Recombinant human USP28 protein (Active)
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Recombinant human USP28 protein (Active) is a Human Fragment protein, in the 155 to 675 aa range, expressed in Escherichia coli, with >70%, suitable for SDS-PAGE, FuncS.
View Alternative Names
KIAA1515, USP28, Ubiquitin carboxyl-terminal hydrolase 28, Deubiquitinating enzyme 28, Ubiquitin thioesterase 28, Ubiquitin-specific-processing protease 28
- FuncS
Supplier Data
Functional Studies - Recombinant human USP28 protein (Active) (AB269121)
The specific activity of ab269121 was 499 nmol/min/mg in a DUB assay using ubiquitin-based proluciferin substrate.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human USP28 protein (Active) (AB269121)
SDS-PAGE analysis of ab269121.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This deubiquitinase regulates several cellular processes such as DNA damage response cell cycle progression and apoptosis. USP28 influences protein stability by directly associating with components of the c-MYC signaling pathway. It is known to interact with and stabilize Fbw7 which is part of the ubiquitin ligase complex thereby affecting the turnover of proteins like c-MYC and cyclin E. Through these interactions USP28 helps maintain normal levels of these important regulators for healthy cellular functions.
Pathways
The enzyme plays an important part in the DNA damage response and MAPK signaling pathways. These pathways ensure proper genomic integrity and cell proliferation. USP28 enhances the stability of the key regulatory protein c-MYC in the p53 pathway emphasizing its role in cell cycle regulation. Its interaction with other proteins like Fbw7 and c-MYC allows it to modulate the rate of cell growth and division indicating its importance in controlled cellular pathways.
Specifications
Form
Liquid
General info
Function
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome : acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells (PubMed : 24623306).
Sequence similarities
Belongs to the peptidase C19 family. USP28 subfamily.
Post-translational modifications
Degraded upon nickel ion level or hypoxia exposure.. Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR (PubMed:16901786). Phosphorylated by PRKD1 (PubMed:24623306).
Subcellular localisation
Nucleus
Target data
Complementary Products
Product promise
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