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AB107151

Recombinant Human UFC1 protein

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(1 Publication)

Recombinant Human UFC1 protein is a Human protein, in the 1 to 167 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

CGI-126, HSPC155, UFC1, Ubiquitin-fold modifier-conjugating enzyme 1, Ufm1-conjugating enzyme 1

2 Images
SDS-PAGE - Recombinant Human UFC1 protein (AB107151)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human UFC1 protein (AB107151)

15% SDS-PAGE showing ab107151 (3μg).

SDS-PAGE - Recombinant Human UFC1 protein (AB107151)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human UFC1 protein (AB107151)

SDS-PAGE showing ab107151

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9Y3C8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMADEATRRVVSEIPVLKTNAGPRDRELWVQRLKEEYQSLIRYVENNKNADNDWFRLESNKEGTRWFGKCWYIHDLLKYEFDIEFDIPITYPTTAPEIAVPELDGKTAKMYRGGKICLTDHFKPLWARNVPKFGLAHLMALGLGPWLAVEIPDLIQKGVIQHKEKCNQ","proteinLength":null,"predictedMolecularWeight":"21.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":167,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y3C8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UFC1 also known as ubiquitin-fold modifier-conjugating enzyme 1 or UFM1-conjugating enzyme 1 functions mechanically by facilitating the conjugation of ubiquitin-fold modifier 1 (UFM1) to target substrates. It exhibits a molecular mass of approximately 20.8 kDa. UFC1 is expressed in a variety of tissues including liver kidney and heart indicating its widespread role in cellular processes. As an essential component in the UFM1 system UFC1 works closely with other proteins to regulate protein modification through UFMylation.
Biological function summary

UFC1 is involved in the process of UFMylation which modifies proteins through the attachment of UFM1 much like ubiquitination influences protein degradation and signaling. UFC1 forms part of a complex that also includes UBA5 and UFL1 the E1 activating enzyme and E3 ligase respectively. This UFMylation system is necessary for endoplasmic reticulum-associated degradation (ERAD) contributing to the maintenance of protein homeostasis within cells. The disruption of this modification process can impact cellular stress response and protein quality control mechanisms.

Pathways

UFC1 plays a critical role in the ER stress response and protein quality control pathways. Within these pathways UFC1 interacts with proteins such as UFM1 and UFL1 to mediate the UFMylation of target proteins during cellular stress. Additionally UFC1 has been linked to pathways regulating autophagy where it indirectly influences the degradation of defective proteins. This interaction ultimately affects the cellular response to misfolded proteins and is pivotal for maintaining cellular equilibrium.

UFC1 has implications in conditions such as cancer and neurological disorders. Abnormalities in UFMylation can contribute to the dysregulation of protein homeostasis which is frequently observed in cancerous cells. In the context of neurological disorders the dysfunctional UFM1 system involving UFC1 can result in impaired neuronal homeostasis. Additionally UFC1's relationship with proteins like UFM1 and UFL1 highlights its importance in the pathological processes of these diseases positioning it as a potential target for therapeutic intervention.
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Specifications

Form

Liquid

Additional notes

ab107151 is purified using conventional chromatography techniques.

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General info

Function

E2-like enzyme which specifically catalyzes the second step in ufmylation (PubMed : 15071506, PubMed : 29868776, PubMed : 30626644, PubMed : 34588452, PubMed : 35394863, PubMed : 36121123, PubMed : 38383789). Accepts the ubiquitin-like modifier UFM1 from the E1 enzyme UBA5 and forms an intermediate with UFM1 via a thioester linkage (PubMed : 15071506, PubMed : 29868776, PubMed : 34588452, PubMed : 38383789). Ufmylation is involved in various processes, such as ribosome recycling, response to DNA damage, interferon response or reticulophagy (also called ER-phagy) (PubMed : 27351204, PubMed : 32160526, PubMed : 35394863, PubMed : 37036982, PubMed : 38383789).

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family. UFC1 subfamily.

Post-translational modifications

Ufmylated at Lys-122 (PubMed:35926457). Deufmylated by UFSP1 (PubMed:35926457).

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Product protocols

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Target data

E2-like enzyme which specifically catalyzes the second step in ufmylation (PubMed : 15071506, PubMed : 29868776, PubMed : 30626644, PubMed : 34588452, PubMed : 35394863, PubMed : 36121123, PubMed : 38383789). Accepts the ubiquitin-like modifier UFM1 from the E1 enzyme UBA5 and forms an intermediate with UFM1 via a thioester linkage (PubMed : 15071506, PubMed : 29868776, PubMed : 34588452, PubMed : 38383789). Ufmylation is involved in various processes, such as ribosome recycling, response to DNA damage, interferon response or reticulophagy (also called ER-phagy) (PubMed : 27351204, PubMed : 32160526, PubMed : 35394863, PubMed : 37036982, PubMed : 38383789).
See full target information UFC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

HIV medicine 16:114-21 PubMed25213431

2014

Autoantibodies binding to ubiquitin-fold modifier-conjugating enzyme 1 (Ufc1) and pleckstrin homology domain containing, family G (with RhoGef domain) member 2 (Plekhg2) are associated with mycobacterial infections.

Applications

Unspecified application

Species

Unspecified reactive species

A Tellermann,T Witte,C Lansche,M Stoll,R E Schmidt,N T Baerlecken
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