Recombinant Human Uroplakin II/UPII protein (denatured) is a Human Fragment protein, in the 26 to 155 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S D F N I S S L S G L L S P A L T E S L L V A L P P C H L T G G N A T L M V R R A N D S K V V T S S F V V P P C R G R R E L V S V V D S G A G F T V T R L S A Y Q V T N L V P G T K F Y I S Y L V K K G T A T E S S R E I P M S T L P R R N M E S I G L G M A R T G G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in regulating the assembly of the AUM (By similarity).
Alternative names
Uroplakin-2, UP2, Uroplakin II, UPII, UPK2
Recommended products
Recombinant Human Uroplakin II/UPII protein (denatured) is a Human Fragment protein, in the 26 to 155 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O00526-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 12.01% Urea, 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S D F N I S S L S G L L S P A L T E S L L V A L P P C H L T G G N A T L M V R R A N D S K V V T S S F V V P P C R G R R E L V S V V D S G A G F T V T R L S A Y Q V T N L V P G T K F Y I S Y L V K K G T A T E S S R E I P M S T L P R R N M E S I G L G M A R T G G
- Accession
- O00526
- Protein length
- Fragment
- Predicted molecular weight
- 16.2 kDa
- Amino acids
- 26 to 155
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in regulating the assembly of the AUM (By similarity).
- Sequence similarities
Belongs to the uroplakin-2 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as Uroplakin II.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Uroplakin II (UPII) also known as UPK2 plays a structural role in the epithelial cells of the urinary tract. It has a molecular mass of approximately 15 kDa. UPII is expressed specifically in the urothelium which lines the interior surface of the bladder ureters and renal pelvis. This protein contributes to the asymmetric unit membrane (AUM) which forms specialized plaques essential for maintaining the impermeability of the urothelial barrier.
- Biological function summary
Uroplakin II functions as part of the larger uroplakin protein complex. UPII interacts with other uroplakins such as Uroplakin Ia Ib and III contributing to the formation of a multimeric structure that strengthens the urothelial cells' surface. This structure plays a role in cellular stabilization and preventing the passage of toxic substances and pathogens from the urine into the underlying tissue. Consequently it aids in maintaining tissue integrity and ensuring efficient functioning of the urinary system.
- Pathways
The uroplakin complex integrates into the pathway of epithelial differentiation. Uroplakin II's association with other uroplakins influences the formation of the AUM which involves the coordinated action of several related proteins such as claudins. The interaction with claudins suggests a potential regulatory role for cell-cell adhesion within epithelial transport pathways. This role might interact with tight junction pathways that are important for maintaining the selective permeability of epithelial layers.
- Associated diseases and disorders
Alterations in Uroplakin II expression link to urothelial carcinoma. Abnormal UPII levels can disrupt the urothelial barrier potentially leading to carcinogenesis. Investigations find connections between UPII and overexpression of proteins such as EGFR which contributes to tumor growth and progression. The association of UPII with bladder cancer emphasizes its potential as a diagnostic biomarker and a target for therapeutic strategies in urothelial malignancies.
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1 product image
SDS-PAGE - Recombinant Human Uroplakin II/UPII protein (denatured) (ab139621)
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
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