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AB167714

Recombinant Human uPA protein

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(3 Publications)

Recombinant Human uPA protein is a Human Full Length protein, in the 21 to 431 aa range, expressed in HEK 293 cells, with >92%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

Urokinase-type plasminogen activator, U-plasminogen activator, uPA, PLAU

2 Images
Functional Studies - Recombinant Human uPA protein (AB167714)
  • FuncS

Supplier Data

Functional Studies - Recombinant Human uPA protein (AB167714)

Immobilized ab167714 at 5 μg/mL (100 μL/well) can bind Biotinylated Human uPAR, His,Avitag with a linear range of 0.3-2 ng/mL

SDS-PAGE - Recombinant Human uPA protein (AB167714)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human uPA protein (AB167714)

SDS-PAGE of reduced ab167714 under reducing (R) condition, stained overnight with Coomassie Blue.

Key facts

Purity

>92% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

No

Accession

P00749

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

Storage buffer

pH: 7.4 Constituents: PBS, 0.41% Sodium acetate

storage-buffer

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Please note that the protein is not activated by protease digestion. In vitro activation is generally recommended for higher activity.
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Sequence info

[{"sequence":"SNELHQVPSNCDCLNGGTCVSNKYFSNIHWCNCPKKFGGQHCEIDKSKTCYEGNGHFYRGKASTDTMGRPCLPWNSATVLQQTYHAHRSDALQLGLGKHNYCRNPDNRRRPWCYVQVGLKPLVQECMVHDCADGKKPSSPPEELKFQCGQKTLRPRFKIIGGEFTTIENQPWFAAIYRRHRGGSVTYVCGGSLISPCWVISATHCFIDYPKKEDYIVYLGRSRLNSNTQGEMKFEVENLILHKDYSADTLAHHNDIALLKIRSKEGRCAQPSRTIQTICLPSMYNDPQFGTSCEITGFGKENSTDYLYPEQLKMTVVKLISHRECQQPHYYGSEVTTKMLCAADPQWKTDSCQGDSGGPLVCSLQGRMTLTGIVSWGRGCALKDKPGVYTRVSHFLPWIRSHTKEENGLAL","proteinLength":"Full Length","predictedMolecularWeight":"45 kDa","actualMolecularWeight":null,"aminoAcidEnd":431,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P00749","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Lyophilized

Additional notes

Less than 1.0 EU per µg by the LAL method.Purified by Immobilized metal affinity chromatography.

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General info

Function

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Sequence similarities

Belongs to the peptidase S1 family.

Post-translational modifications

Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.. Produced as an inactive single-chain protein (pro-uPA or sc-uPA), is processed into the active disulfide-linked two-chain form of PLAU/uPA by a proteolytic event mediated, at least, by TMPRSS4.

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Product protocols

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Target data

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
See full target information Urokinase-type plasminogen activator
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Lung cellular and molecular physiology 322:L593-L606 PubMed35200041

2022

Fibrinogen mediates cadmium-induced macrophage activation and serves as a predictor of cadmium exposure in chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Fu Jun Li,Ranu Surolia,Pooja Singh,Kevin G Dsouza,Crystal T Stephens,Zheng Wang,Rui-Ming Liu,Sejong Bae,Young-Il Kim,Mohammad Athar,Mark T Dransfield,Veena B Antony

Scientific reports 11:14849 PubMed34290311

2021

CD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-1.

Applications

Unspecified application

Species

Unspecified reactive species

Sibel Durlanik,Katrin Fundel-Clemens,Coralie Viollet,Heinrich J Huber,Martin Lenter,Kerstin Kitt,Stefan Pflanz

Reproduction (Cambridge, England) 160:853-862 PubMed33112787

2020

The cAMP-ERK1/2 signaling pathway regulates urokinase-type plasminogen activator-induced bovine granulosa cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Yufen Zhao,Boyang Yu,Xinyu Liu,Jitu Hu,Yanyan Yang,Erge Namei,Bingxue Yang,Yue Bai,Yinghong Qian,Haijun Li
View all publications
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Product promise

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