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AB269116

Recombinant human USP15 protein (Active)

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Recombinant human USP15 protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

KIAA0529, USP15, Ubiquitin carboxyl-terminal hydrolase 15, Deubiquitinating enzyme 15, Ubiquitin thioesterase 15, Ubiquitin-specific-processing protease 15, Unph-2, Unph4

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Functional Studies - Recombinant human USP15 protein (Active) (AB269116)
  • FuncS

Supplier Data

Functional Studies - Recombinant human USP15 protein (Active) (AB269116)

The specific activity of ab269116 was ≥121 nmol/min/mg in a DUB assay using recombinant ubiquitin-based proluciferin as substrate.

SDS-PAGE - Recombinant human USP15 protein (Active) (AB269116)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human USP15 protein (Active) (AB269116)

SDS-PAGE analysis of ab269116.

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab269116 was ≥ 121 nmol/min/mg in a DUB assay using recombinant ubiquitin-based proluciferin as substrate

Accession

Q9Y4E8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

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Complementary Products

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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Primary Antibodies

AB256453

Anti-Perforin antibody [EPR22829-42]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-Perforin antibody [EPR22829-42] (AB256453)

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Proteins & Peptides

AB139775

Recombinant Human USP14/TGT protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human USP14/TGT protein (His tag N-Terminus) (AB139775)

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Proteins & Peptides

AB269138

Recombinant human Wwp2 protein (Active)

Functional Studies - Recombinant human Wwp2 protein (Active) (AB269138)

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Primary Antibodies

AB71713

Anti-USP15 antibody

Immunoprecipitation - Anti-USP15 antibody (AB71713)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9Y4E8","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP15 also known as Ubiquitin Specific Peptidase 15 is a deubiquitinating enzyme that removes ubiquitin from target proteins a process integral to protein degradation and regulation. It has a molecular mass of approximately 107 kDa. USP15 regulates protein turnover and stability through its enzymatic activity and is predominantly cytoplasmic expressed in various tissues including the brain heart and liver. Its expression pattern suggests widespread functionality across different physiological contexts.
Biological function summary

USP15 is involved in numerous cellular processes through its role in protein homeostasis. It associates with several protein complexes to regulate signaling pathways and cellular responses. USP15 plays a central role in the regulation of TGF-β signaling by deubiquitinating and stabilizing SMAD proteins that are important for signal transduction. In addition to TGF-β modulation USP15 also participates in endocytic trafficking by associating with the ESCRT complex impacting receptor degradation and cell signaling.

Pathways

The function of USP15 is critical in integrating cellular signaling networks such as the TGF-β and NF-kB pathways. Within the TGF-β pathway USP15 deubiquitinates receptor-regulated SMADs maintaining their stability and enhancing signal transduction. In the NF-kB pathway USP15 influences the activation by regulating the degradation of IκBα an inhibitor of NF-kB which indicates its role in immune response and inflammation. Through these pathways USP15 interacts with proteins like SMAD2/3 in TGF-β signaling and IκBα in NF-kB regulation.

USP15 has associations with cancer and neurodegenerative diseases. In cancer altered USP15 activity leads to dysregulation of TGF-β signaling influencing tumor progression and metastasis. The enzyme interacts with proteins such as MDM2 in neurodegenerative disorders affecting p53 stability and neuronal cell survival. This suggests a role in processes like apoptosis and DNA repair critical in the onset and progression of neurodegenerative conditions. But current research continues aiming for better understanding of USP15's connections with these disorders.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling, NF-kappa-B and RNF41/NRDP1-PRKN pathways (PubMed : 16005295, PubMed : 17318178, PubMed : 19576224, PubMed : 19826004, PubMed : 21947082, PubMed : 22344298, PubMed : 24852371). Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear : according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes (PubMed : 21947082). According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of TGFBR1, leading to an enhanced TGF-beta signal (PubMed : 22344298). Able to mediate deubiquitination of monoubiquitinated substrates, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 33093067). May also regulate gene expression and/or DNA repair through the deubiquitination of histone H2B (PubMed : 24526689). Acts as an inhibitor of mitophagy by counteracting the action of parkin (PRKN) : hydrolyzes cleavage of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains attached by parkin on target proteins such as MFN2, thereby reducing parkin's ability to drive mitophagy (PubMed : 24852371). Acts as an associated component of COP9 signalosome complex (CSN) and regulates different pathways via this association : regulates NF-kappa-B by mediating deubiquitination of NFKBIA and deubiquitinates substrates bound to VCP (PubMed : 16005295, PubMed : 17318178, PubMed : 19576224, PubMed : 19826004). Involved in endosome organization by mediating deubiquitination of SQSTM1 : ubiquitinated SQSTM1 forms a molecular bridge that restrains cognate vesicles in the perinuclear region and its deubiquitination releases target vesicles for fast transport into the cell periphery (PubMed : 27368102). Acts as a negative regulator of antifungal immunity by mediating 'Lys-27'-linked deubiquitination of CARD9, thereby inactivating CARD9 (PubMed : 33093067).. (Microbial infection) Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.

Sequence similarities

Belongs to the peptidase C19 family.

Post-translational modifications

Phosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome.. Ubiquitinated, leading to degradation by the proteasome.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling, NF-kappa-B and RNF41/NRDP1-PRKN pathways (PubMed : 16005295, PubMed : 17318178, PubMed : 19576224, PubMed : 19826004, PubMed : 21947082, PubMed : 22344298, PubMed : 24852371). Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear : according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes (PubMed : 21947082). According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of TGFBR1, leading to an enhanced TGF-beta signal (PubMed : 22344298). Able to mediate deubiquitination of monoubiquitinated substrates, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 33093067). May also regulate gene expression and/or DNA repair through the deubiquitination of histone H2B (PubMed : 24526689). Acts as an inhibitor of mitophagy by counteracting the action of parkin (PRKN) : hydrolyzes cleavage of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains attached by parkin on target proteins such as MFN2, thereby reducing parkin's ability to drive mitophagy (PubMed : 24852371). Acts as an associated component of COP9 signalosome complex (CSN) and regulates different pathways via this association : regulates NF-kappa-B by mediating deubiquitination of NFKBIA and deubiquitinates substrates bound to VCP (PubMed : 16005295, PubMed : 17318178, PubMed : 19576224, PubMed : 19826004). Involved in endosome organization by mediating deubiquitination of SQSTM1 : ubiquitinated SQSTM1 forms a molecular bridge that restrains cognate vesicles in the perinuclear region and its deubiquitination releases target vesicles for fast transport into the cell periphery (PubMed : 27368102). Acts as a negative regulator of antifungal immunity by mediating 'Lys-27'-linked deubiquitination of CARD9, thereby inactivating CARD9 (PubMed : 33093067).. (Microbial infection) Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.
See full target information USP15
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