Recombinant Human UHRF1 protein is a Human Full Length protein, in the 2 to 793 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His-DDDDK tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M H H H H H H D Y K D D D D K W I Q V R T M D G R Q T H T V D S L S R L T K V E E L R R K I Q E L F H V E P G L Q R L F Y R G K Q M E D G H T L F D Y E V R L N D T I Q L L V R Q S L V L P H S T K E R D S E L S D T D S G C C L G Q S E S D K S S T H G E A A A E T D S R P A D E D M W D E T E L G L Y K V N E Y V D A R D T N M G A W F E A Q V V R V T R K A P S R D E P C S S T S R P A L E E D V I Y H V K Y D D Y P E N G V V Q M N S R D V R A R A R T I I K W Q D L E V G Q V V M L N Y N P D N P K E R G F W Y D A E I S R K R E T R T A R E L Y A N V V L G D D S L N D C R I I F V D E V F K I E R P G E G S P M V D N P M R R K S G P S C K H C K D D V N R L C R V C A C H L C G G R Q D P D K Q L M C D E C D M A F H I Y C L D P P L S S V P S E D E W Y C P E C R N D A S E V V L A G E R L R E S K K K A K M A S A T S S S Q R D W G K G M A C V G R T K E C T I V P S N H Y G P I P G I P V G T M W R F R V Q V S E S G V H R P H V A G I H G R S N D G A Y S L V L A G G Y E D D V D H G N F F T Y T G S G G R D L S G N K R T A E Q S C D Q K L T N T N R A L A L N C F A P I N D Q E G A E A K D W R S G K P V R V V R N V K G G K N S K Y A P A E G N R Y D G I Y K V V K Y W P E K G K S G F L V W R Y L L R R D D D E P G P W T K E G K D R I K K L G L T M Q Y P E G Y L E A L A N R E R E K E N S K R E E E E Q Q E G G F A S P R T G K G K W K R K S A G G G P S R A G S P R R T S K K T K V E P Y S L T A Q Q S S L I R E D K S N A K L W N E V L A S L K D R P A S G S P F Q L F L S K V E E T F Q C I C C Q E L V F R P I T T V C Q H N V C K D C L D R S F R A Q V F S C P A C R Y D L G R S Y A M Q V N Q P L Q T V L N Q L F P G Y G N G R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. Plays a role in DNA repair by cooperating with UHRF2 to ensure recruitment of FANCD2 to interstrand cross-links (ICLs) leading to FANCD2 activation. Acts as a critical player of proper spindle architecture by catalyzing the 'Lys-63'-linked ubiquitination of KIF11, thereby controlling KIF11 localization on the spindle (PubMed:37728657).
Alternative names
ICBP90, NP95, RNF106, UHRF1, E3 ubiquitin-protein ligase UHRF1, Inverted CCAAT box-binding protein of 90 kDa, Nuclear protein 95, Nuclear zinc finger protein Np95, RING finger protein 106, RING-type E3 ubiquitin transferase UHRF1, Transcription factor ICBP90, Ubiquitin-like PHD and RING finger domain-containing protein 1, Ubiquitin-like-containing PHD and RING finger domains protein 1, HuNp95, hNp95, hUHRF1
Recommended products
Recombinant Human UHRF1 protein is a Human Full Length protein, in the 2 to 793 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His-DDDDK tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q96T88-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M H H H H H H D Y K D D D D K W I Q V R T M D G R Q T H T V D S L S R L T K V E E L R R K I Q E L F H V E P G L Q R L F Y R G K Q M E D G H T L F D Y E V R L N D T I Q L L V R Q S L V L P H S T K E R D S E L S D T D S G C C L G Q S E S D K S S T H G E A A A E T D S R P A D E D M W D E T E L G L Y K V N E Y V D A R D T N M G A W F E A Q V V R V T R K A P S R D E P C S S T S R P A L E E D V I Y H V K Y D D Y P E N G V V Q M N S R D V R A R A R T I I K W Q D L E V G Q V V M L N Y N P D N P K E R G F W Y D A E I S R K R E T R T A R E L Y A N V V L G D D S L N D C R I I F V D E V F K I E R P G E G S P M V D N P M R R K S G P S C K H C K D D V N R L C R V C A C H L C G G R Q D P D K Q L M C D E C D M A F H I Y C L D P P L S S V P S E D E W Y C P E C R N D A S E V V L A G E R L