Recombinant Human UNC119B protein is a Human Full Length protein, in the 1 to 251 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M S G S N P K A A A A A S A A G P G G L V A G K E E K K K A G G G V L N R L K A R R Q A P H H A A D D G V G A A V T E Q E L L A L D T I R P E H V L R L S R V T E N Y L C K P E D N I Y S I D F T R F K I R D L E T G T V L F E I A K P C V S D Q E E D E E E G G G D V D I S A G R F V R Y Q F T P A F L R L R T V G A T V E F T V G D K P V S N F R M I E R H Y F R E H L L K N F D F D F G F C I P S S R N T C E H I Y E F P Q L S E D V I R L M I E N P Y E T R S D S F Y F V D N K L I M H N K A D Y A Y N G G Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Myristoyl-binding protein that acts as a cargo adapter: specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. Binds myristoylated NPHP3 and plays a key role in localization of NPHP3 to the primary cilium membrane. Does not bind all myristoylated proteins. Probably plays a role in trafficking proteins in photoreceptor cells.
Alternative names
Protein unc-119 homolog B, UNC119B
Recommended products
Recombinant Human UNC119B protein is a Human Full Length protein, in the 1 to 251 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- A6NIH7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M S G S N P K A A A A A S A A G P G G L V A G K E E K K K A G G G V L N R L K A R R Q A P H H A A D D G V G A A V T E Q E L L A L D T I R P E H V L R L S R V T E N Y L C K P E D N I Y S I D F T R F K I R D L E T G T V L F E I A K P C V S D Q E E D E E E G G G D V D I S A G R F V R Y Q F T P A F L R L R T V G A T V E F T V G D K P V S N F R M I E R H Y F R E H L L K N F D F D F G F C I P S S R N T C E H I Y E F P Q L S E D V I R L M I E N P Y E T R S D S F Y F V D N K L I M H N K A D Y A Y N G G Q
- Accession
- A6NIH7
- Protein length
- Full Length
- Predicted molecular weight
- 30.3 kDa
- Amino acids
- 1 to 251
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab123153 was purified by using conventional chromatography techniques.
General info
- Function
Myristoyl-binding protein that acts as a cargo adapter: specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. Binds myristoylated NPHP3 and plays a key role in localization of NPHP3 to the primary cilium membrane. Does not bind all myristoylated proteins. Probably plays a role in trafficking proteins in photoreceptor cells.
- Sequence similarities
Belongs to the PDE6D/unc-119 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UNC119B also known as HRG4 is a protein involved in lipid-binding transport and plays an important role in the trafficking of lipidated proteins. The protein has a molecular mass of around 42 kDa and is commonly expressed in the retina and brain tissues. It binds myristoylated proteins facilitating their proper localization within cells. UNC119B interacts with specific proteins like ARL2 and ARL3 which are small GTP-binding proteins to perform its functions.
- Biological function summary
The ability of UNC119B to bind lipid-modified proteins plays a role in ensuring efficient protein trafficking within cells. It is part of a transport complex that delivers lipidated proteins to their correct cellular destinations. This function supports membrane dynamics by verifying that signaling molecules reach necessary cellular compartments. Interaction with ARL proteins helps stabilize the transport complex and facilitates its membrane association.
- Pathways
UNC119B functions significantly in the phototransduction and neuronal signaling pathways. It participates in the ciliary transport pathway which is important for the movement of proteins inside photoreceptor cells. UNC119B interacts with proteins like RPGR which is associated with proper functioning and maintenance of photoreceptor cells. This involvement is essential for preserving cell signaling and response mechanisms.
- Associated diseases and disorders
Research connects UNC119B with retinal degenerative diseases such as retinitis pigmentosa as well as neuronal disorders. Changes in UNC119B function impact the trafficking and signaling of photoreceptor cells associating it with these diseases. The protein’s interaction with RPGR is important as mutations in RPGR have links with retinal dystrophies. Understanding UNC119B's role in these diseases helps uncover potential therapeutic targets for treatment.
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1 product image
SDS-PAGE - Recombinant Human UNC119B protein (ab123153)
15% SDS-PAGE analysis of ab123153 (3 μg).
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