Skip to main content

Recombinant Human VCAM1 protein (Active) is a Human Fragment protein, in the 25 to 698 aa range, expressed in HEK 293, with >=95% purity and <= 0.005 EU/µg endotoxin level.

Be the first to review this product! Submit a review

Images

Mass Spectrometry - Recombinant Human VCAM1 protein (Active) (AB288799), expandable thumbnail
  • Biological Activity - Recombinant Human VCAM1 protein (Active) (AB288799), expandable thumbnail
  • SDS-PAGE - Recombinant Human VCAM1 protein (Active) (AB288799), expandable thumbnail
  • HPLC - Recombinant Human VCAM1 protein (Active) (AB288799), expandable thumbnail

Key facts

Purity

>=95% HPLC

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Biologically active

Yes

Amino acid sequence

F K I E T T P E S R Y L A Q I G D S V S L T C S T T G C E S P F F S W R T Q I D S P L N G K V T N E G T T S T L T M N P V S F G N E H S Y L C T A T C E S R K L E K G I Q V E I Y S F P K D P E I H L S G P L E A G K P I T V K C S V A D V Y P F D R L E I D L L K G D H L M K S Q E F L E D A D R K S L E T K S L E V T F T P V I E D I G K V L V C R A K L H I D E M D S V P T V R Q A V K E L Q V Y I S P K N T V I S V N P S T K L Q E G G S V T M T C S S E G L P A P E I F W S K K L D N G N L Q H L S G N A T L T L I A M R M E D S G I Y V C E G V N L I G K N R K E V E L I V Q E K P F T V E I S P G P R I A A Q I G D S V M L T C S V M G C E S P S F S W R T Q I D S P L S G K V R S E G T N S T L T L S P V S F E N E H S Y L C T V T C G H K K L E K G I Q V E L Y S F P R D P E I E M S G G L V N G S S V T V S C K V P S V Y P L D R L E I E L L K G E T I L E N I E F L E D T D M K S L E N K S L E M T F I P T I E D T G K A L V C Q A K L H I D D M E F E P K Q R Q S T Q T L Y V N V A P R D T T V L V S P S S I L E E G S S V N M T C L S Q G F P A P K I L W S R Q L P N G E L Q P L S E N A T L T L I S T K M E D S G V Y L C E G I N Q A G R S R K E V E L I I Q V T P K D I K L T A F P S E S V K E G D T V I I S C T C G N V P E T W I I L K K K A E T G D T V L K S I D G A Y T I R K A Q L K D A G V Y E C E S K N K V G S Q L R S L T L D V Q G R E N N K D Y F S P E

Target data

Function

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed:31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed:10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed:22970700). Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed:35210567).

Alternative names

Recommended products

Recombinant Human VCAM1 protein (Active) is a Human Fragment protein, in the 25 to 698 aa range, expressed in HEK 293, with >=95% purity and <= 0.005 EU/µg endotoxin level.

Key facts

Purity

>=95% HPLC

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Biological activity

Addition of PMA-induced U937 cells to human VCAM-1 coated plates (1.25 μg/mL, 100 μL/well) led to 74.12% cell adherence specifically. Statistical analysis was performed using two-tailed unpaired t-test, ***=p<0.0002.

Accession
P19320-1
Animal free

No

Species

Human

Reconstitution

Reconstitute in PBS

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence

F K I E T T P E S R Y L A Q I G D S V S L T C S T T G C E S P F F S W R T Q I D S P L N G K V T N E G T T S T L T M N P V S F G N E H S Y L C T A T C E S R K L E K G I Q V E I Y S F P K D P E I H L S G P L E A G K P I T V K C S V A D V Y P F D R L E I D L L K G D H L M K S Q E F L E D A D R K S L E T K S L E V T F T P V I E D I G K V L V C R A K L H I D E M D S V P T V R Q A V K E L Q V Y I S P K N T V I S V N P S T K L Q E G G S V T M T C S S E G L P A P E I F W S K K L D N G N L Q H L S G N A T L T L I A M R M E D S G I Y V C E G V N L I G K N R K E V E L I V Q E K P F T V E I S P G P R I A A Q I G D S V M L T C S V M G C E S P S F S W R T Q I D S P L S G K V R S E G T N S T L T L S P V S F E N E H S Y L C T V T C G H K K L E K G I Q V E L Y S F P R D P E I E M S G G L V N G S S V T V S C K V P S V Y P L D R L E I E L L K G E T I L E N I E F L E D T D M K S L E N K S L E M T F I P T I E D T G K A L V C Q A K L H I D D M E F E P K Q R Q S T Q T L Y V N V A P R D T T V L V S P S S I L E E G S S V N M T C L S Q G F P A P K I L W S R Q L P N G E L Q P L S E N A T L T L I S T K M E D S G V Y L C E G I N Q A G R S R K E V E L I I Q V T P K D I K L T A F P S E S V K E G D T V I I S C T C G N V P E T W I I L K K K A E T G D T V L K S I D G A Y T I R K A Q L K D A G V Y E C E S K N K V G S Q L R S L T L D V Q G R E N N K D Y F S P E

Accession

P19320

Protein length

Fragment

Amino acids

25 to 698

Nature

Recombinant

Specifications

Form

Lyophilized

General info

Function

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed:31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed:10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed:22970700). Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed:35210567).

Post-translational modifications

Cleaved by the metalloproteinase ADAM17 to generate the soluble form.

Storage

Shipped at conditions

Ambient - Cannot Ship with Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.

Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Associated diseases and disorders

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Mass Spectrometry - Recombinant Human VCAM1 protein (Active) (ab288799), expandable thumbnail

    Mass Spectrometry - Recombinant Human VCAM1 protein (Active) (ab288799)

    Mass determination by ESI-TOF.

    Predicted MW is 74284.60 Da. (+/- 10 Da by ESI-TOF). Observed MW is 74291.89 Da.

  • Biological Activity - Recombinant Human VCAM1 protein (Active) (ab288799), expandable thumbnail

    Biological Activity - Recombinant Human VCAM1 protein (Active) (ab288799)

    Addition of PMA-induced U937 cells to human VCAM-1 coated plates (1.25 μg/mL, 100 μL/well) led to 74.12% cell adherence specifically. Statistical analysis was performed using two-tailed unpaired t-test, ***=p<0.0002.

  • SDS-PAGE - Recombinant Human VCAM1 protein (Active) (ab288799), expandable thumbnail

    SDS-PAGE - Recombinant Human VCAM1 protein (Active) (ab288799)

    SDS-PAGE analysis of ab288799

  • HPLC - Recombinant Human VCAM1 protein (Active) (ab288799), expandable thumbnail

    HPLC - Recombinant Human VCAM1 protein (Active) (ab288799)

    HPLC analysis of ab288799

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com