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AB269117

Recombinant human USP16 protein (Active)

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Recombinant human USP16 protein (Active) is a Human Fragment protein, in the 187 to 823 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, FuncS.

View Alternative Names

MSTP039, USP16, Ubiquitin carboxyl-terminal hydrolase 16, Deubiquitinating enzyme 16, Ubiquitin thioesterase 16, Ubiquitin-processing protease UBP-M, Ubiquitin-specific-processing protease 16

2 Images
Functional Studies - Recombinant human USP16 protein (Active) (AB269117)
  • FuncS

Supplier Data

Functional Studies - Recombinant human USP16 protein (Active) (AB269117)

The specific activity of ab269117 was 24 nmol/min/mg in a DUB assay using ubiquitin-based proluciferin substrate.

SDS-PAGE - Recombinant human USP16 protein (Active) (AB269117)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human USP16 protein (Active) (AB269117)

Recombinant human USP16 protein (ab269117) resolved on a SDS-PAGE gel.

Key facts

Purity

>85% SDS-PAGE

Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab269117 was 24 nmol/min/mg in a DUB assay using ubiquitin-based proluciferin substrate.

Accession

Q9Y5T5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Recombinant human WNK3 protein (Active)

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Recombinant Human USP14/TGT protein (His tag N-Terminus)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Proteins & Peptides

AB198464

Recombinant human HAUSP / USP7 protein (His-DDDDK tag N-Terminus)

Functional Studies - Recombinant human HAUSP / USP7 protein (His-DDDDK tag N-Terminus) (AB198464)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MNSPCQITVKGLSNLGNTCFFNAVMQNLSQTPVLRELLKEVKMSGTIVKIEPPDLALTEPLEINLEPPGPLTLAMSQFLNEMQETKKGVVTPKELFSQVCKKAVRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFGNSTEKLDEELKNKVKDYEKKKSMPSFVDRIFGGELTSMIMCDQCRTVSLVHESFLDLSLPVLDDQSGKKSVNDKNLKKTVEDEDQDSEEEKDNDSYIKERSDIPSGTSKHLQKKAKKQAKKQAKNQRRQQKIQGKVLHLNDICTIDHPEDSEYEAEMSLQGEVNIKSNHISQEGVMHKEYCVNQKDLNGQAKMIESVTDNQKSTEEVDMKNINMDNDLEVLTSSPTRNLNGAYLTEGSNGEVDISNGFKNLNLNAALHPDEINIEILNDSHTPGTKVYEVVNEDPETAFCTLANREVFNTDECSIQHCLYQFTRNEKLRDANKLLCEVCTRRQCNGPKANIKGERKHVYTNAKKQMLISLAPPVLTLHLKRFQQAGFNLRKVNKHIKFPEILDLAPFCTLKCKNVAEENTRVLYSLYGVVEHSGTMRSGHYTAYAKARTANSHLSNLVLHGDIPQDFEMESKGQWFHISDTHVQAVPTTKVLNSQAYLLFYERIL","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":823,"aminoAcidStart":187,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9Y5T5","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP16 also known as Ubiquitin Specific Peptidase 16 functions as a deubiquitinating enzyme that regulates protein degradation by cleaving ubiquitin from ubiquitin-conjugated protein substrates. It has a molecular mass of approximately 97 kDa. USP16 is expressed in various tissues with high levels in the brain and heart. It has an integral role in maintaining protein homeostasis by regulating degradation pathways necessary for cellular function.
Biological function summary

The enzymatic activity of USP16 removes ubiquitin moieties contributing to the stabilization of target proteins. It participates in several cellular processes including cell cycle regulation DNA damage response and chromatin remodeling. USP16 is associated with the Polycomb group (PcG) complex which plays a role in transcriptional repression. This involvement in epigenetic regulation highlights its importance in gene expression and cellular differentiation.

Pathways

USP16 is significant in the Wnt signaling pathway and the Hedgehog signaling pathway. In the Wnt pathway USP16 regulates beta-catenin degradation influencing cell proliferation and differentiation. Through the Hedgehog pathway USP16 impacts the stability of GLI transcription factors affecting developmental processes and tissue patterning. USP16 interacts with proteins like Axin and APC in the Wnt pathway illustrating its role in complex regulatory networks.

Researchers have found connections between USP16 dysregulation and cancer particularly leukemia and neurodegenerative diseases like Alzheimer's disease. In cancer USP16 overexpression can lead to increased cell proliferation through pathways involving proteins such as MYC and Cyclin D1. Related to neurodegenerative disorders USP16's interaction with proteins like amyloid precursor protein (APP) contributes to pathologies like Alzheimer's where proteostasis and ubiquitination play critical roles. Understanding USP16's functions and connections can provide insights into therapeutic targets for these diseases.
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General info

Function

Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator (PubMed : 17914355). Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis (PubMed : 17914355). In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination (PubMed : 17914355). Prefers nucleosomal substrates (PubMed : 17914355). Does not deubiquitinate histone H2B (PubMed : 17914355). Also deubiquitinates non-histone proteins, such as ribosomal protein RPS27A : deubiquitination of monoubiquitinated RPS27A promotes maturation of the 40S ribosomal subunit (PubMed : 32129764). Also mediates deubiquitination of tektin proteins (TEKT1, TEKT2, TEK3, TEKT4 and TEKT5), promoting their stability.

Sequence similarities

Belongs to the peptidase C19 family. USP16 subfamily.

Post-translational modifications

Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity.

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Product protocols

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Target data

Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator (PubMed : 17914355). Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis (PubMed : 17914355). In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination (PubMed : 17914355). Prefers nucleosomal substrates (PubMed : 17914355). Does not deubiquitinate histone H2B (PubMed : 17914355). Also deubiquitinates non-histone proteins, such as ribosomal protein RPS27A : deubiquitination of monoubiquitinated RPS27A promotes maturation of the 40S ribosomal subunit (PubMed : 32129764). Also mediates deubiquitination of tektin proteins (TEKT1, TEKT2, TEK3, TEKT4 and TEKT5), promoting their stability.
See full target information USP16
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