Skip to main content

Recombinant Human Urm1 protein is a Human Full Length protein, in the 1 to 101 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.

Be the first to review this product! Submit a review

Images

SDS-PAGE - Recombinant Human Urm1 protein (AB105598), expandable thumbnail

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, MS

Biologically active

No

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M A A P L S V E V E F G G G A E L L F D G I K K H R V T L P G Q E E P W D I R N L L I W I K K N L L K E R P E L F I Q G D S V R P G I L V L I N D A D W E L L G E L D Y Q L Q D Q D S V L F I S T L H G G

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.

Alternative names

Recommended products

Recombinant Human Urm1 protein is a Human Full Length protein, in the 1 to 101 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, MS

Accession
Q9BTM9-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Sequence info

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M A A P L S V E V E F G G G A E L L F D G I K K H R V T L P G Q E E P W D I R N L L I W I K K N L L K E R P E L F I Q G D S V R P G I L V L I N D A D W E L L G E L D Y Q L Q D Q D S V L F I S T L H G G

Accession

Q9BTM9

Protein length

Full Length

Predicted molecular weight

13.5 kDa

Amino acids

1 to 101

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

Additional notes

ab105598 was purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

General info

Function

Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.

Sequence similarities

Belongs to the URM1 family.

Post-translational modifications

C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Urm1 also known as ubiquitin-related modifier 1 is a small protein with a mass of approximately 11 kDa. It belongs to the ubiquitin-like protein family and is expressed in a wide range of tissues notably in human tissues at the cellular level. Urm1 functions as a molecular tag influencing a variety of cellular processes through its conjugation to substrate proteins. It shares structural similarities with ubiquitin and employs a similar conjugation pathway involving E1-like activating enzymes.

Biological function summary

Urm1 acts in the thiolation of tRNA and as part of the ubiquitin-related modifier system which affects protein modification and signaling. It plays roles in regulating sulfur metabolism and is important in the biosynthesis of thiolated tRNAs. Urm1 is also involved in oxidative stress response by being part of a complex that modifies proteins in reaction to environmental stressors. As a result Urm1 impacts cellular homeostasis and the stability of various proteins within the cell.

Pathways

Urm1 influences the tRNA modification pathway an important mechanism for proper protein synthesis and function. It also participates in the cellular response to oxidative stress primarily through its interaction with cellular signaling proteins such as ROS-responsive transcription factors. In these pathways Urm1-related conjugations modify proteins affecting their activity during cellular defense mechanisms and the maintenance of protein structure under stress conditions.

Associated diseases and disorders

Urm1 impacts conditions related to cellular redox states such as cancer and neurodegenerative disorders. Aberrant regulation of Urm1 and its pathways can lead to increased oxidative stress and faulty protein synthesis contributing to these diseases. Urm1's interaction with other proteins such as p53 in tumorigenesis highlights its relevance in the cellular processes that when deregulated can lead to cancer progression. In neurodegenerative diseases diminished Urm1 function can result in the accumulation of misfolded proteins exacerbating disease phenotypes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • SDS-PAGE - Recombinant Human Urm1 protein (ab105598), expandable thumbnail

    SDS-PAGE - Recombinant Human Urm1 protein (ab105598)

    15% SDS-PAGE analysis of 3μg ab105598.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com