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AB139201

Recombinant Human VBP-1 protein

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Recombinant Human VBP-1 protein is a Human Full Length protein, in the 1 to 197 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

PFDN3, VBP1, Prefoldin subunit 3, HIBBJ46, von Hippel-Lindau-binding protein 1, VBP-1, VHL-binding protein 1

1 Images
SDS-PAGE - Recombinant Human VBP-1 protein (AB139201)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human VBP-1 protein (AB139201)

SDS-PAGE of ab139201 (3µg) under reducing condition and visualized by coomassie blue stain.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P61758

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as PFDN3.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAAVKDSCGKGEMATGNGRRLHLGIPEAVFVEDVDSFMKQPGNETADTVLKKLDEQYQKYKFMELNLAQKKRRLKGQIPEIKQTLEILKYMQKKKESTNSMETRFLLADNLYCKASVPPTDKVCLWLGANVMLEYDIDEAQALLEKNLSTATKNLDSLEEDLDFLRDQFTTTEVNMARVYNWDVKRRNKDDSTKNKA","proteinLength":"Full Length","predictedMolecularWeight":"25 kDa","actualMolecularWeight":null,"aminoAcidEnd":197,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P61758","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VBP-1 also known as Von Hippel-Lindau binding protein 1 or prefoldin subunit 3 is a small protein with a molecular mass of approximately 23 kDa. It is expressed in various tissues including the brain heart and skeletal muscle. Mechanically VBP-1 functions as a molecular chaperone facilitating the proper folding of newly synthesized proteins and preventing the aggregation of misfolded proteins. It interacts with different molecular partners to exert its chaperoning activity.
Biological function summary

VBP-1 plays an important role in protein homeostasis particularly by forming part of the prefoldin complex. This complex works alongside the cytosolic chaperonin complex helping with the proper folding of cytoskeletal proteins such as actin and tubulin. By stabilizing these newly synthesized proteins VBP-1 supports cellular structures and intracellular transport systems reflecting its involvement in maintaining cellular integrity.

Pathways

VBP-1 plays a role in the ubiquitin-proteasome pathway and the cellular stress response pathway. In the ubiquitin-proteasome pathway it partners with proteins like HSP70 to target damaged proteins for degradation. In response to cellular stress VBP-1 helps in protein stabilization to prevent harmful aggregation correlating with HSP90 in maintaining cellular homeostasis during stress conditions.

VBP-1 has connections with neurodegenerative diseases such as Alzheimer's disease due to its role in protein folding and aggregation. Misregulation of VBP-1 can lead to the accumulation of neurotoxic protein aggregates. Additionally VBP-1 has been associated with cancer particularly through its interaction with pVHL (von Hippel-Lindau protein). Dysregulation in VBP-1 activity can result in the disruption of the ubiquitin-proteasome system leading to uncontrolled cell growth and cancer development.
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Specifications

Form

Liquid

Additional notes

ab139201 was purified by using conventional chromatography techniques.

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General info

Function

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

Sequence similarities

Belongs to the prefoldin subunit alpha family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
See full target information VBP1
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