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AB167847

Recombinant Human UBXN2B protein (His tag N-Terminus)

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Recombinant Human UBXN2B protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 331 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

UBX domain-containing protein 2B, NSFL1 cofactor p37, p97 cofactor p37, UBXN2B

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SDS-PAGE - Recombinant Human UBXN2B protein (His tag N-Terminus) (AB167847)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human UBXN2B protein (His tag N-Terminus) (AB167847)

15% SDS-PAGE analysis of 3µg ab167847.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q14CS0

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAEGGGPEPGEQERRSSGPRPPSARDLQLALAELYEDEVKCKSSKSNRPKATVFKSPRTPPQRFYSSEHEYSGLNIVRPSTGKIVNELFKEAREHGAVPLNEATRASGDDKSKSFTGGGYRLGSSFCKRSEYIYGENQLQDVQILLKLWSNGFSLDDGELRPYNEPTNAQFLESVKRGEIPLELQRLVHGGQVNLDMEDHQDQEYIKPRLRFKAFSGEGQKLGSLTPEIVSTPSSPEEEDKSILNAVVLIDDSVPTTKIQIRLADGSRLIQRFNSTHRILDVRNFIVQSRPEFAALDFILVTSFPNKELTDESLTLLEADILNTVLLQQLK","proteinLength":"Full Length","predictedMolecularWeight":"39.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":331,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q14CS0","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBXN2B also known as UBX domain-containing protein 2B is a human protein with a molecular mass of approximately 60 kDa. It contains a UBX domain that enables interactions with various cellular components. UBXN2B is largely expressed in the cytoplasm and nucleus of cells. The protein acts as an adaptor in the ubiquitin-proteasome system facilitating the delivery of ubiquitinated substrates for degradation. It also interacts with p97/VCP an important AAA ATPase involved in extracting misfolded proteins.
Biological function summary

UBXN2B plays a significant role in the endoplasmic reticulum-associated degradation (ERAD) pathway managing protein quality control. It is an essential part of protein complexes that regulate ER-associated protein degradation. These complexes ensure proper folding and elimination of misfolded proteins which helps maintain cellular homeostasis. Lack of proper function of such complexes can lead to accumulation of defective proteins impacting cell health.

Pathways

UBXN2B participates in the protein degradation pathways including the ERAD and ubiquitin-proteasome pathways. It works closely with the p97/VCP complex to recognize and dismantle faulty proteins. UBXN2B also associates with other proteins in the degradation system such as ubiquitin ligases which tag unwanted proteins for destruction. This facilitates the cycling and reuse of cellular components essential for cellular metabolism and function.

UBXN2B has been linked to neurodegenerative diseases and cancer. Misregulation may contribute to conditions like Parkinson’s disease where protein aggregation negatively affects neurons. In certain cancers altered UBXN2B expression influences tumor growth by disrupting normal protein degradation pathways. These conditions often involve proteins like p97/VCP which under normal circumstances works with UBXN2B to maintain cellular proteostasis.
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Specifications

Form

Liquid

Additional notes

ab167847 was purified using conventional chromatography techniques.

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General info

Function

Adapter protein required for Golgi and endoplasmic reticulum biogenesis (PubMed : 17141156). Involved in Golgi and endoplasmic reticulum maintenance during interphase and in their reassembly at the end of mitosis (PubMed : 17141156). The complex formed with VCP has membrane fusion activity; membrane fusion activity requires USO1-GOLGA2 tethering and BET1L (PubMed : 17141156). VCPIP1 is also required, but not its deubiquitinating activity (PubMed : 17141156). Together with NSFL1C/p47, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase (PubMed : 23649807). Also, regulates spindle orientation during mitosis (PubMed : 23649807).

Sequence similarities

Belongs to the NSFL1C family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Adapter protein required for Golgi and endoplasmic reticulum biogenesis (PubMed : 17141156). Involved in Golgi and endoplasmic reticulum maintenance during interphase and in their reassembly at the end of mitosis (PubMed : 17141156). The complex formed with VCP has membrane fusion activity; membrane fusion activity requires USO1-GOLGA2 tethering and BET1L (PubMed : 17141156). VCPIP1 is also required, but not its deubiquitinating activity (PubMed : 17141156). Together with NSFL1C/p47, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase (PubMed : 23649807). Also, regulates spindle orientation during mitosis (PubMed : 23649807).
See full target information UBXN2B
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Complementary Products

Primary Antibodies

AB124032

Anti-UBXN2B antibody [OTI3H8]

Flow Cytometry (Intracellular) - Anti-UBXN2B antibody [OTI3H8] (AB124032)

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