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Recombinant Human UFD1L protein is a Human Full Length protein, in the 1 to 307 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.

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Images

SDS-PAGE - Recombinant Human UFD1L protein (AB167871), expandable thumbnail

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, MS

Biologically active

No

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M G S M F S F N M F D H P I P R V F Q N R F S T Q Y R C F S V S M L A G P N D R S D V E K G G K I I M P P S A L D Q L S R L N I T Y P M L F K L T N K N S D R M T H C G V L E F V A D E G I C Y L P H W M M Q N L L L E E G G L V Q V E S V N L Q V A T Y S K F Q P Q S P D F L D I T N P K A V L E N A L R N F A C L T T G D V I A I N Y N E K I Y E L R V M E T K P D K A V S I I E C D M N V D F D A P L G Y K E P E R Q V Q H E E S T E G E A D H S G Y A G E L G F R A F S G S G N R L D G K K K G V E P S P S P I K P G D I K R G I P N Y E F K L G K I T F I R N S R P L V K K V E E D E A G G R F V A F S G E G Q S L R K K G R K P

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and NPLOC4, which binds to RIGI and recruits RNF125 to promote ubiquitination and degradation of RIGI (PubMed:26471729).

Alternative names

Recommended products

Recombinant Human UFD1L protein is a Human Full Length protein, in the 1 to 307 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, MS

Accession
Q92890-2
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris-HCl buffer, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Sequence info

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M G S M F S F N M F D H P I P R V F Q N R F S T Q Y R C F S V S M L A G P N D R S D V E K G G K I I M P P S A L D Q L S R L N I T Y P M L F K L T N K N S D R M T H C G V L E F V A D E G I C Y L P H W M M Q N L L L E E G G L V Q V E S V N L Q V A T Y S K F Q P Q S P D F L D I T N P K A V L E N A L R N F A C L T T G D V I A I N Y N E K I Y E L R V M E T K P D K A V S I I E C D M N V D F D A P L G Y K E P E R Q V Q H E E S T E G E A D H S G Y A G E L G F R A F S G S G N R L D G K K K G V E P S P S P I K P G D I K R G I P N Y E F K L G K I T F I R N S R P L V K K V E E D E A G G R F V A F S G E G Q S L R K K G R K P

Accession

Q92890

Protein length

Full Length

Predicted molecular weight

36.9 kDa

Amino acids

1 to 307

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

Additional notes

Purified by using conventional chromatography techniques.

General info

Function

Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and NPLOC4, which binds to RIGI and recruits RNF125 to promote ubiquitination and degradation of RIGI (PubMed:26471729).

Sequence similarities

Belongs to the UFD1 family.

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

UFD1L also known as Ubiquitin Fusion Degradation 1 Like is a protein with a molecular mass of approximately 35 kDa. It is expressed throughout most human tissues with higher levels observed in the brain heart and skeletal muscle. UFD1L plays an active role in the process of protein degradation by interacting with other molecules in the cell. This protein is part of the ubiquitin proteasome pathway and it partners with NPL4 and VCP/p97 making up a critical component of the ubiquitin-dependent degradation machinery.

Biological function summary

UFD1L interacts with cellular mechanisms for quality control by forming a complex with NPL4 and the ATPase VCP/p97. It functions to recognize and bind misfolded proteins earmarked for degradation thereby regulating protein homeostasis. This UFD1L complex assists in the extraction of ubiquitinated substrates from membranes and chromatin ensuring that only properly folded proteins are allowed to accumulate in cells which is essential for maintaining cellular function.

Pathways

UFD1L is integrally involved in the ER-associated degradation (ERAD) and the ubiquitin-proteasome pathway. In the ERAD pathway UFD1L works closely with VCP/p97 facilitating the removal of misfolded or excess proteins from the endoplasmic reticulum. It contributes to the regulation of the cell cycle and stress responses by ensuring efficient ubiquitin-dependent protein turnover. Its interaction with these pathways highlights its connection to a tightly regulated network of proteins including those involved in proteostasis and cellular stress regulation.

Associated diseases and disorders

UFD1L has been implicated in the pathogenesis of neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. The inappropriate function or expression of UFD1L and its associated proteins including VCP/p97 and NPL4 can lead to the accumulation of misfolded proteins a hallmark in these diseases. Further alterations in UFD1L are also associated with congenital disorders like DiGeorge syndrome linking it to critical developmental processes that go awry when protein degradation pathways are disrupted.

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1 product image

  • SDS-PAGE - Recombinant Human UFD1L protein (ab167871), expandable thumbnail

    SDS-PAGE - Recombinant Human UFD1L protein (ab167871)

    15% SDS-PAGE analysis of ab167871 (3µg). Note: Molecular size on SDS-PAGE will appear higher.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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