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AB42583

Recombinant human VE-PTP protein (GST tag N-Terminus)

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Recombinant human VE-PTP protein (GST tag N-Terminus) is a Human Fragment protein, in the 1675 to 1996 aa range, expressed in Escherichia coli, with >95%, suitable for FuncS, Inhib.

View Alternative Names

PTPB, PTPRB, Receptor-type tyrosine-protein phosphatase beta, Protein-tyrosine phosphatase beta, R-PTP-beta, Vascular endothelial protein tyrosine phosphatase, VE-PTP

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

GST tag N-Terminus

Applications

FuncS, Inhib

applications

Biologically active

Yes

Biological activity

Specific Activity: 40 U/μg. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3mM DTT, 100 mM NaCl, 50 mM pNPP.

Accession

P23467

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.643% Sodium chloride, 0.6304% Tris HCl, 0.492% Glutathione, 0.0463% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0164% Potassium chloride

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Complementary Products

Primary Antibodies

AB231670

Anti-VE-PTP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE-PTP antibody (AB231670)

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Proteins & Peptides

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Proteins & Peptides

AB140732

Recombinant Human YOD1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human YOD1 protein (His tag N-Terminus) (AB140732)

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Proteins & Peptides

AB259412

Recombinant human VEGF 165A protein (Active)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Inhib": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Protein previously labeled as PTPRB.
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"63.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":1996,"aminoAcidStart":1675,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P23467","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VE-PTP also known as vascular endothelial protein tyrosine phosphatase is an enzyme with a mass of approximately 300 kDa. This protein is part of the receptor-type protein tyrosine phosphatase family and it mainly localizes at endothelial cell junctions. VE-PTP plays an important role in maintaining endothelial cell permeability by dephosphorylating specific tyrosine residues on target proteins such as the VE-cadherin complex. Its expression confines mostly to vascular endothelial cells where it is involved in processes affecting vascular stability and signaling.
Biological function summary

The function of this protein extends to its involvement in the modulation of cell adhesion through its interaction with adherens junctions complexes. VE-PTP associates with complexes like VE-cadherin and plakoglobin to regulate cell–cell junctions influencing the integrity and permeability of the vascular endothelium. This activity is important in processes such as angiogenesis and the maintenance of vascular barrier function making it a significant player in vascular biology.

Pathways

The participation of VE-PTP is notably within the angiogenesis and vascular barrier regulation pathways. The protein governs these pathways by regulating signals through interactions with proteins like VE-cadherin and VEGFR2 which are essential in vascular development and response to growth factors. Its phosphatase activity moderates the phosphorylation state of these key partners thereby influencing the downstream signaling events vital for endothelial function and vascular homeostasis.

The function of VE-PTP associates with pathological conditions such as cancer and cardiovascular diseases. In cancer its role in angiogenesis can contribute to tumor vascularization impacting tumor growth and metastasis. Meanwhile in cardiovascular diseases its influence on endothelial permeability can affect atherosclerotic plaque stability and contribute to vascular complications. Interacting proteins such as VEGFR2 are also implicated in these disorders linking VE-PTP to these disease pathways through their regulatory relationships in endothelial dynamics.
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Specifications

Form

Liquid

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General info

Function

Plays an important role in blood vessel remodeling and angiogenesis. Not necessary for the initial formation of blood vessels, but is essential for their maintenance and remodeling. Can induce dephosphorylation of TEK/TIE2, CDH5/VE-cadherin and KDR/VEGFR-2. Regulates angiopoietin-TIE2 signaling in endothelial cells. Acts as a negative regulator of TIE2, and controls TIE2 driven endothelial cell proliferation, which in turn affects blood vessel remodeling during embryonic development and determines blood vessel size during perinatal growth. Essential for the maintenance of endothelial cell contact integrity and for the adhesive function of VE-cadherin in endothelial cells and this requires the presence of plakoglobin (By similarity).

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.

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Product protocols

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Target data

Plays an important role in blood vessel remodeling and angiogenesis. Not necessary for the initial formation of blood vessels, but is essential for their maintenance and remodeling. Can induce dephosphorylation of TEK/TIE2, CDH5/VE-cadherin and KDR/VEGFR-2. Regulates angiopoietin-TIE2 signaling in endothelial cells. Acts as a negative regulator of TIE2, and controls TIE2 driven endothelial cell proliferation, which in turn affects blood vessel remodeling during embryonic development and determines blood vessel size during perinatal growth. Essential for the maintenance of endothelial cell contact integrity and for the adhesive function of VE-cadherin in endothelial cells and this requires the presence of plakoglobin (By similarity).
See full target information PTPRB
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