Recombinant Human VAT1 protein is a Human Full Length protein, in the 1 to 393 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M S D E R E V A E A A T G E D A S S P P P K T E A A S D P Q H P A A S E G A A A A A A S P P L L R C L V L T G F G G Y D K V K L Q S R P A A P P A P G P G Q L T L R L R A C G L N F A D L M A R Q G L Y D R L P P L P V T P G M E G A G V V I A V G E G V S D R K A G D R V M V L N R S G M W Q E E V T V P S V Q T F L I P E A M T F E E A A A L L V N Y I T A Y M V L F D F G N L Q P G H S V L V H M A A G G V G M A A V Q L C R T V E N V T V F G T A S A S K H E A L K E N G V T H P I D Y H T T D Y V D E I K K I S P K G V D I V M D P L G G S D T A K G Y N L L K P M G K V V T Y G M A N L L T G P K R N L M A L A R T W W N Q F S V T A L Q L L Q A N R A V C G F H L G Y L D G E V E L V S G V V A R L L A L Y N Q G H I K P H I D S V W P F E K V A D A M K Q M Q E K K N V G K V L L V P G P E K E N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Possesses ATPase activity (By similarity). Plays a part in calcium-regulated keratinocyte activation in epidermal repair mechanisms. Has no effect on cell proliferation. Negatively regulates mitochondrial fusion in cooperation with mitofusin proteins (MFN1-2).
Alternative names
Synaptic vesicle membrane protein VAT-1 homolog, VAT1
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Recombinant Human VAT1 protein is a Human Full Length protein, in the 1 to 393 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q99536-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M S D E R E V A E A A T G E D A S S P P P K T E A A S D P Q H P A A S E G A A A A A A S P P L L R C L V L T G F G G Y D K V K L Q S R P A A P P A P G P G Q L T L R L R A C G L N F A D L M A R Q G L Y D R L P P L P V T P G M E G A G V V I A V G E G V S D R K A G D R V M V L N R S G M W Q E E V T V P S V Q T F L I P E A M T F E E A A A L L V N Y I T A Y M V L F D F G N L Q P G H S V L V H M A A G G V G M A A V Q L C R T V E N V T V F G T A S A S K H E A L K E N G V T H P I D Y H T T D Y V D E I K K I S P K G V D I V M D P L G G S D T A K G Y N L L K P M G K V V T Y G M A N L L T G P K R N L M A L A R T W W N Q F S V T A L Q L L Q A N R A V C G F H L G Y L D G E V E L V S G V V A R L L A L Y N Q G H I K P H I D S V W P F E K V A D A M K Q M Q E K K N V G K V L L V P G P E K E N
- Accession
- Q99536
- Protein length
- Full Length
- Predicted molecular weight
- 44.1 kDa
- Amino acids
- 1 to 393
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab123198 is purified using conventional chromatography techniques.
General info
- Function
Possesses ATPase activity (By similarity). Plays a part in calcium-regulated keratinocyte activation in epidermal repair mechanisms. Has no effect on cell proliferation. Negatively regulates mitochondrial fusion in cooperation with mitofusin proteins (MFN1-2).
- Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
- Subcellular localisation
- Mitochondrion outer membrane
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VAT1 also known as Vesicle Amine Transport Protein 1 homolog is a protein involved mechanically in the transport and storage of amines in synaptic vesicles. It has an approximate mass of 45 kDa. VAT1 is highly expressed in the nervous system particularly in regions with a high density of synaptic vesicles. It localizes to the membrane of synaptic vesicles suggesting its role in vesicular trafficking and neurotransmitter transport processes.
- Biological function summary
VAT1 functions in vesicular transport and neurotransmitter storage playing a role in synaptic transmission. This protein does not work alone; it contributes to a larger complex involved in establishing proper neurotransmitter concentrations within synaptic vesicles. VAT1 may also participate in modulating synaptic plasticity which influences learning and memory processes. Its activity ensures proper nerve signal transmission across synapses by maintaining neurotransmitter levels.
- Pathways
The VAT1 protein is important in neurotransmitter release and recycling pathways in the nervous system. It associates with proteins like synaptophysin and synapsin I to facilitate vesicle docking and release. VAT1 plays a role in the synaptic vesicle cycle which includes vesicle loading exocytosis and recycling. Its function guarantees that neurotransmitters are quickly replenished promoting efficient synaptic communication.
- Associated diseases and disorders
VAT1 associates with neurological disorders such as Parkinson’s disease and epilepsy where neurotransmitter imbalances are evident. Connections between VAT1 and the protein alpha-synuclein are apparent in the context of Parkinson’s disease as disruptions in vesicular transport can lead to toxic protein aggregation. In epilepsy disturbances in VAT1 function can impact synaptic efficacy and neurotransmitter availability contributing to seizure activity. Understanding VAT1 function can provide insights into these disorders and offer targets for therapeutic intervention.
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1 product image
SDS-PAGE - Recombinant Human VAT1 protein (ab123198)
15% SDS-PAGE showing ab123198 (3μg).
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