Recombinant human USP14/TGT protein is a Human Full Length protein, in the 2 to 494 aa range, expressed in Baculovirus infected Sf9, with >=75% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >=75% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
P L Y S V T V K W G K E K F E G V E L N T D E P P M V F K A Q L F A L T G V Q P A R Q K V M V K G G T L K D D D W G N I K I K N G M T L L M M G S A D A L P E E P S A K T V F V E D M T E E Q L A S A M E L P C G L T N L G N T C Y M N A T V Q C I R S V P E L K D A L K R Y A G A L R A S G E M A S A Q Y I T A A L R D L F D S M D K T S S S I P P I I L L Q F L H M A F P Q F A E K G E Q G Q Y L Q Q D A N E C W I Q M M R V L Q Q K L E A I E D D S V K E T D S S S A S A A T P S K K K S L I D Q F F G V E F E T T M K C T E S E E E E V T K G K E N Q L Q L S C F I N Q E V K Y L F T G L K L R L Q E E I T K Q S P T L Q R N A L Y I K S S K I S R L P A Y L T I Q M V R F F Y K E K E S V N A K V L K D V K F P L M L D M Y E L C T P E L Q E K M V S F R S K F K D L E D K K V N Q Q P N T S D K K S S P Q K E V K Y E P F S F A D D I G S N N C G Y Y D L Q A V L T H Q G R S S S S G H Y V S W V K R K Q D E W I K F D D D K V S I V T P E D I L R L S G G G D W H I A Y V L L Y G P R R V E I M E E E S E Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins (PubMed:35145029). Ensures the regeneration of ubiquitin at the proteasome (PubMed:18162577, PubMed:28396413). Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell (PubMed:18162577). Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (PubMed:19106094). Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1 (PubMed:19135427). Indispensable for synaptic development and function at neuromuscular junctions (NMJs) (By similarity). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation (PubMed:27666593). Inhibits OPTN-mediated selective autophagic degradation of KDM4D and thereby negatively regulates H3K9me2 and H3K9me3 (PubMed:35145029).
Alternative names
TGT, USP14, Ubiquitin carboxyl-terminal hydrolase 14, Deubiquitinating enzyme 14, Ubiquitin thioesterase 14, Ubiquitin-specific-processing protease 14
Recommended products
Recombinant human USP14/TGT protein is a Human Full Length protein, in the 2 to 494 aa range, expressed in Baculovirus infected Sf9, with >=75% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=75% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: 56 pmol/min/μg.
Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP14 in a 50 μL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.
- Accession
- P54578-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.72% Sodium chloride, 0.71% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- P L Y S V T V K W G K E K F E G V E L N T D E P P M V F K A Q L F A L T G V Q P A R Q K V M V K G G T L K D D D W G N I K I K N G M T L L M M G S A D A L P E E P S A K T V F V E D M T E E Q L A S A M E L P C G L T N L G N T C Y M N A T V Q C I R S V P E L K D A L K R Y A G A L R A S G E M A S A Q Y I T A A L R D L F D S M D K T S S S I P P I I L L Q F L H M A F P Q F A E K G E Q G Q Y L Q Q D A N E C W I Q M M R V L Q Q K L E A I E D D S V K E T D S S S A S A A T P S K K K S L I D Q F F G V E F E T T M K C T E S E E E E V T K G K E N Q L Q L S C F I N Q E V K Y L F T G L K L R L Q E E I T K Q S P T L Q R N A L Y I K S S K I S R L P A Y L T I Q M V R F F Y K E K E S V N A K V L K D V K F P L M L D M Y E L C T P E L Q E K M V S F R S K F K D L E D K K V N Q Q P N T S D K K S S P Q K E V K Y E P F S F A D D I G S N N C G Y Y D L Q A V L T H Q G R S S S S G H Y V S W V K R K Q D E W I K F D D D K V S I V T P E D I L R L S G G G D W H I A Y V L L Y G P R R V E I M E E E S E Q
- Accession
- P54578
- Protein length
- Full Length
- Predicted molecular weight
- 57 kDa
- Amino acids
- 2 to 494
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins (PubMed:35145029). Ensures the regeneration of ubiquitin at the proteasome (PubMed:18162577, PubMed:28396413). Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell (PubMed:18162577). Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (PubMed:19106094). Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1 (PubMed:19135427). Indispensable for synaptic development and function at neuromuscular junctions (NMJs) (By similarity). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation (PubMed:27666593). Inhibits OPTN-mediated selective autophagic degradation of KDM4D and thereby negatively regulates H3K9me2 and H3K9me3 (PubMed:35145029).
- Sequence similarities
Belongs to the peptidase C19 family. USP14/UBP6 subfamily.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product was previously labelled as USP14
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
USP14 also known as Ubiquitin Specific Peptidase 14 is a deubiquitinating enzyme that plays a mechanical role in the ubiquitin-proteasome system. This enzyme has a molecular mass around 56 kDa and is essential for proteasome-mediated degradation activities. It is broadly expressed across many tissues but shows significant presence in the nervous system. Often people refer to USP14 as TGT in the context of certain studies or databases.
- Biological function summary
USP14 functions in regulating protein turnover by removing ubiquitin from substrate proteins impacting their stability and degradation. USP14 is part of a larger protein complex associated with the proteasome specifically binding to the 19S regulatory particle. It is important in maintaining cellular protein homeostasis by editing ubiquitin chains on target proteins prior to degradation.
- Pathways
USP14 participates in ubiquitin-proteasome pathways and ER-associated degradation pathway. In these processes it works closely with other proteasome proteins such as Rpn11 which also removes ubiquitin from proteins. The regulation of these pathways by USP14 directly affects protein quality control in cells influencing processes like cell cycle and stress response.
- Associated diseases and disorders
USP14 has associations with neurodegenerative diseases such as Alzheimer's and Parkinson’s. It affects disease progression by modulating levels of proteins that aggregate in these conditions. Connections between USP14 and proteins such as tau and α-synuclein highlight its contribution to the pathological features seen in these disorders. Further research into USP14's role may help to uncover new therapeutic strategies for these diseases.
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2 product images
SDS-PAGE - Recombinant human USP14/TGT protein (ab198432)
10% SDS-PAGE Coomassie staining using 2.8 μg ab198432 (Lane 1). Lane 2: Protein Marker
Functional Studies - Recombinant human USP14/TGT protein (ab198432)
Activity assay using ab198432.
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