Recombinant Human VCAM1 protein (Active)
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- What is this?
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Recombinant Human VCAM1 protein (Active) is a Human Fragment protein, in the 25 to 698 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for FuncS.
View Alternative Names
CD106, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, VCAM1
- Biological Activity
Lab
Biological Activity - Recombinant Human VCAM1 protein (Active) (AB288799)
Addition of PMA-induced U937 cells to human VCAM-1 coated plates (1.25 μg/mL, 100 μL/well) led to 74.12% cell adherence specifically. Statistical analysis was performed using two-tailed unpaired t-test, ***=p<0.0002.
- Mass Spec
Supplier Data
Mass Spectrometry - Recombinant Human VCAM1 protein (Active) (AB288799)
Mass determination by ESI-TOF.
Predicted MW is 74284.60 Da. (+/- 10 Da by ESI-TOF). Observed MW is 74291.89 Da.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human VCAM1 protein (Active) (AB288799)
SDS-PAGE analysis of ab288799
- HPLC
Supplier Data
HPLC - Recombinant Human VCAM1 protein (Active) (AB288799)
HPLC analysis of ab288799
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.
Pathways
VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.
Specifications
Form
Lyophilized
General info
Function
Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed : 31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed : 10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed : 22970700). Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed : 35210567).
Post-translational modifications
Cleaved by the metalloproteinase ADAM17 to generate the soluble form.. Sialoglycoprotein.. Ubiquitinated by TRIM65 via 'Lys-48'-linked ubiquitination; leading to proteasomal degradation.
Target data
Complementary Products
Primary Antibodies
AB134047
BOND RX™ Validated|RabMAb|Recombinant|KO Validated
![Western blot - Anti-VCAM1 antibody [EPR5047] (AB134047)](https://content.abcam.com/products/images/vcam1-antibody-epr5047-ab134047--western-blot-img56771.jpg)
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Product promise
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