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AB176044

Recombinant Human VAV1 protein

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Recombinant Human VAV1 protein is a Human Fragment protein, in the 189 to 565 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

VAV, VAV1, Proto-oncogene vav

1 Images
SDS-PAGE - Recombinant Human VAV1 protein (AB176044)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human VAV1 protein (AB176044)

3µg of ab176044 by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P15498

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris-HCl buffer, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMTEYDKRCCCLREIQQTEEKYTDTLGSIQQHFLKPLQRFLKPQDIEIIFINIEDLLRVHTHFLKEMKEALGTPGAANLYQVFIKYKERFLVYGRYCSQVESASKHLDRVAAAREDVQMKLEECSQRANNGRFTLRDLLMVPMQRVLKYHLLLQELVKHTQEAMEKENLRLALDAMRDLAQCVNEVKRDNETLRQITNFQLSIENLDQSLAHYGRPKIDGELKITSVERRSKMDRYAFLLDKALLICKRRGDSYDLKDFVNLHSFQVRDDSSGDRDNKKWSHMFLLIEDQGAQGYELFFKTRELKKKWMEQFEMAISNIYPENATANGHDFQMFSFEETTSCKACQMLLRGTFYQGYRCHRCRASAHKECLGRVPPCG","proteinLength":"Fragment","predictedMolecularWeight":"46.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":565,"aminoAcidStart":189,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P15498","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VAV1 also known as VAV-1 is a guanine nucleotide exchange factor with a mass of approximately 95 kDa. It activates Rho family GTPases by promoting the exchange of GDP for GTP. VAV1 is mainly expressed in hematopoietic cells playing an important role in immune responses. Its structure includes several domains – Dbl homology pleckstrin homology and an SH3 domain – that contribute to its function by facilitating interactions with other proteins.
Biological function summary

VAV1 regulates the signaling processes that lead to actin cytoskeletal rearrangements cell proliferation and differentiation. It forms part of larger multiprotein complexes that transmit signals from the cell surface to the nucleus. The activity of VAV1 is important for normal lymphocyte development and activation. By interacting with surface receptors it influences downstream effects altering cellular behavior and promoting adaptive immune responses.

Pathways

VAV1 is integral to the T cell receptor (TCR) signaling pathway and the B cell receptor (BCR) pathway. It acts as a link between the receptors and the Rho family of GTPases such as Rac1 and Cdc42 mediating the transmission of signals that result in cellular responses. The coupling of VAV1 with proteins like LAT and SLP-76 strengthens the signaling cascade necessary for lymphocyte activation and proliferation.

Deregulation of VAV1 is associated with certain cancers such as leukemia and lymphoma. Changes in VAV1 expression or activity can disrupt normal immune cell signaling promoting unchecked growth and survival of malignant cells. Additionally VAV1 is linked to autoimmune diseases where its altered function can lead to inappropriate immune responses. VAV1 interactions with proteins like ZAP-70 highlight its role in these pathologies highlighting its significance as a potential therapeutic target.
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Specifications

Form

Liquid

Additional notes

Purified using conventional chromatography techniques.

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General info

Function

Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.

Post-translational modifications

Phosphorylated on tyrosine residues by HCK in response to IFNG and bacterial lipopolysaccharide (LPS) (By similarity). Phosphorylated by FYN.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
See full target information VAV1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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