Recombinant Human UCP2 protein is a Human Full Length protein, in the 1 to 309 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
Wheat germ
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
Amino acid sequence
M V G F K A T D V P P T A T V K F L G A G T A A C I A D L I T F P L G T A K V R L Q I Q G E S Q G P V R A T A S A Q Y R G V M G T I L T M V R T E G P R S L Y N G L V A G L Q R Q M S F A S V R I G L Y D S V K Q F Y T K G S E H A S I G S R L L A G S T T G A L A V A V A Q P T D V V K V R F Q A Q A R A G G G R R Y Q S T V N A Y K T I A R E E G F R G L W K G T S P N V A R N A I V N C A E L V T Y D L I K D A L L K A N L M T D D L P C H F T S A F G A G F C T T V I A S P V D V V K T R Y M N S A L G Q Y S S A G H C A L T M L Q K E G P R A F Y K G F M P S F L R L G S W N V V M F V T Y E Q L K R A L M A A C T S R E A P F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
Alternative names
Mitochondrial uncoupling protein 2, UCP 2, Solute carrier family 25 member 8, UCPH, UCP2, SLC25A8
Recommended products
Recombinant Human UCP2 protein is a Human Full Length protein, in the 1 to 309 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
Wheat germ
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
- Accession
- P55851-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
M V G F K A T D V P P T A T V K F L G A G T A A C I A D L I T F P L G T A K V R L Q I Q G E S Q G P V R A T A S A Q Y R G V M G T I L T M V R T E G P R S L Y N G L V A G L Q R Q M S F A S V R I G L Y D S V K Q F Y T K G S E H A S I G S R L L A G S T T G A L A V A V A Q P T D V V K V R F Q A Q A R A G G G R R Y Q S T V N A Y K T I A R E E G F R G L W K G T S P N V A R N A I V N C A E L V T Y D L I K D A L L K A N L M T D D L P C H F T S A F G A G F C T T V I A S P V D V V K T R Y M N S A L G Q Y S S A G H C A L T M L Q K E G P R A F Y K G F M P S F L R L G S W N V V M F V T Y E Q L K R A L M A A C T S R E A P F
- Accession
- Protein length
Full Length
- Predicted molecular weight
59.73 kDa
- Amino acids
1 to 309
- Nature
Recombinant
- Tags
GST tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
- Sequence similarities
Belongs to the mitochondrial carrier (TC 2.A.29) family.
- Subcellular localisation
Mitochondrion inner membrane
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
U+00A0;
U+00A0;
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UCP2 also known as Uncoupling Protein 2 is a mitochondrial protein that plays a role in regulating the production of heat by uncoupling oxidative phosphorylation from ATP synthesis. It has a molecular mass of approximately 33 kDa. UCP2 can be found in various tissues like the brain pancreas heart and adipose tissue. This protein facilitates the transport of protons across the inner mitochondrial membrane which dissipates the proton gradient leading to a reduction in ATP synthesis efficiency.
- Biological function summary
UCP2 influences energy balance and metabolism in cells. It is not part of a complex but it functions independently as a transporter within the mitochondrial membrane. UCP2 modulates reactive oxygen species (ROS) production impacting antioxidant defenses and influencing insulin secretion. Its role in decreasing ROS is particularly important for protecting cells from oxidative damage and helping in the maintenance of cellular homeostasis.
- Pathways
UCP2 is critical to thermogenesis and energy expenditure mechanisms. It is involved in the oxidative phosphorylation pathway affecting how cells produce energy. The protein is related to others in its family such as UCP1 and UCP3 which have similar functions but are expressed differently. UCP2 also intersects with pathways involving glucose metabolism where it influences insulin function and glucose uptake indirectly through its regulatory action.
- Associated diseases and disorders
UCP2 has links to obesity and diabetes. Its role in energy regulation and control of ROS offers insights into how imbalances can lead to metabolic syndromes. In obesity altered UCP2 expression may contribute to changes in energy expenditure and fat storage regulation. For diabetes UCP2 is closely connected to insulin signaling where its function impacts insulin secretion from pancreatic beta-cells. These connections underline the potential of UCP2 as a therapeutic target for metabolic diseases.
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1 product image
SDS-PAGE - Recombinant Human UCP2 protein (ab152783)
12.5% SDS-PAGE showing ab152783 stained with Coomassie Blue.
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