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AB125622

Recombinant human ULK2 protein (GST tag N-Terminus)

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Recombinant human ULK2 protein (GST tag N-Terminus) is a Human Fragment protein, in the 1 to 478 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, WB, FuncS.
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Functional Studies - Recombinant human ULK2 protein (GST tag N-Terminus) (AB125622)
  • FuncS

Unknown

Functional Studies - Recombinant human ULK2 protein (GST tag N-Terminus) (AB125622)

Sample Kinase Assay showing the specific activity of ab125622 as 102 nmol/min/mg.

Functional Studies - Recombinant human ULK2 protein (GST tag N-Terminus) (AB125622)
  • FuncS

Unknown

Functional Studies - Recombinant human ULK2 protein (GST tag N-Terminus) (AB125622)

The specific activity of ULK2 (ab125622) was determined to be 110.4 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human ULK2 protein (GST tag N-Terminus) (AB125622)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human ULK2 protein (GST tag N-Terminus) (AB125622)

SDS-PAGE analysis of ab125622.

SDS-PAGE - Recombinant human ULK2 protein (GST tag N-Terminus) (AB125622)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human ULK2 protein (GST tag N-Terminus) (AB125622)

SDS PAGE analysis of ab125622

Key facts

Purity

>80% Densitometry

Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE, WB

applications

Biologically active

Yes

Biological activity

The specific activity of ab125622 was determined to be 102 nmol/min/mg.

Accession

Q8IYT8-1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.004% EGTA, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Complementary Products

Primary Antibodies

AB97695

Anti-ULK2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ULK2 antibody (AB97695)

  • 2
  • 1
Primary Antibodies

AB138492

Anti-Collagen I antibody [EPR7785]

20ul selling size|BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [EPR7785] (AB138492)

  • 4
  • 37
Primary Antibodies

AB7778

Anti-Collagen III antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (AB7778)

  • 4
  • 24
Primary Antibodies

AB268020

Anti-Fibronectin antibody [EPR23110-46]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020)

  • 4
  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"linker":null,"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"98 kDa","actualMolecularWeight":null,"aminoAcidEnd":478,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q8IYT8","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ULK2 also known as UNC-51-like kinase 2 is a serine/threonine-protein kinase positioned within cellular processes. With an approximate molecular mass of 124 kDa ULK2 is expressed in various tissues prominently within the brain heart and skeletal muscle. This protein exhibits kinase activity essential for transferring phosphate groups to specific substrates affecting their function and activity.
Biological function summary

ULK2 functions as an important regulator in cellular autophagy the process that removes unnecessary or damaged cellular components. It acts as part of the ULK complex which includes ATG13 FIP200 and ATG101. This complex initiates the autophagy process by responding to nutrient availability and energy status within the cell effectively managing cellular homeostasis and survival.

Pathways

ULK2 is mainly involved in the autophagy pathway and the mTOR signaling pathway. The mTOR pathway influences cell growth and metabolism in response to nutritional signals hence ULK2 interacts with mTORC1 to relay signals important for appropriate autophagy initiation. Additionally ULK2 shares a functional relationship with AMPK which senses cellular energy levels and modulates autophagy in part through ULK2 activation.

ULK2 is associated with neurodegenerative diseases like Alzheimer's disease and can play a role in the pathology of certain cancers. Aberrant ULK2 activity alters autophagic processes contributing to the accumulation of misfolded proteins and impaired cell survival. The protein interacts with p62/SQSTM1 a known player in neurodegenerative disease pathways underlining its relevance to disease mechanisms.
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General info

Function

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy : acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation : may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

Post-translational modifications

Autophosphorylated. In response to nutrient limitation, probably phosphorylated and activated by AMPK, leading to activate autophagy.

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Product protocols

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Target data

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy : acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation : may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons.
See full target information ULK2

Alternative Names

KIAA0623, ULK2, Serine/threonine-protein kinase ULK2, Unc-51-like kinase 2
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