Recombinant Human USP6 protein is a Human Fragment protein, in the 2 to 89 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
D M V E N A D S L Q A Q E R K D I L M K Y D K G H R A G L P E D K G P E P V G I N S S I D R F G I L H E T E L P P V T A R E A K K I R R E M T R T S K W M E M L G E W E T Y K H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation. May act as a GTPase-activating protein for RAB3A (PubMed:19077034).
Alternative names
HRP1, TRE2, USP6, Ubiquitin carboxyl-terminal hydrolase 6, Deubiquitinating enzyme 6, Proto-oncogene TRE-2, RN-tre, Ubiquitin thioesterase 6, Ubiquitin-specific-processing protease 6
Recommended products
Recombinant Human USP6 protein is a Human Fragment protein, in the 2 to 89 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P35125-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- D M V E N A D S L Q A Q E R K D I L M K Y D K G H R A G L P E D K G P E P V G I N S S I D R F G I L H E T E L P P V T A R E A K K I R R E M T R T S K W M E M L G E W E T Y K H
- Accession
- P35125
- Protein length
- Fragment
- Amino acids
- 2 to 89
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation. May act as a GTPase-activating protein for RAB3A (PubMed:19077034).
- Sequence similarities
Belongs to the peptidase C19 family.
- Post-translational modifications
Monubiquitinated; ubiquitination is calmodulin and calcium dependent.
- Subcellular localisation
- Endosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The USP6 protein also known as Tre-2 or Ubiquitin-Specific Peptidase 6 serves as a deubiquitinating enzyme. It has a molecular mass of approximately 140 kDa. USP6 catalyzes the removal of ubiquitin from specific substrates impacting their stability and degradation. You can find USP6 expression in a variety of tissues but it primarily shows in skeletal muscle and brain tissues.
- Biological function summary
USP6 regulates numerous cellular processes by modifying protein ubiquitination levels. This protein often interacts with components of the endosomal sorting complex required for transport (ESCRT) playing a part in the regulation of membrane trafficking. Additionally USP6 influences cell signaling pathways by modulating the turnover of receptor proteins important for cell communication.
- Pathways
Several cellular networks involve USP6 that control protein degradation and signal transduction. In particular it plays a role in the Wnt signaling pathway impacting cell proliferation and differentiation. The protein also associates with β-catenin through this pathway affecting cellular processes such as transcriptional activation and cell cycle regulation.
- Associated diseases and disorders
Aberrant USP6 expression links with various conditions. It has a connection to aneurysmal bone cysts where its upregulation results in tumor formation due to enhanced osteoclast activity. The USP6 gene fusion with another protein CDH11 is implicated in this disorder altering normal cellular signaling. Furthermore USP6's dysregulation can also relate to certain soft tissue tumors indicating its role in tumorigenesis.
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1 product image
SDS-PAGE - Recombinant Human USP6 protein (ab160339)
ab160339 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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