Recombinant Human USP12 protein
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Recombinant Human USP12 protein is a Human Fragment protein, in the 165 to 370 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.
View Alternative Names
UBH1, USP12L1, USP12, Ubiquitin carboxyl-terminal hydrolase 12, Deubiquitinating enzyme 12, Ubiquitin specific peptidase 12, Ubiquitin thioesterase 12, Ubiquitin-hydrolyzing enzyme 1, Ubiquitin-specific-processing protease 12
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
USP12 regulates protein degradation and turnover in cells affecting pathways involved in maintaining protein homeostasis. It forms a complex with WDR20 and UAF1 enhancing its enzymatic activity. This complex allows for precise regulation of substrate specificity and function in various cellular contexts. Through its deubiquitinating activity USP12 plays a role in cellular signaling and stress response.
Pathways
USP12 participates in the ubiquitin-proteasome pathway and the DNA damage response pathway. Its activity influences the turnover of proteins that participate in these processes such as androgen receptor and MDM2. The interaction with these proteins highlights USP12's involvement in pathways that are important for cellular responses to damage and the regulation of gene expression.
Specifications
Form
Lyophilized
Additional notes
Purified via His tag
General info
Function
Deubiquitinating enzyme that plays various roles in the regulation of the immune response and inflammation (PubMed : 19075014, PubMed : 27373336). During TCR engagement and activation, translocates into the cytoplasm and deubiquitinates its substrates LAT and TRAT1 and prevents their lysosome-dependent degradation to stabilize the TCR signaling complex at the plasma membrane (PubMed : 26811477). Plays an essential role in the selective LPS-induced macrophage response through the activation of NF-kappa-B pathway (PubMed : 28063927). In addition, promotes that antiviral immune response through targeting DNA sensor IFI16 to inhibit its proteasome-dependent degradation (PubMed : 37410794). Participates in the interferon signaling pathway and antiviral response independently of its deubiquitinase activity by maintaining nuclear phosphorylated STAT1 levels via inhibition of its CREBBP-mediated acetylation and subsequent dephosphorylation (PubMed : 31899788). Plays an intrinsic role in promoting the differentiation, activation and proliferation of CD4(+) T-cell by activating the NF-kappa-B signaling pathway through deubiquitinating and stabilizing B-cell lymphoma/leukemia 10/BCL10 (By similarity). In myeloid-derived suppressor cells promotes the activation of the NF-kappa-B via deubiquitination and stabilization of RELA (By similarity). Regulates the 'Lys-63'-linked polyubiquitin chains of BAX and thereby modulates the mitochondrial apoptotic process (PubMed : 36361894). Negative regulator of NOTCH signaling that specifically deubiquitinates non-activated NOTCH receptors to target them for lysosomal degradation; deubiquitination of NOTCH stimulates its transport form late endosomes to lysosomes (PubMed : 22778262). Protects neurons against HTT/huntingtin-induced polyglutamine expansion-dependent neurodegeneration through regulation of autophagic flux (PubMed : 30266909). This function is independent of deubiquitinase activity or of other components of the USP12-WDR20-WDR48 deubiquitinating complex (By similarity). In complex with WDR48, acts as a potential tumor suppressor by positively regulating PHLPP1 stability (PubMed : 24145035).. (Microbial infection) Forms a complex with Epstein-Barr virus protein EBNA3 which is an active deubiquitinase activity that may select specific substrates to promote B-lymphocyte transformation.
Sequence similarities
Belongs to the peptidase C19 family. USP12/USP46 subfamily.
Target data
Complementary Products
Product promise
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