Recombinant human USP20 protein (Active) is a Human Fragment protein, in the 120 to 914 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
K A V P I A V A D E G E S E S E D D D L K P R G L T G M K N L G N S C Y M N A A L Q A L S N C P P L T Q F F L E C G G L V R T D K K P A L C K S Y Q K L V S E V W H K K R P S Y V V P T S L S H G I K L V N P M F R G Y A Q Q D T Q E F L R C L M D Q L H E E L K E P V V A T V A L T E A R D S D S S D T D E K R E G D R S P S E D E F L S C D S S S D R G E G D G Q G R G G G S S Q A E T E L L I P D E A G R A I S E K E R M K D R K F S W G Q Q R T N S E Q V D E D A D V D T A M A A L D D Q P A E A Q P P S P R S S S P C R T P E P D N D A H L R S S S R P C S P V H H H E G H A K L S S S P P R A S P V R M A P S Y V L K K A Q V L S A G S R R R K E Q R Y R S V I S D I F D G S I L S L V Q C L T C D R V S T T V E T F Q D L S L P I P G K E D L A K L H S A I Y Q N V P A K P G A C G D S Y A A Q G W L A F I V E Y I R R F V V S C T P S W F W G P V V T L E D C L A A F F A A D E L K G D N M Y S C E R C K K L R N G V K Y C K V L R L P E I L C I H L K R F R H E V M Y S F K I N S H V S F P L E G L D L R P F L A K E C T S Q I T T Y D L L S V I C H H G T A G S G H Y I A Y C Q N V I N G Q W Y E F D D Q Y V T E V H E T V V Q N A E G Y V L F Y R K S S E E A M R E R Q Q V V S L A A M R E P S L L R F Y V S R E W L N K F N T F A E P G P I T N Q T F L C S H G G I P P H K Y H Y I D D L V V I L P Q N V W E H L Y N R F G G G P A V N H L Y V C S I C Q V E I E A L A K R R R I E I D T F I K L N K A F Q A E E S P G V I Y C I S M Q W F R E W E A F V K G K D N E P P G P I D N S R I A Q V K G S G H V Q L K Q G A D Y G Q I S E E T W T Y L N S L Y G G G P E I A I R Q S V A Q P L G P E N L H G E Q K I E A E T R A V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Deubiquitinating enzyme that plays a role in many cellular processes including autophagy, cellular antiviral response or membrane protein biogenesis (PubMed:27801882, PubMed:29487085). Attenuates TLR4-mediated NF-kappa-B signaling by cooperating with beta-arrestin-2/ARRB2 and inhibiting TRAF6 autoubiquitination (PubMed:26839314). Promotes cellular antiviral responses by deconjugating 'Lys-33' and 'Lys-48'-linked ubiquitination of STING1 leading to its stabilization (PubMed:27801882). Plays an essential role in autophagy induction by regulating the ULK1 stability through deubiquitination of ULK1 (PubMed:29487085). Acts as a positive regulator for NF-kappa-B activation by TNF-alpha through deubiquitinating 'Lys-48'-linked polyubiquitination of SQSTM1, leading to its increased stability (PubMed:32354117). Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2) (PubMed:19424180). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2 (PubMed:19424180). This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity (PubMed:15776016). Deubiquitinates MCL1, a pivotal member of the anti-apoptotic Bcl-2 protein family to regulate its stability (PubMed:35063767). Within the endoplasmic reticulum, participates with USP33 in the rescue of post-translationally targeted membrane proteins that are inappropriately ubiquitinated by the cytosolic protein quality control in the cytosol (PubMed:33792613).
Alternative names
KIAA1003, LSFR3A, VDU2, USP20, Ubiquitin carboxyl-terminal hydrolase 20, Deubiquitinating enzyme 20, Ubiquitin thioesterase 20, Ubiquitin-specific-processing protease 20, VHL-interacting deubiquitinating enzyme 2, hVDU2
Recommended products
Recombinant human USP20 protein (Active) is a Human Fragment protein, in the 120 to 914 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab269119 was 55 nmol/min/mg in a DUB assay using ubiquitin-based proluciferin substrate.
