Recombinant human ULK3 protein
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Recombinant human ULK3 protein is a Human Full Length protein, expressed in Baculovirus infected insect cells, with >95%, suitable for SDS-PAGE, FuncS.
View Alternative Names
Serine/threonine-protein kinase ULK3, Unc-51-like kinase 3, ULK3
- FuncS
Unknown
Functional Studies - Recombinant human ULK3 protein (AB101548)
The specific activity of ULK3 (ab101548) was determined to be 210 nmol/min/mg as per activity assay protocol
- FuncS
Unknown
Functional Studies - Recombinant human ULK3 protein (AB101548)
Kinase Assay demonstrating specific activity of ab101548.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human ULK3 protein (AB101548)
SDS PAGE analysis of ab101548
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human ULK3 protein (AB101548)
SDS-PAGE showing ab101548 at approximately 51kDa.
Complementary Products
Primary Antibodies
AB124947
BOND RX™ Validated|RabMAb|Recombinant|KO Validated
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ULK3 antibody [EPR4888] (AB124947)](https://content.abcam.com/products/images/ulk3-antibody-epr4888-ab124947--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72645.jpg)
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- 0
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein contributes significantly through its involvement in autophagy regulation. ULK3 acts as a kinase within a multiprotein complex which includes proteins such as ATG13 and FIP200. This complex is essential for initiating autophagy by regulating the autophagosome formation. Its activity can promote cellular survival under nutrient-deprived conditions showcasing its role as an important regulatory component in cellular maintenance and stress responses.
Pathways
ULK3 takes part in critical signaling pathways such as the mTOR signaling pathway and the Hedgehog signaling pathway. In the mTOR pathway ULK3 through its kinase activity acts upstream to regulate the activity of the autophagy machinery. It shares a functional relationship with proteins like mTOR and AMPK. In the Hedgehog pathway ULK3 regulates pathway components impacting cellular growth and differentiation processes. This positions ULK3 as a modulator connecting different signaling pathways to maintain cellular homeostasis.
Specifications
Form
Liquid
Additional notes
The purity was determined to be >95% by densitometry. Affinity purified.
General info
Function
Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand : interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH : dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy : following cellular senescence, able to induce autophagy.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
Post-translational modifications
Autophosphorylated. Autophosphorylation is blocked by interaction with SUFU.
Target data
Product promise
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