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AB269114

Recombinant human USP11 protein (Tagged)

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(1 Publication)

Recombinant human USP11 protein (Tagged) is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE and Functional studies. The predicted molecular weight of ab269114 protein is 136 kDa.

- Save time and ensure accurate results - use our human USP11 protein (GST-tag) as a control
- Optimal protein bioactivity and stability

View Alternative Names

UHX1, USP11, Ubiquitin carboxyl-terminal hydrolase 11, Deubiquitinating enzyme 11, Ubiquitin thioesterase 11, Ubiquitin-specific-processing protease 11

2 Images
Functional Studies - Recombinant human USP11 protein (Tagged) (AB269114)
  • FuncS

Supplier Data

Functional Studies - Recombinant human USP11 protein (Tagged) (AB269114)

The specific activity of ab269114 was determined to be 47 nmol/min/mg in a DUB assay using ubiquitin-based proluciferin substrate.

SDS-PAGE - Recombinant human USP11 protein (Tagged) (AB269114)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human USP11 protein (Tagged) (AB269114)

SDS-PAGE gel of ab269114.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity of ab269114 was determined to be 47 nmol/min/mg in a DUB assay using recombinant human ubiquitin-based proluciferin substrate.

Accession

P51784

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Ensure the validity of your result using our bioactive recombinant human USP11 protein (GST-Tagged) ab269114 as a control in SDS-PAGE.


Check out our protein gel staining guide for SDS-PAGE here
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":"136 kDa","aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P51784","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP11 also known as ubiquitin specific peptidase 11 is a deubiquitinating enzyme with a molecular mass of approximately 110 kDa. It removes ubiquitin molecules from target proteins regulating their stability and function. This enzyme is part of the USP family and exhibits strong expression in tissues like the testes brain and thyroid. The deubiquitination process is significant in maintaining protein homeostasis by recycling ubiquitins and controlling protein degradation.
Biological function summary

Ubiquitin-specific peptidases such as USP11 participate in diverse cellular functions including DNA repair signal transduction and cell cycle progression. USP11 often operates as part of a larger regulatory complex. It modulates DNA repair by interacting with proteins involved in the repair processes such as BRCA2. Through its deubiquitination activity USP11 significantly influences cellular response to DNA damage and helps to maintain genomic stability.

Pathways

USP11 plays a role in key mechanisms like the DNA damage response and NF-kB signaling pathways. It interacts with proteins involved in these pathways offering a layer of regulation. In the DNA damage response pathway USP11 associates with BRCA2 assisting in the repair of double-stranded breaks. In the NF-kB signaling pathway its activity can affect the degradation of IkB which allows the transcription factor NF-kB to enter the nucleus and promote gene expression.

Aberrations in USP11 function have been linked to cancer and neurodegenerative diseases. In cancer alterations in USP11 might contribute to tumorigenesis by affecting the DNA repair pathways especially through the BRCA2 protein interactions. Neurodegenerative diseases may also be connected to USP11 dysfunction due to its role in the degradation of proteins that accumulate in these conditions. The enzyme's interaction with various signaling proteins suggests a potential target for therapeutic interventions in these diseases.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains (PubMed : 12084015, PubMed : 15314155, PubMed : 17897950, PubMed : 19874889, PubMed : 20233726, PubMed : 24724799, PubMed : 28992046). Inhibits the degradation of target proteins by the proteasome (PubMed : 12084015). Cleaves preferentially 'Lys-6' and 'Lys-63'-linked ubiquitin chains. Has lower activity with 'Lys-11' and 'Lys-33'-linked ubiquitin chains, and extremely low activity with 'Lys-27', 'Lys-29' and 'Lys-48'-linked ubiquitin chains (in vitro) (PubMed : 24724799). Plays a role in the regulation of pathways leading to NF-kappa-B activation (PubMed : 17897950, PubMed : 19874889). Plays a role in the regulation of DNA repair after double-stranded DNA breaks (PubMed : 15314155, PubMed : 20233726). Acts as a chromatin regulator via its association with the Polycomb group (PcG) multiprotein PRC1-like complex; may act by deubiquitinating components of the PRC1-like complex (PubMed : 20601937). Promotes cell proliferation by deubiquitinating phosphorylated E2F1 (PubMed : 28992046).

Sequence similarities

Belongs to the peptidase C19 family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains (PubMed : 12084015, PubMed : 15314155, PubMed : 17897950, PubMed : 19874889, PubMed : 20233726, PubMed : 24724799, PubMed : 28992046). Inhibits the degradation of target proteins by the proteasome (PubMed : 12084015). Cleaves preferentially 'Lys-6' and 'Lys-63'-linked ubiquitin chains. Has lower activity with 'Lys-11' and 'Lys-33'-linked ubiquitin chains, and extremely low activity with 'Lys-27', 'Lys-29' and 'Lys-48'-linked ubiquitin chains (in vitro) (PubMed : 24724799). Plays a role in the regulation of pathways leading to NF-kappa-B activation (PubMed : 17897950, PubMed : 19874889). Plays a role in the regulation of DNA repair after double-stranded DNA breaks (PubMed : 15314155, PubMed : 20233726). Acts as a chromatin regulator via its association with the Polycomb group (PcG) multiprotein PRC1-like complex; may act by deubiquitinating components of the PRC1-like complex (PubMed : 20601937). Promotes cell proliferation by deubiquitinating phosphorylated E2F1 (PubMed : 28992046).
See full target information USP11
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer communications (London, England) 42:1008-1027 PubMed35975322

2022

A non-metabolic function of hexokinase 2 in small cell lung cancer: promotes cancer cell stemness by increasing USP11-mediated CD133 stability.

Applications

Unspecified application

Species

Unspecified reactive species

Juhong Wang,Fei Shao,Yannan Yang,Wei Wang,Xueying Yang,Renda Li,Hong Cheng,Sijin Sun,Xiaoli Feng,Yibo Gao,Jie He,Zhimin Lu
View all publications
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