Recombinant human USP28 protein (Active) is a Human Fragment protein, in the 155 to 675 aa range, expressed in Escherichia coli, with >70% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
R R V D G W P V G L K N V G N T C W F S A V I Q S L F Q L P E F R R L V L S Y S L P Q N V L E N C R S H T E K R N I M F M Q E L Q Y L F A L M M G S N R K F V D P S A A L D L L K G A F R S S E E Q Q Q D V S E F T H K L L D W L E D A F Q L A V N V N S P R N K S E N P M V Q L F Y G T F L T E G V R E G K P F C N N E T F G Q Y P L Q V N G Y R N L D E C L E G A M V E G D V E L L P S D H S V K Y G Q E R W F T K L P P V L T F E L S R F E F N Q S L G Q P E K I H N K L E F P Q I I Y M D R Y M Y R S K E L I R N K R E C I R K L K E E I K I L Q Q K L E R Y V K Y G S G P A R F P L P D M L K Y V I E F A S T K P A S E S C P P E S D T H M T L P L S S V H C S V S D Q T S K E S T S T E S S S Q D V E S T F S S P E D S L P K S K P L T S S R S S M E M P S Q P A P R T V T D E E I N F V K T C L Q R W R S E I E Q D I Q D L K T C I A S T T Q T I E Q M Y C D P L L R Q V P Y R L H A V L V H E G Q A N A G H Y W A Y I Y N Q P R Q S W L K Y N D I S V T E S S W E E V E R D S Y G G L R N V S A Y C L M Y I N D K L P Y F N A E A A P T E S D Q M S E V E A L S V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells (PubMed:24623306).
Alternative names
KIAA1515, USP28, Ubiquitin carboxyl-terminal hydrolase 28, Deubiquitinating enzyme 28, Ubiquitin thioesterase 28, Ubiquitin-specific-processing protease 28
Recommended products
Recombinant human USP28 protein (Active) is a Human Fragment protein, in the 155 to 675 aa range, expressed in Escherichia coli, with >70% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab269121 was 499 nmol/min/mg in a DUB assay using ubiquitin-based proluciferin substrate.
- Accession
- Q96RU2-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- R R V D G W P V G L K N V G N T C W F S A V I Q S L F Q L P E F R R L V L S Y S L P Q N V L E N C R S H T E K R N I M F M Q E L Q Y L F A L M M G S N R K F V D P S A A L D L L K G A F R S S E E Q Q Q D V S E F T H K L L D W L E D A F Q L A V N V N S P R N K S E N P M V Q L F Y G T F L T E G V R E G K P F C N N E T F G Q Y P L Q V N G Y R N L D E C L E G A M V E G D V E L L P S D H S V K Y G Q E R W F T K L P P V L T F E L S R F E F N Q S L G Q P E K I H N K L E F P Q I I Y M D R Y M Y R S K E L I R N K R E C I R K L K E E I K I L Q Q K L E R Y V K Y G S G P A R F P L P D M L K Y V I E F A S T K P A S E S C P P E S D T H M T L P L S S V H C S V S D Q T S K E S T S T E S S S Q D V E S T F S S P E D S L P K S K P L T S S R S S M E M P S Q P A P R T V T D E E I N F V K T C L Q R W R S E I E Q D I Q D L K T C I A S T T Q T I E Q M Y C D P L L R Q V P Y R L H A V L V H E G Q A N A G H Y W A Y I Y N Q P R Q S W L K Y N D I S V T E S S W E E V E R D S Y G G L R N V S A Y C L M Y I N D K L P Y F N A E A A P T E S D Q M S E V E A L S V
- Accession
- Q96RU2
- Protein length
- Fragment
- Amino acids
- 155 to 675
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells (PubMed:24623306).
- Sequence similarities
Belongs to the peptidase C19 family. USP28 subfamily.
- Post-translational modifications
Degraded upon nickel ion level or hypoxia exposure.
- Subcellular localisation
- Nucleus, Nucleoplasm
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
USP28 also known as Ubiquitin Specific Peptidase 28 is a deubiquitinating enzyme responsible for removing ubiquitin from protein substrates. It belongs to the cysteine protease family and has a molecular mass of approximately 120 kDa. USP28 is mainly expressed in the nucleus and cytoplasm of cells and appears in various tissues including the lung liver and gastrointestinal tract. It plays a role in regulating protein stability by counteracting ubiquitination an important process for protein degradation.
- Biological function summary
This deubiquitinase regulates several cellular processes such as DNA damage response cell cycle progression and apoptosis. USP28 influences protein stability by directly associating with components of the c-MYC signaling pathway. It is known to interact with and stabilize Fbw7 which is part of the ubiquitin ligase complex thereby affecting the turnover of proteins like c-MYC and cyclin E. Through these interactions USP28 helps maintain normal levels of these important regulators for healthy cellular functions.
- Pathways
The enzyme plays an important part in the DNA damage response and MAPK signaling pathways. These pathways ensure proper genomic integrity and cell proliferation. USP28 enhances the stability of the key regulatory protein c-MYC in the p53 pathway emphasizing its role in cell cycle regulation. Its interaction with other proteins like Fbw7 and c-MYC allows it to modulate the rate of cell growth and division indicating its importance in controlled cellular pathways.
- Associated diseases and disorders
USP28 has connections to cancer and neurodegenerative diseases. Its interactions with c-MYC are implicated in tumorigenesis particularly in colorectal and breast cancers due to the stabilization of oncogenic proteins that promote uncontrollable cell proliferation. Additionally USP28 might contribute to neurodegenerative disorders by affecting protein aggregates commonly seen in such diseases although studies are less established in this area. The enzyme's strong connection with proteins like Fbw7 and c-MYC highlights its relevance in these disease situations making it a potential target for therapeutic intervention.
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2 product images
Functional Studies - Recombinant human USP28 protein (Active) (ab269121)
The specific activity of ab269121 was 499 nmol/min/mg in a DUB assay using ubiquitin-based proluciferin substrate.
SDS-PAGE - Recombinant human USP28 protein (Active) (ab269121)
SDS-PAGE analysis of ab269121.
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