Recombinant Human VCAM1 protein (Active) is a Human Fragment protein, in the 25 to 698 aa range, expressed in HEK 293, with >=95% purity and <= 0.005 EU/µg endotoxin level.
>=95% HPLC
<= 0.005 EU/µg
HEK 293 cells
Tag free
Yes
F K I E T T P E S R Y L A Q I G D S V S L T C S T T G C E S P F F S W R T Q I D S P L N G K V T N E G T T S T L T M N P V S F G N E H S Y L C T A T C E S R K L E K G I Q V E I Y S F P K D P E I H L S G P L E A G K P I T V K C S V A D V Y P F D R L E I D L L K G D H L M K S Q E F L E D A D R K S L E T K S L E V T F T P V I E D I G K V L V C R A K L H I D E M D S V P T V R Q A V K E L Q V Y I S P K N T V I S V N P S T K L Q E G G S V T M T C S S E G L P A P E I F W S K K L D N G N L Q H L S G N A T L T L I A M R M E D S G I Y V C E G V N L I G K N R K E V E L I V Q E K P F T V E I S P G P R I A A Q I G D S V M L T C S V M G C E S P S F S W R T Q I D S P L S G K V R S E G T N S T L T L S P V S F E N E H S Y L C T V T C G H K K L E K G I Q V E L Y S F P R D P E I E M S G G L V N G S S V T V S C K V P S V Y P L D R L E I E L L K G E T I L E N I E F L E D T D M K S L E N K S L E M T F I P T I E D T G K A L V C Q A K L H I D D M E F E P K Q R Q S T Q T L Y V N V A P R D T T V L V S P S S I L E E G S S V N M T C L S Q G F P A P K I L W S R Q L P N G E L Q P L S E N A T L T L I S T K M E D S G V Y L C E G I N Q A G R S R K E V E L I I Q V T P K D I K L T A F P S E S V K E G D T V I I S C T C G N V P E T W I I L K K K A E T G D T V L K S I D G A Y T I R K A Q L K D A G V Y E C E S K N K V G S Q L R S L T L D V Q G R E N N K D Y F S P E
Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed:31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed:10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed:22970700). Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed:35210567).
Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, VCAM1
Recombinant Human VCAM1 protein (Active) is a Human Fragment protein, in the 25 to 698 aa range, expressed in HEK 293, with >=95% purity and <= 0.005 EU/µg endotoxin level.
>=95% HPLC
<= 0.005 EU/µg
HEK 293 cells
Tag free
Yes
Addition of PMA-induced U937 cells to human VCAM-1 coated plates (1.25 μg/mL, 100 μL/well) led to 74.12% cell adherence specifically. Statistical analysis was performed using two-tailed unpaired t-test, ***=p<0.0002.
No
Human
Reconstitute in PBS
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
F K I E T T P E S R Y L A Q I G D S V S L T C S T T G C E S P F F S W R T Q I D S P L N G K V T N E G T T S T L T M N P V S F G N E H S Y L C T A T C E S R K L E K G I Q V E I Y S F P K D P E I H L S G P L E A G K P I T V K C S V A D V Y P F D R L E I D L L K G D H L M K S Q E F L E D A D R K S L E T K S L E V T F T P V I E D I G K V L V C R A K L H I D E M D S V P T V R Q A V K E L Q V Y I S P K N T V I S V N P S T K L Q E G G S V T M T C S S E G L P A P E I F W S K K L D N G N L Q H L S G N A T L T L I A M R M E D S G I Y V C E G V N L I G K N R K E V E L I V Q E K P F T V E I S P G P R I A A Q I G D S V M L T C S V M G C E S P S F S W R T Q I D S P L S G K V R S E G T N S T L T L S P V S F E N E H S Y L C T V T C G H K K L E K G I Q V E L Y S F P R D P E I E M S G G L V N G S S V T V S C K V P S V Y P L D R L E I E L L K G E T I L E N I E F L E D T D M K S L E N K S L E M T F I P T I E D T G K A L V C Q A K L H I D D M E F E P K Q R Q S T Q T L Y V N V A P R D T T V L V S P S S I L E E G S S V N M T C L S Q G F P A P K I L W S R Q L P N G E L Q P L S E N A T L T L I S T K M E D S G V Y L C E G I N Q A G R S R K E V E L I I Q V T P K D I K L T A F P S E S V K E G D T V I I S C T C G N V P E T W I I L K K K A E T G D T V L K S I D G A Y T I R K A Q L K D A G V Y E C E S K N K V G S Q L R S L T L D V Q G R E N N K D Y F S P E
Fragment
25 to 698
Recombinant
Lyophilized
Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed:31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed:10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed:22970700). Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed:35210567).
Cleaved by the metalloproteinase ADAM17 to generate the soluble form.
Ambient - Cannot Ship with Ice
Ambient
Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
This supplementary information is collated from multiple sources and compiled automatically.
VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.
Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.
VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.
Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Mass determination by ESI-TOF.
Predicted MW is 74284.60 Da. (+/- 10 Da by ESI-TOF). Observed MW is 74291.89 Da.
Addition of PMA-induced U937 cells to human VCAM-1 coated plates (1.25 μg/mL, 100 μL/well) led to 74.12% cell adherence specifically. Statistical analysis was performed using two-tailed unpaired t-test, ***=p<0.0002.
SDS-PAGE analysis of ab288799
HPLC analysis of ab288799
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com