Recombinant Human VE Cadherin protein (Fc Chimera His Tag) is a Human Fragment protein, in the 29 to 599 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
N P A Q R D T H S L L P T H R R Q K R D W I W N Q M H I D E E K N T S L P H H V G K I K S S V S R K N A K Y L L K G E Y V G K V F R V D A E T G D V F A I E R L D R E N I S E Y H L T A V I V D K D T G E N L E T P S S F T I K V H D V N D N W P V F T H R L F N A S V P E S S A V G T S V I S V T A V D A D D P T V G D H A S V M Y Q I L K G K E Y F A I D N S G R I I T I T K S L D R E K Q A R Y E I V V E A R D A Q G L R G D S G T A T V L V T L Q D I N D N F P F F T Q T K Y T F V V P E D T R V G T S V G S L F V E D P D E P Q N R M T K Y S I L R G D Y Q D A F T I E T N P A H N E G I I K P M K P L D Y E Y I Q Q Y S F I V E A T D P T I D L R Y M S P P A G N R A Q V I I N I T D V D E P P I F Q Q P F Y H F Q L K E N Q K K P L I G T V L A M D P D A A R H S I G Y S I R R T S D K G Q F F R V T K K G D I Y N E K E L D R E V Y P W Y N L T V E A K E L D S T G T P T G K E S I V Q V H I E V L D E N D N A P E F A K P Y Q P K V C E N A V H G Q L V L Q I S A I D K D I T P R N V K F K F I L N T E N N F T L T D N H D N T A N I T V K Y G Q F D R E H T K V H F L P V V I S D N G M P S R T G T S T L T V A V C K C N E Q G E F T F C E D M A A Q V G V S I Q L E P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (PubMed:21269602). This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (By similarity). It associates with alpha-catenin forming a link to the cytoskeleton (PubMed:10861224). Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1 (By similarity). Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen (By similarity). These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B (PubMed:20332120). Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction (PubMed:20332120).
Alternative names
CD144, Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CDH5
Recommended products
Recombinant Human VE Cadherin protein (Fc Chimera His Tag) is a Human Fragment protein, in the 29 to 599 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P33151-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- N P A Q R D T H S L L P T H R R Q K R D W I W N Q M H I D E E K N T S L P H H V G K I K S S V S R K N A K Y L L K G E Y V G K V F R V D A E T G D V F A I E R L D R E N I S E Y H L T A V I V D K D T G E N L E T P S S F T I K V H D V N D N W P V F T H R L F N A S V P E S S A V G T S V I S V T A V D A D D P T V G D H A S V M Y Q I L K G K E Y F A I D N S G R I I T I T K S L D R E K Q A R Y E I V V E A R D A Q G L R G D S G T A T V L V T L Q D I N D N F P F F T Q T K Y T F V V P E D T R V G T S V G S L F V E D P D E P Q N R M T K Y S I L R G D Y Q D A F T I E T N P A H N E G I I K P M K P L D Y E Y I Q Q Y S F I V E A T D P T I D L R Y M S P P A G N R A Q V I I N I T D V D E P P I F Q Q P F Y H F Q L K E N Q K K P L I G T V L A M D P D A A R H S I G Y S I R R T S D K G Q F F R V T K K G D I Y N E K E L D R E V Y P W Y N L T V E A K E L D S T G T P T G K E S I V Q V H I E V L D E N D N A P E F A K P Y Q P K V C E N A V H G Q L V L Q I S A I D K D I T P R N V K F K F I L N T E N N F T L T D N H D N T A N I T V K Y G Q F D R E H T K V H F L P V V I S D N G M P S R T G T S T L T V A V C K C N E Q G E F T F C E D M A A Q V G V S I Q L E P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K H H H H H H
- Accession
- P33151
- Protein length
- Fragment
- Predicted molecular weight
- 91.5 kDa
- Amino acids
- 29 to 599
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (PubMed:21269602). This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (By similarity). It associates with alpha-catenin forming a link to the cytoskeleton (PubMed:10861224). Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1 (By similarity). Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen (By similarity). These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B (PubMed:20332120). Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction (PubMed:20332120).
- Post-translational modifications
Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB (By similarity).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VE Cadherin also known as CD144 or Cadherin-5 is a protein of about 135 kDa involved in cell adhesion. It is a transmembrane protein characterized by its presence at endothelial cell junctions. VE Cadherin belongs to the cadherin superfamily and its expression predominantly occurs in vascular endothelial cells. This protein facilitates the adhesion between adjacent endothelial cells maintaining the integrity of endothelial barriers.
- Biological function summary
VE Cadherin performs essential roles in vascular development and permeability. It is a component of adherens junctions and connects with catenins to mediate cell-cell adhesion through calcium-dependent binding. This protein's adhesive function is critical for blood vessel maturation and response to inflammatory stimuli. Altering VE Cadherin expression impacts vascular stability and endothelial cell layering.
- Pathways
VE Cadherin interacts with the Wnt and VEGF signaling pathways. It plays a role in the regulation of endothelial cell survival and migration. The interaction with β-catenin is important in the Wnt pathway influencing the transcription of target genes that modulate cell behavior. In the VEGF pathway VE Cadherin's association with protein kinase C highlights its involvement in endothelial permeability and angiogenesis processes.
- Associated diseases and disorders
VE Cadherin dysregulation links to cancer progression and atherosclerosis. Its role in maintaining endothelial barrier integrity implicates it in tumor metastasis where disrupted cell adhesion facilitates cancer cell dissemination. Additionally altered VE Cadherin expression or function associates with atherosclerotic disease where damaged vascular endothelium leads to plaque formation. The connection of VE Cadherin with β-catenin in these conditions highlights its significance in pathological angiogenesis and vascular remodeling.
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SDS-PAGE - Recombinant Human VE Cadherin protein (Fc Chimera His Tag) (ab224783)
15% SDS-PAGE analysis of 3 μg ab224783.
MW: 70-100 kDa (SDS-PAGE under reducing conditions).
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