- Accession
- Q9Y2K6-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- K A V P I A V A D E G E S E S E D D D L K P R G L T G M K N L G N S C Y M N A A L Q A L S N C P P L T Q F F L E C G G L V R T D K K P A L C K S Y Q K L V S E V W H K K R P S Y V V P T S L S H G I K L V N P M F R G Y A Q Q D T Q E F L R C L M D Q L H E E L K E P V V A T V A L T E A R D S D S S D T D E K R E G D R S P S E D E F L S C D S S S D R G E G D G Q G R G G G S S Q A E T E L L I P D E A G R A I S E K E R M K D R K F S W G Q Q R T N S E Q V D E D A D V D T A M A A L D D Q P A E A Q P P S P R S S S P C R T P E P D N D A H L R S S S R P C S P V H H H E G H A K L S S S P P R A S P V R M A P S Y V L K K A Q V L S A G S R R R K E Q R Y R S V I S D I F D G S I L S L V Q C L T C D R V S T T V E T F Q D L S L P I P G K E D L A K L H S A I Y Q N V P A K P G A C G D S Y A A Q G W L A F I V E Y I R R F V V S C T P S W F W G P V V T L E D C L A A F F A A D E L K G D N M Y S C E R C K K L R N G V K Y C K V L R L P E I L C I H L K R F R H E V M Y S F K I N S H V S F P L E G L D L R P F L A K E C T S Q I T T Y D L L S V I C H H G T A G S G H Y I A Y C Q N V I N G Q W Y E F D D Q Y V T E V H E T V V Q N A E G Y V L F Y R K S S E E A M R E R Q Q V V S L A A M R E P S L L R F Y V S R E W L N K F N T F A E P G P I T N Q T F L C S H G G I P P H K Y H Y I D D L V V I L P Q N V W E H L Y N R F G G G P A V N H L Y V C S I C Q V E I E A L A K R R R I E I D T F I K L N K A F Q A E E S P G V I Y C I S M Q W F R E W E A F V K G K D N E P P G P I D N S R I A Q V K G S G H V Q L K Q G A D Y G Q I S E E T W T Y L N S L Y G G G P E I A I R Q S V A Q P L G P E N L H G E Q K I E A E T R A V
- Accession
- Q9Y2K6
- Protein length
- Fragment
- Amino acids
- 120 to 914
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Deubiquitinating enzyme that plays a role in many cellular processes including autophagy, cellular antiviral response or membrane protein biogenesis (PubMed:27801882, PubMed:29487085). Attenuates TLR4-mediated NF-kappa-B signaling by cooperating with beta-arrestin-2/ARRB2 and inhibiting TRAF6 autoubiquitination (PubMed:26839314). Promotes cellular antiviral responses by deconjugating 'Lys-33' and 'Lys-48'-linked ubiquitination of STING1 leading to its stabilization (PubMed:27801882). Plays an essential role in autophagy induction by regulating the ULK1 stability through deubiquitination of ULK1 (PubMed:29487085). Acts as a positive regulator for NF-kappa-B activation by TNF-alpha through deubiquitinating 'Lys-48'-linked polyubiquitination of SQSTM1, leading to its increased stability (PubMed:32354117). Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2) (PubMed:19424180). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2 (PubMed:19424180). This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity (PubMed:15776016). Deubiquitinates MCL1, a pivotal member of the anti-apoptotic Bcl-2 protein family to regulate its stability (PubMed:35063767). Within the endoplasmic reticulum, participates with USP33 in the rescue of post-translationally targeted membrane proteins that are inappropriately ubiquitinated by the cytosolic protein quality control in the cytosol (PubMed:33792613).
- Sequence similarities
Belongs to the peptidase C19 family. USP20/USP33 subfamily.
- Post-translational modifications
Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.
- Subcellular localisation
- Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
USP20 also known as ubiquitin-specific peptidase 20 is an enzyme involved in removing ubiquitin molecules from proteins a process that regulates protein degradation and stabilization. The molecular mass of USP20 is approximately 97 kDa. USP20 is widely expressed in various tissues including brain heart and skeletal muscle indicating its versatile role in different cellular contexts. It deubiquitinates substrates impacting their protein stability and controlling their signal pathways.
- Biological function summary
Removal of ubiquitin from protein substrates is an important function of USP20 which interacts with diverse protein complexes. USP20 modulates stability of multiple proteins by preventing their proteasomal degradation. It is involved in maintaining balance in cellular protein levels which affects cellular activities like cell cycle progression and response to stress. This activity can significantly influence the timing of protein turnover impacting how cells respond to changes.
- Pathways
Deubiquitinating activities of USP20 significantly influence stress response and cellular signaling. USP20 functions within the NF-κB signaling pathway a critical mediator of immune response where it interacts with proteins such as IκBα stabilizing them by removing ubiquitin moieties. This regulatory mechanism is essential for proper NF-κB activation and immune response. Additionally USP20 plays a role in endoplasmic reticulum stress pathways by modulating GRP78 levels highlighting its involvement in protein folding and stress handling.
- Associated diseases and disorders
Dysregulation of USP20 is linked to several health conditions like cancer and neurodegenerative diseases. In cancer USP20 influences tumor progression by interacting with the protein MCL-1 an important factor in cell survival therefore affecting apoptosis. In neurodegenerative diseases disruptions in USP20 function or expression can lead to imbalance of protein homeostasis contributing to protein aggregation a common hallmark in diseases like Alzheimer's. Studying USP20 and its interconnected pathways and proteins offers insights into potential therapeutic strategies for these disorders.
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2 product images
Functional Studies - Recombinant human USP20 protein (Active) (ab269119)
The specific activity of ab269119 was 55 nmol/min/mg in a DUB assay using ubiquitin-based proluciferin substrate.
SDS-PAGE - Recombinant human USP20 protein (Active) (ab269119)
SDS-PAGE analysis of ab269119.
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