R E S K K K A K M A S A T S S S Q R D W G K G M A C V G R T K E C T I V P S N H Y G P I P G I P V G T M W R F R V Q V S E S G V H R P H V A G I H G R S N D G A Y S L V L A G G Y E D D V D H G N F F T Y T G S G G R D L S G N K R T A E Q S C D Q K L T N T N R A L A L N C F A P I N D Q E G A E A K D W R S G K P V R V V R N V K G G K N S K Y A P A E G N R Y D G I Y K V V K Y W P E K G K S G F L V W R Y L L R R D D D E P G P W T K E G K D R I K K L G L T M Q Y P E G Y L E A L A N R E R E K E N S K R E E E E Q Q E G G F A S P R T G K G K W K R K S A G G G P S R A G S P R R T S K K T K V E P Y S L T A Q Q S S L I R E D K S N A K L W N E V L A S L K D R P A S G S P F Q L F L S K V E E T F Q C I C C Q E L V F R P I T T V C Q H N V C K D C L D R S F R A Q V F S C P A C R Y D L G R S Y A M Q V N Q P L Q T V L N Q L F P G Y G N G R
- Accession
- Q96T88
- Protein length
- Full Length
- Predicted molecular weight
- 92 kDa
- Amino acids
- 2 to 793
- Nature
- Recombinant
- Tags
- His-DDDDK tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. Plays a role in DNA repair by cooperating with UHRF2 to ensure recruitment of FANCD2 to interstrand cross-links (ICLs) leading to FANCD2 activation. Acts as a critical player of proper spindle architecture by catalyzing the 'Lys-63'-linked ubiquitination of KIF11, thereby controlling KIF11 localization on the spindle (PubMed:37728657).
- Post-translational modifications
Phosphorylation at Ser-298 of the linker region decreases the binding to H3K9me3. Phosphorylation at Ser-639 by CDK1 during M phase impairs interaction with USP7, preventing deubiquitination and leading to degradation by the proteasome.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UHRF1 also known as ICBP90 or Np95 is a nuclear protein playing key roles in epigenetic regulation. It weighs approximately 90 kDa. UHRF1 is expressed in various tissues most highly in proliferating cells. Functionally UHRF1 recognizes hemimethylated DNA and recruits DNA methyltransferase 1 (DNMT1) to maintain DNA methylation after replication. This ability to interact with both DNA and chromatin makes UHRF1 central to chromatin modification processes.
- Biological function summary
UHRF1 engages in preserving the DNA methylation landscape ensuring proper gene expression patterns. It is part of a multi-protein complex and processes both histone modification and DNA methylation. Its presence is critical for gene silencing through the recruitment of DNMT1 and histone deacetylases. UHRF1 therefore influences regulatory regions of genes important for cell cycle progression and development.
- Pathways
UHRF1 integrates into methylation-dependent gene silencing and cell cycle regulation. It actively participates in DNA damage repair pathways by modulating the p53 pathway aiding cell survival post-DNA damage. UHRF1 closely interacts with epigenetic regulators such as DNMT1 and histone deacetylases bridging methylation and chromatin remodeling to ensure proper cell cycle and transcriptional control.
- Associated diseases and disorders
UHRF1's dysregulation links to cancers including breast and lung cancer due to its role in maintaining aberrant methylation. Abnormal UHRF1 expression leads to improper silencing of tumor suppressor genes. UHRF1's interaction with DNMT1 and modulation of pathways involving p53 are important for cancer cell proliferation making it a potential target for cancer therapies.
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SDS-PAGE - Recombinant Human UHRF1 protein (ab198770)
4-20% SDS-PAGE analysis of ab198770 with Coomassie staining.
Lane 1: 2 μg ab198770
Lane 2: Protein marker
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