S L L Q Q I T E N P Q L I Q N M L S A P Y M R S M M Q S L S Q N P D L A A Q M M L N S P L F T A N P Q L Q E Q M R P Q L P A F L Q Q M Q N P D T L S A M S N P R A M Q A L M Q I Q Q G L Q T L A T E A P G L I P S F T P G V G V G V L G T A I G P V G P V T P I G P I G P I V P F T P I G P I G P I G P T G P A A P P G S T G S G G P T G P T V S S A A P S E T T S P T S E S G P N Q Q F I Q Q M V Q A L A G A N A P Q L P N P E V R F Q Q Q L E Q L N A M G F L N R E A N L Q A L I A T G G D I N A A I E R L L G S Q P S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and the endoplasmic reticulum-associated protein degradation (ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome (PubMed:10983987). Plays a role in the ERAD pathway via its interaction with ER-localized proteins FAF2/UBXD8 and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome (PubMed:18307982, PubMed:24215460). Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion (PubMed:19148225, PubMed:20529957). Negatively regulates the endocytosis of GPCR receptors: AVPR2 and ADRB2, by specifically reducing the rate at which receptor-arrestin complexes concentrate in clathrin-coated pits (CCPs) (PubMed:18199683).
Alternative names
N4BP4, PLIC2, HRIHFB2157, UBQLN2, Ubiquilin-2, Chap1, DSK2 homolog, Protein linking IAP with cytoskeleton 2, Ubiquitin-like product Chap1/Dsk2, PLIC-2, hPLIC-2
Recommended products
Recombinant Human UBQLN2 protein is a Human Full Length protein, in the 1 to 624 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9UHD9-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A E N G E S S G P P R P S R G P A A A Q G S A A A P A E P K I I K V T V K T P K E K E E F A V P E N S S V Q Q F K E A I S K R F K S Q T D Q L V L I F A G K I L K D Q D T L I Q H G I H D G L T V H L V I K S Q N R P Q G Q S T Q P S N A A G T N T T S A S T P R S N S T P I S T N S N P F G L G S L G G L A G L S S L G L S S T N F S E L Q S Q M Q Q Q L M A S P E M M I Q I M E N P F V Q S M L S N P D L M R Q L I M A N P Q M Q Q L I Q R N P E I S H L L N N P D I M R Q T L E I A R N P A M M Q E M M R N Q D L A L S N L E S I P G G Y N A L R R M Y T D I Q E P M L N A A Q E Q F G G N P F A S V G S S S S S G E G T Q P S R T E N R D P L P N P W A P P P A T Q S S A T T S T T T S T G S G S G N S S S N A T G N T V A A A N Y V A S I F S T P G M Q S L L Q Q I T E N P Q L I Q N M L S A P Y M R S M M Q S L S Q N P D L A A Q M M L N S P L F T A N P Q L Q E Q M R P Q L P A F L Q Q M Q N P D T L S A M S N P R A M Q A L M Q I Q Q G L Q T L A T E A P G L I P S F T P G V G V G V L G T A I G P V G P V T P I G P I G P I V P F T P I G P I G P I G P T G P A A P P G S T G S G G P T G P T V S S A A P S E T T S P T S E S G P N Q Q F I Q Q M V Q A L A G A N A P Q L P N P E V R F Q Q Q L E Q L N A M G F L N R E A N L Q A L I A T G G D I N A A I E R L L G S Q P S
- Accession
- Q9UHD9
- Protein length
- Full Length
- Predicted molecular weight
- 68.1 kDa
- Amino acids
- 1 to 624
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purified by using conventional chromatography techniques.
General info
- Function
Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and the endoplasmic reticulum-associated protein degradation (ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome (PubMed:10983987). Plays a role in the ERAD pathway via its interaction with ER-localized proteins FAF2/UBXD8 and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome (PubMed:18307982, PubMed:24215460). Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion (PubMed:19148225, PubMed:20529957). Negatively regulates the endocytosis of GPCR receptors: AVPR2 and ADRB2, by specifically reducing the rate at which receptor-arrestin complexes concentrate in clathrin-coated pits (CCPs) (PubMed:18199683).
- Post-translational modifications
Degraded during macroautophagy.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UBQLN2 also known as ubiquitin-like protein UBQLN2 or ubiquilin-2 functions as part of the ubiquitin-proteasome system. It helps in shuttling ubiquitinated proteins to the proteasome for degradation. UBQLN2 has a molecular mass of approximately 66 kDa. Diverse tissues express UBQLN2 including brain kidney and skeletal muscle indicating its widespread importance in various cellular processes.
- Biological function summary
UBQLN2 interacts with other proteins involved in protein quality control. It participates in complexes that manage protein homeostasis by assisting in the degradation of misfolded or damaged proteins. This action prevents the accumulation of toxic protein aggregates. UBQLN2's role in the cellular protein degradation pathway highlights its importance in maintaining cellular function and integrity.
- Pathways
UBQLN2 engages in the endoplasmic reticulum-associated degradation (ERAD) pathway which ensures the elimination of misfolded proteins from the ER. It also links to the autophagy-lysosome pathway. UBQLN2 interacts with VCP/p97 in these pathways an important protein that assists in extracting ubiquitinated proteins for degradation. Through these interactions UBQLN2 contributes a pivotal role in maintaining cellular homeostasis.
- Associated diseases and disorders
Researchers have linked UBQLN2 mutations to amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Abnormalities in UBQLN2 may lead to the accumulation of protein aggregates harmful to neuronal cells. Connections between UBQLN2 and other proteins such as TARDBP (TDP-43) further emphasize its relevance as TDP-43 pathology is common in these neurodegenerative disorders. Understanding UBQLN2's function provides insight into potential therapeutic targets for these diseases.
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1 product image
SDS-PAGE - Recombinant Human UBQLN2 protein (ab207109)
15% SDS-PAGE analysis of ab207109 (3μg).
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